Effects of Drought-rehydration on Physiological Indexes and Drought-related Gene Expression of ‘YY021’ Seedlings

LI Lihua; XUE Bingjie; WANG Yale; LI Xuejun; SUN Huan; SUN Jiping

doi:10.11924/j.issn.1000-6850.casb2024-0436

PDF(1458 KB)

Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (8) : 57-62. DOI: 10.11924/j.issn.1000-6850.casb2024-0436

Effects of Drought-rehydration on Physiological Indexes and Drought-related Gene Expression of ‘YY021’ Seedlings

LI Lihua ¹ ,
XUE Bingjie ² ,
WANG Yale ² ,
LI Xuejun ² ,
SUN Huan ² ,
SUN Jiping ²

Author information +

History +

Abstract

‘YY021’ is a hybrid tobacco variety selected for dryland tobacco growing areas. In order to identify the drought tolerance of ‘YY021’, ‘Zhongyan 100’ was used as the control variety, and the drought-rehydration experiment of floating seedling was arranged to investigate wilting index and water content, observe the changes of active oxygen, and measure the content of malonaldehyde, proline and the expression of drought-related gene by qRT-PCR. The results showed that with the prolongation of drought time, the leaf water content of ‘Zhongyan 100’ and ‘YY021’ decreased and the wilting index increased. The content of malonaldehyde increased with the extension of drought time. The expression of osmotin gene decreased first and then increased from 0-7 d of drought. After 7 days of drought, compared with ‘Zhongyan 100’, the leaf moisture content of‘YY021’ increased by 4.31%, the wilting index of ‘YY021’ decreased by 3.97%, and the contents of malondialdehyde decreased by 9.21%, and the contents of proline increased by 84.71%. Compared with ‘Zhongyan 100’, ‘YY021’ had lower levels of reactive oxygen species and malondialdehyde, and the higher osmotin gene expression. The ERD gene responded more rapidly. The drought tolerance of ‘YY021’ was better than that of ‘Zhongyan 100’. Under drought stress, the damage of reactive oxygen species to membrane of ‘YY021’ was reduced by accumulating osmotic adjustment substance proline. ERD and osmotin genes may be candidate genes for drought tolerance of ‘YY021’.

Key words

flue-cured tobacco / ‘YY021’ / drought tolerance / drought-rehydration / water content / MDA / proline / gene expression

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LI Lihua , XUE Bingjie , WANG Yale , et al . Effects of Drought-rehydration on Physiological Indexes and Drought-related Gene Expression of ‘YY021’ Seedlings[J]. Chinese Agricultural Science Bulletin. 2025, 41(8): 57-62 https://doi.org/10.11924/j.issn.1000-6850.casb2024-0436

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis

[1]	马文广, 周义和, 刘相甫, 等. 我国烤烟品种的发展现状及对策展望[J]. 中国烟草学报, 2018, 24(1):116-122. Cited in this article [1]

[2]	孙计平, 吴照辉, 李雪君, 等. 21世纪中国烤烟种植区域及主栽品种变化分析[J]. 中国烟草科学, 2016, 37(3):86-92. Cited in this article [1]

[3]	ASCUIBE S, RUGGIERO C, N APOLITANO A. Effects of water volume and nitrogen fertilization on yield and quality traits of air-cured burley tobacco (Nicotiana tabacum L.)[J]. Contributions to tobacco research, 2010, 24(4):194-206. Cited in this article [1]

[4]	李雪君, 孙计平, 丁燕芳, 等. 烤烟新品种豫烟13号的选育及特征特性[J]. 中国烟草科学, 2017, 38(4):17-22. Cited in this article [2]

[5]	曹景林, 余君. 津巴布韦烤烟杂交种的利用与启示[J]. 烟草科技, 2017, 50(1):17-24. Cited in this article [1]

[6]	王思文. 干旱胁迫下AMEP蛋白对大豆幼苗抗旱性的调控效应[D]. 大庆: 黑龙江八一农垦大学,2023:4. Cited in this article [1]

