Isolation and Identification of Glarea lozoyensis P1 and Antagonistic and Detoxification Activity Against Fusarium graminearum

GUO Haozhen; ZHOU Huazhong; DENG Chunlin; LI Yan; YANG Lijun; GONG Shuangjun

doi:10.11924/j.issn.1000-6850.casb2024-0431

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Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (12) : 107-112. DOI: 10.11924/j.issn.1000-6850.casb2024-0431

Isolation and Identification of Glarea lozoyensis P1 and Antagonistic and Detoxification Activity Against Fusarium graminearum

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Abstract

This study aims to screen biocontrol strains against Fusarium graminearum species complex in order to provide new biocontrol resources for the sustainable management of fusarium head blight. A high-efficiency biocontrol strain was isolated from non-agricultural soil samples collected in Badong County, Enshi, Hubei Province, using the dilution plate method with Fusarium graminearum as the indicator strain. The inhibitory effects of the isolate Glarea lozoyensis fermentation broth on mycelial growth and spore germination of F. graminearum were assessed,as well as its degradation rates on deoxynivalenol (DON) and zearalenone (ZEN) in vitro. The strain was identified through morphological characteristics and 18S rRNA gene sequence analysis. Further experiments were conducted to assess control efficacy in field plots. The results showed that a 10-fold dilution of its fermentation broth inhibited F. graminearum mycelial growth and spore germination by 91.98% and 100%, respectively, and achieved 100% degradation rates for DON and ZEN. Morphological characteristics and 18S rRNA gene analysis identified as Glarea lozoyensis (GL), designated GL-P1. Field trial results demonstrated that the fermentation broth of GL-P1 achieved a 71.36% control efficacy against Fusarium head blight, comparable to the fungicide tebuconazole. The above study indicates that Glarea lozoyensis P1 is a biocontrol isolate with promising field efficacy against Fusarium head blight, showing potential for further application and development.

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fusarium head blight / Fusarium graminearum / antimicrobial / Glarea lozoyensis P1 / mycelial inhibition / detoxifying mycotoxins

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GUO Haozhen , ZHOU Huazhong , DENG Chunlin , et al . Isolation and Identification of Glarea lozoyensis P1 and Antagonistic and Detoxification Activity Against Fusarium graminearum[J]. Chinese Agricultural Science Bulletin. 2025, 41(12): 107-112 https://doi.org/10.11924/j.issn.1000-6850.casb2024-0431

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