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Inhibitory Effect of Slightly Acidic Hypochlorite Water on Spore Germination of Two Pathogenic Fungi of Strawberry and Its Effect on Strawberry Rhizosphere Soil Fungal Diversity
LIUZheng, ZHANGJingjing, ZHAONamula, XUPengbo, LIANHongli, XUXiaofeng, ZHANGLiqing
Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (12) : 113-122.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Inhibitory Effect of Slightly Acidic Hypochlorite Water on Spore Germination of Two Pathogenic Fungi of Strawberry and Its Effect on Strawberry Rhizosphere Soil Fungal Diversity
In order to explore the effect and application technology of slightly acidic hypochlorite water (SAHW) in strawberry disease control, the fungal inhibition tests (inhibitory effect on the spore germination) was used in this paper to study the antifungal activity of SAHW against the two disease pathogens (Fusarium oxysporum and Botrytis cinerea). Illumina Miseq sequencing technology was also used to analyze the fungal community structure and diversity of the strawberry rhizosphere soil during the planting, early blooming and full-fruiting stages of strawberry before and after SAHW treatment. The results showed that the germination rates of F. oxysporum and B. cinerea spores cultured in the 25 mg/kg SAHW treatment group were both lower than 10%, while the germination rate of the control group cultured by purified water was close to 100%. A total of 15 phyla, 45 classes, 96 orders, 220 families and 405 genera were identified by Illumina Miseq sequencing. Among them, Ascomycota, Mortierellomycota and Basidiomycota were the dominant fungi. At the genus classification level, the relative abundance of Aspergillus (2.21%-53.54%) and Trichocladium (4.76%-26.54%) was relatively high. The relative abundance of the phytopathogenic fungus Aspergillus reached the highest value during the flowering stage and then showed a decreasing trend in the treatment groups. In addition, Penicillium, Fusarium and Colletotrichum gradually accumulated in the strawberry rhizosphere soil treated with purified water and reached the highest level during the fruiting period, but remained at a low level in the strawberry rhizosphere soil treated with SAHW. This study preliminarily revealed the antifungal effect of SAHW and its impact on the diversity of strawberry rhizosphere fungi, providing a scientific basis for comprehensive prevention and control of strawberry diseases.
strawberry / Botrytis cinerea / Fusarium oxysporum / slightly acidic hypochlorite water / rhizosphere soil / fungal diversity / Illumina Miseq sequencing
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