[7]	丁燕芳, 孙计平, 尚晓颖, 等. 干旱对不同烤烟品种叶片细胞膜伤害和保护酶活性的影响[J]. 西南农业学报, 2015, 28(6):2746-2749. Cited in this article [1]

[8]	关扬扬, 郑淑心, 刘向阳, 等. NtCycB2基因表达对烟草抗旱性的影响[J]. 烟草科技, 2021, 54(3):1-8,16. Cited in this article [3]

[9]

吴丽芳, 魏晓梅, 蔡建. 白刺花幼苗抗旱与耐寒协同响应的生理生化评价[J]. 热带作物学报, 2023, 44(10):2051-2059.

https://doi.org/10.3969/j.issn.1000-2561.2023.10.016

Cited in this article [1] Abstract

为探讨白刺花幼苗抗旱与耐寒协同响应的生理生化适应机制，以白刺花实生苗为材料，采用盆栽自然干旱及人工控温方法，分析幼苗抗旱生理，再以人工低温处理对白刺花抗氧化酶系、细胞膜损伤以及渗透调节的影响。结果表明：自然干旱胁迫下，白刺花叶片相对含水量随着干旱时间的增长显著减小，复水后2 d又能恢复生长；适度的干旱有利于光合色素的积累，严重时光合色素含量反而降低；超氧化物歧化酶（superoxide dismutase，SOD）、过氧化物酶（peroxidase，POD）、过氧化氢酶（catalase，CAT）3种保护酶随着干旱时间的延长呈先升后降变化，SOD和POD活性8 d时达到峰值，分别为458.85、5.30 U/g，比对照增加142.53%、68.25%。丙二醛（malondialdehyde，MDA）含量随干旱增强呈缓慢上升的趋势，15 d达到高峰，峰值为6.20 mmol/g，比对照增加108.75%；渗透调节物质脯氨酸（proline，Pro）含量呈先增后降趋势，10 d高达459.67 mg/g，比对照增加226.45%；干旱胁迫与低温交叉处理，白刺花叶片光合色素含量明显减小，3种保护酶变化与干旱胁迫相似，呈先升后降变化；MDA含量随低温胁迫延长呈缓慢上升的趋势，Pro含量的变化呈上升趋势。不同胁迫时期3种保护酶对白刺花抗旱性与耐寒性主导作用不同，表现为相互协调的作用方式。逆境胁迫下白刺花通过提高抗氧化酶并积累渗透调节物质Pro以减轻活性氧对膜伤害；干旱胁迫可提高白刺花对低温的适应能力，这种交叉适应性可能与保护酶系和渗透调节能力的增强有关。

[10]	舒彦淇, 罗家骏, 贾傛吏, 等. 干旱胁迫对烟草两品种幼苗生理生化特性及NtDEGP5基因表达的影响[J]. 亚热带植物科学, 2023, 52(1):1-8. Cited in this article [2]

[11]	徐小艳, 姚新转, 吕立堂, 等. 烟草NtNAC1基因的克隆及其在烟草中的抗旱功能分析[J]. 植物生理学报, 2018, 54(6):1085-1094. Cited in this article [1]

[12]

陈力, 王靖, 邱晓, 等. 不同耐旱性紫花苜蓿干旱胁迫下生理响应和转录调控的差异研究[J]. 作物学报, 2023, 49(8):2122-2132.

