人兽共患寄生虫种类多、宿主广泛且危害严重。血吸虫病、棘球蚴病、囊尾蚴病、旋毛虫病、弓形虫病等是常见的重要人兽共患寄生虫病。人类和家畜饱受寄生虫病的危害，这对公共卫生和畜牧业造成了很大的影响。控制传染源、切断传播途径和保护易感群是控制人兽共患寄生虫病流行的综合防控措施。在综合防控策略中，疫苗的使用是切断循环链、控制乃至消灭人兽共患寄生虫病的理想和有效途径之一。选用高效的抗原筛选方法挖掘潜在的疫苗候选分子是开发疫苗的前提和关键。抗原筛选技术的更新换代使得研究者发掘出了更多新抗原和保护性多肽。现有的抗原筛选方法主要包括传统的粗抗原筛选法、cDNA文库筛选法、蛋白质组学筛选法、生物信息学及多组学技术联合筛选法。很多抗原筛选的方法是伴随寄生虫疫苗研究的发展应运而生的，粗抗原筛选法是基于抗原抗体相互反应的免疫学原理而设计的，此方法筛选的天然抗原可引起机体较强的免疫反应；cDNA文库筛选抗原的优势在于筛选更有针对性，所以候选产物的成分更单一、明确；蛋白质组学筛选法是基于质谱而兴起的一种筛选技术，它既可对未知蛋白组分进行鉴定，还可对鉴定结果进行差异比较，在未知分子的发现和功能特殊的靶分子筛选中发挥着重要作用；随着后基因时代的到来，生物信息学及多组学联合筛选技术使得抗原筛选逐步进入了多维、立体的筛选模式，也使得候选抗原及其表位的功能研究更加深入，这为基因工程疫苗和多肽疫苗候选分子的筛选提供了技术手段。

The endemic foci of human trichinellosis are mainly located in southwestern China. Seroepidemiological surveys of Trichinella spiralis infection in humans were carried out in 10 out of 34 Provinces/Autonomous Regions/Municipals (P/A/M) of China during 2004-2009. The overall seroprevalence was 3.19% (3198/100,282). The highest seroprevalences were mainly located in western China: 8.43% in Yunnan, 6.37% in Inner Mongolia and 5.35% in Sichuan. The seroprevalence of Trichinella infection in humans was related to the habit of eating meat and differed among nationalities. From 2004 to 2009, 15 outbreaks of human trichinellosis, consisting of 1387 cases and four deaths, were reported in the three southwestern-most P/A of China (nine outbreaks in Yunnan, two in Sichuan and four in Tibet), where ethnic groups routinely eat raw meat. Pork is the predominant source of outbreaks of human trichinellosis in China. Out of 15 outbreaks, 12 (85.71%) were caused by eating raw or undercooked pork, and 2 (13.33%) resulted from the consumption of raw wild boar, suggesting the significance of game meat as a source of infection for human trichinellosis. An outbreak of imported trichinellosis involving 49 cases in Yunnan during December 2006 from Laos is the first recorded outbreak of imported trichinellosis in China, but the source of that outbreak could not be identified. The mandatory inspection of pork should be further strengthened in southwestern China.Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.

The 14-3-3 family proteins are vital scaffold proteins that ubiquitously expressed in various tissues. They interact with numerous protein targets and mediate many cellular signaling pathways. The 14-3-3 binding motifs are often embedded in intrinsically disordered regions which are closely associated with liquid-liquid phase separation (LLPS). In the past ten years, LLPS has been observed for a variety of proteins and biological processes, indicating that LLPS plays a fundamental role in the formation of membraneless organelles and cellular condensates. While extensive investigations have been performed on 14-3-3 proteins, its involvement in LLPS is overlooked. To date, 14-3-3 proteins have not been reported to undergo LLPS alone or regulate LLPS of their binding partners. To reveal the potential involvement of 14-3-3 proteins in LLPS, in this review, we summarized the LLPS propensity of 14-3-3 binding partners and found that about one half of them may undergo LLPS spontaneously. We further analyzed the phase separation behavior of representative 14-3-3 binders and discussed how 14-3-3 proteins may be involved. By modulating the conformation and valence of interactions and recruiting other molecules, we speculate that 14-3-3 proteins can efficiently regulate the functions of their targets in the context of LLPS. Considering the critical roles of 14-3-3 proteins, there is an urgent need for investigating the involvement of 14-3-3 proteins in the phase separation process of their targets and the underling mechanisms.© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Strongyloides stercoralis is the fourth most important intestinal nematode worldwide. The parasite load and larvae count are often low, thus conventional methods are not sufficiently sensitive to detect the infection. In this study we developed an immunoglobulin G-based enzyme-linked immunosorbent assay (ELISA) method to detect antibodies against S. stercoralis 14-3-3 protein in patients' sera.S. stercoralis RNA was extracted and following complementary DNA synthesis, the 708-bp fragment of 14-3-3 protein was amplified by polymerase chain reaction and cloned into the pET28a+ expression vector. The 30-kDa recombinant 14-3-3 protein was expressed in Escherichia coli BL21 (DE3) cells and purified by affinity chromatography. Finally, its immunoreactivity was assessed by indirect ELISA and western blotting.The S. stercoralis 14-3-3 gene was successfully amplified and cloned into an expression vector. The 30-kDa recombinant protein was purified by affinity chromatography. An ELISA developed in-house detected infected patients' sera with 96% sensitivity.We concluded that the recombinant 14-3-3 protein has enough sensitivity and specificity for detection of strongyloidiasis in human sera and could be applied for serodiagnosis.© The Author(s) 2019. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.