In order to establish the method of aseptic germination and tissue culture of Maianthemum japonicum seeds, the seeds of M. japonicum were used as experimental materials, and the effects of exogenous hormone types, concentrations and soaking time on seed germination were investigated by orthogonal test. At the same time, the stems of Cervus elaphus were used for callus culture to obtain sterile seedlings. Finally, M. japonicum was introduced and cultivated in greenhouse. The results showed that 100 mg/L GA₃ and 500 mg/L ethephon could promote the growth process of M. japonicum seeds, and the effect of soaking for 48 h was better than that of soaking for 12 h and 24 h. In the treatment of callus of stem section of M. japonicum, after the sequential exchange of sodium hypochlorite solution disinfection and black material removal, the pollution rate of M. japonicum decreased, and there was no significant difference in browning rate. At a suitable temperature, M. japonicum can quickly break dormancy and grow. This study preliminarily clarified that there was no significant difference in the germination rate of M. japonicum seeds treated with different exogenous hormones, but it had a certain promoting effect on their germination, and the callus induction browning rate of M. japonicum stem segments was higher, which could provide reference for the rapid propagation of M. japonicum in the later stage.