https://doi.org/10.3724/SP.J.1006.2023.24205

Cited in this article [1] Abstract

苜蓿是最重要的豆科牧草, 常被种植在干旱/半干旱地区。为研究不同耐旱性紫花苜蓿应对干旱胁迫的调控机制, 本研究以中科1号紫花苜蓿(Medicago sativa ‘Zhongke 1’)为试验材料, 以三得利紫花苜蓿(M. sativa ‘Sanditi’)为对照, 采用自然干旱法进行处理, 比较干旱胁迫对其生长性状、光合作用、叶绿素含量、叶片相对含水量、渗透调节物质和抗氧化酶活性等指标的影响, 并使用转录组测序分析2个品种响应干旱基因的差异。结果表明, 干旱胁迫显著降低了2个紫花苜蓿品种的株高、地上地下生物量、叶片相对含水量、光合速率、蒸腾速率、气孔导度、胞间二氧化碳浓度和叶绿素含量; 与三得利相比, 干旱胁迫下中科1号紫花苜蓿丙二醛含量和电导率更低, 渗透调节物质积累和活性氧清除能力更强。中科1号和三得利分别有5308个和8053个响应干旱的基因; GO功能注释分析发现, 中科1号的346个干旱响应基因被显著富集在6个GO类别, 而三得利的1683个干旱响应基因被显著富集在29个GO类别; 此外, 我们还筛选到19个在中科1号中表达量显著高于三得利的抗旱关键基因。本研究发现紫花苜蓿可能通过上调抗旱基因SUS、P5CS、LEA、SOD、POD、PEPC、NCED等的表达, 提高渗透调节能力和抗氧化酶活性来维持相对较高的光合速率, 从而增强抗旱能力。研究结果为进一步挖掘紫花苜蓿响应干旱胁迫的候选基因和抗旱育种提供了理论依据。

[13]	任婧瑶, 王婧, 蒋春姬, 等. 花生种质苗期耐旱性鉴定与综合评价[J]. 沈阳农业大学学报, 2019, 50(6):722-727. Cited in this article [1]

[14]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2001. Cited in this article [1]

[15]	GECHEV T, PETROV V. Reactive oxygen species and abiotic stress in plants[J]. International journal of molecular sciences, 2020, 21(20):7433 Cited in this article [1]

[16]	庄晔, 葛嘉雪, 汪孝国, 等. 干旱胁迫后复水对烤烟生长及其生理特性的影响[J]. 中国烟草学报, 2022, 28(4):48-58. Cited in this article [1]

[17]	GUPTA A, RICO-MEDINA A, CANO-DELGADO A I. The physiology of plant responses to drought[J]. Science, 2020,368:266-269. Cited in this article [1]

[18]	徐冰沁. 基于转录组和蛋白质组学的谷子(Setaria italica L.)幼苗对干旱的响应机制研究[D]. 杨凌: 西北农林科技大学,2020:2. Cited in this article [1]

[19]	周炎, 丁松爽, 赵莉, 等. 烤烟品系LY1306在PEG干旱胁迫下的生理响应及转录组学分析[J]. 中国烟草学报, 2017, 23(6):36-44. Cited in this article [1]

[20]	CHEN Q H, YANG G W. Signal function studies of ROS, especially RBOH-dependent ROS, in plant growth, development and environmental stress[J]. Journal of plant growth regulation, 2020, 39(1):157-171. Cited in this article [1]

[21]	李冬, 王艳芳, 王丽君, 等. 基于实验室条件的干旱胁迫下4个烤烟品种(系)的生理响应及抗旱性评价[J]. 烟草科技, 2020, 53(6):9-19. Cited in this article [1]

[22]	岳岭. 玉米突变体m716的耐旱性鉴定及作用机制研究[D]. 郑州: 河南农业大学,2023:7 Cited in this article [1]

[23]	MUKARRAM M, CHOUDHARY S, KURJAK D, et al. Drought: sensing, signaling effects andtolerance in higher plants[J]. Physiologia plantarum, 2021, 172(2):1291-1300. Cited in this article [1]

[24]	GOEL D, SINGH A K, YADAV V, et al. Overexpression of osmotin gene confers tolerance to salt and drought stresses in transgenic tomato (Solanum lycopersicum L.)[J]. Protoplasma, 2010,245:133-141. Cited in this article [1]

[25]	李波, 曾琴, 赵丹, 等. 杜仲 EuERD16基因的克隆及功能分析[J]. 基因组学与应用生物学, 2023, 42(4):373-383. Cited in this article [1]

[26]	曹沙沙. 大豆GmERD15c基因的克隆及其功能分析[D]. 长春: 吉林农业大学,2022:1. Cited in this article [1]

[27]	史月芝, 张石凯, 杨春萍, 等. HaTRET1 基因的克隆及转基因烟草的耐旱性分析[J]. 分子植物育种, 2020, 18(21):7217-7224. Cited in this article [1]