To study the effects of mixed planting of barley varieties with different edge types and their combinations on forage yield, field experiments were conducted with four barley cultivars: two-rowed short-statured ‘Baopimai 26’ and ‘Baopimai 28’, and six-rowed medium-tall ‘Baodamai 20’ and ‘Baodamai 25’. Eight treatments were designed for both single planting and mixed planting comparative experiments. The results showed that, in single planting experiment, there was no significant difference in fresh grass yield between ‘Baodamai 25’ and ‘Baopimai 28’, which were significantly higher than those of ‘Baopimai 26’ and ‘Baodamai 20’. In mixed planting experiment, the combinations of the two-rowed cultivar ‘Baopimai 28’ and the six-rowed cultivars ‘Baodamai 25’ and ‘Baodamai 20’ produced the highest fresh forage yields, reaching 41.36 t/hm² and 31.19 t/hm², respectively. Compared with single planting of ‘Baopimai 28’, these mixed planting increased yields by 8.14% and 14.14%, respectively, and compared with single planting of ‘Baodamai 25’, they increased yields by 3.63% and 9.38%, respectively. Thus it is not recommended to mix the two-rowed cultivar ‘Baopimai 26’ with other cultivars. Moreover, the six-rowed cultivar ‘Baodamai 25’ performed better than ‘Baodamai 20’ in mixed cultivation.