Effects of Different Carbon Sources on Mycelium Growth and Extracellular Enzyme Activities of Agaricus bisporus A15 and W192

WANG Qian; XIAO Tingting; ZHANG Yuchen; ZHANG Jinjing; JUAN Jiaxiang; CHEN Hui; CHEN Mingjie; HUANG Jianchun

doi:10.11924/j.issn.1000-6850.casb2024-0338

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Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (9) : 140-148. DOI: 10.11924/j.issn.1000-6850.casb2024-0338

Effects of Different Carbon Sources on Mycelium Growth and Extracellular Enzyme Activities of Agaricus bisporus A15 and W192

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Abstract

The effects of different carbon sources on the mycelium growth and extracellular enzyme activities of A15 and W192 were investigated to understand the physiological requirements of carbohydrates of the two commercial Agaricus bisporus strains. Using different monosaccharides, disaccharides, oligosaccharides and polysaccharides as test materials, the mycelial growth rate, mycelial biomass and extracellular lignocellulase activities were determined. Glucose and fructose increased the mycelial biomass of A15 by 7.7%-30.8% and 23.1%-38.5%, respectively, while that of W192 was increased by 21.1%-36.8% and 26.3%-57.9%, respectively. Trehalose, sucrose, starch and cellulose increased the growth rate of mycelia; trehalose, maltose, sucrose, xylo-oligosaccharide, starch and cellulose increased mycelial biomass, among which 0.5% cellulose increased the mycelial biomass of A15 and W192 by 3.3 and 2.3 times, respectively. 10 carbon sources increased laccase, C1 and Cx activities of A15 and W192, respectively. All the 12 carbon sources inhibited β-GC activity of A15. However, in addition to xylan and lignin, the β-GC activity of W192 was increased. Trehalose, maltose, sucrose, xylo-oligosaccharide, starch and microcrystalline cellulose promote mycelial growth significantly. W192 may have higher carbon source demand or tolerance than A15.

Key words

Agaricus bisporus / commercial stains / A15 / W192 / carbon source / mycelial growth / extracellular enzyme

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WANG Qian , XIAO Tingting , ZHANG Yuchen , et al . Effects of Different Carbon Sources on Mycelium Growth and Extracellular Enzyme Activities of Agaricus bisporus A15 and W192[J]. Chinese Agricultural Science Bulletin. 2025, 41(9): 140-148 https://doi.org/10.11924/j.issn.1000-6850.casb2024-0338

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