Effect of Melatonin on Growth and Physiological Characteristics of Hybrid Pennisetum urpureum ‘Guimu-1’ Plantlets Under Drought Stress

YIN Minghua; HE Fanfan; ZHANG Jiaxin; HUANG Tianhui; LE Yun; ZHANG Qinrong

doi:10.11924/j.issn.1000-6850.casb2024-0783

PDF(1313 KB)

Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (19) : 132-142. DOI: 10.11924/j.issn.1000-6850.casb2024-0783

Effect of Melatonin on Growth and Physiological Characteristics of Hybrid Pennisetum urpureum ‘Guimu-1’ Plantlets Under Drought Stress

Author information +

History +

Abstract

To investigate the effects of melatonin on the growth and physiological characteristics of hybrid Pennisetum urpureum ‘Guimu-1’ under drought stress, and to explore the physiological mechanism of melatonin alleviating the growth inhibition of hybrid P. urpureum ‘Guimu-1’ under drought stress, hybrid P. urpureum ‘Guimu-1’ plantlets under 10% PEG6000 drought stress were treated with six concentrations of melatonin solution by plant tissue culture. The differences in the responses of morphological and physiological indicators to different melatonin concentrations were analyzed. The results showed that drought stress significantly inhibited the growth of hybrid P. urpureum ‘Guimu-1’ plantlets, while adding different concentrations of melatonin could alleviate the growth inhibition caused by drought stress, and the most significant relief effect was observed when the melatonin concentration was 100 mg/L. Compared with the treatment of drought stress alone, the addition of 100 mg/L melatonin under drought stress increased the plant height, root length, stem length, leaf length, total fresh mass, aboveground fresh mass, underground fresh mass, and root shoot ratio of hybrid P. urpureum ‘Guimu-1’ plantlets by 74.4%, 69.9%, 110.9%, 75.4%, 97.6%, 80.5%, 118.1% and 20.2%, respectively; increased the relative water content, chlorophyll a, chlorophyll b, carotenoids, total root length, total root surface area, average root diameter, total root volume, root vitality, ascorbic acid (AsA) content, reduced glutathione (GSH) content, soluble sugar (SS) content, soluble protein (SP) content, proline content, CAT activity, SOD activity, POD activity, IAA content, GA₃ content, ZR content, and ETH content by 40.2%, 69.2%, 117.3%, 141.3%, 87.8%, 38.3%, 62.5%, 35.9%, 59.2%, 113.6%, 80.8%, 72.2%, 52.5%, 25.7%, 48.7%, 82.5%, 27.5%, 38.5%, 49.8%, 55.4% and 9.1%, respectively; decreased the relative conductivity, ABA content, O₂^- content, H₂O₂ content and MDA content by 52.9%, 14.2%, 38.4%, 67.7%, 50.5%, respectively. Plant height, root length, stem length, leaf length, total fresh mass, aboveground fresh mass, underground fresh mass, root shoot ratio had significant or extremely significant positive correlations with relative water content, chlorophyll a, chlorophyll b, carotenoids, total root length, total root surface area, total root volume, root vitality, AsA, GSH, SS, SP, Pro, CAT, SOD, POD, IAA, GA₃, ZR, ETH, and had significant or extremely significant negative correlations with relative conductivity, H₂O₂, ABA. Therefore, melatonin could significantly promote the growth of hybrid P. urpureum ‘Guimu-1’ plantlets under drought stress, enhance their antioxidant capacity, increase their osmoregulatory substance content, inhibit their excessive production of reactive oxygen species, and reduce their membrane peroxidation level, thereby improve the drought tolerance of hybrid P. urpureum ‘Guimu-1’ plantlets.

Key words

melatonin / drought stress / hybrid Pennisetum urpureum ‘Guimu-1’ / plantlets / growth / physiological characteristics

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YIN Minghua , HE Fanfan , ZHANG Jiaxin , et al . Effect of Melatonin on Growth and Physiological Characteristics of Hybrid Pennisetum urpureum ‘Guimu-1’ Plantlets Under Drought Stress[J]. Chinese Agricultural Science Bulletin. 2025, 41(19): 132-142 https://doi.org/10.11924/j.issn.1000-6850.casb2024-0783

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis

[1]	杨膺白, 李菁华, 赵雅丽, 等. 桂牧一号杂交象草在人工瘤胃培养液中蛋白质及氨基酸的消化规律研究[J]. 黑龙江畜牧兽医, 2021(22):103-108. Cited in this article [1]

[2]	刘小飞, 孟可爱, 李科云. 氮肥对桂牧一号杂交象草叶绿素、含氮物含量和转氨酶活性的影响[J]. 西北农林科技大学学报(自然科学版), 2013, 41(12):43-48. Cited in this article [1]

[3]	王桢珍, 范体凤, 翁殊斐. 吸附类2种藤本植物对攀附面和干旱胁迫的生长响应[J]. 西南林业大学学报(自然科学), 2024, 44(5):9-17. Cited in this article [1]

[4]	王湘莹, 魏溧姣, 王晓明, 等. 干旱胁迫对紫叶紫薇容器苗生长及生理的影响[J]. 西南林业大学学报(自然科学), 2024, 44(5):1-8. Cited in this article [1]

[5]	CARDOSO A A, BILLON L M, BORGES A F, et al. New developments in understanding plant water transport under drought stress[J]. New phytologist, 2020, 227:1025-1027. Cited in this article [1]

[6]	刘亚西, 毛芮, 杨梦含, 等. 外源激素对干旱胁迫下黑麦草生理特性的影响及抗旱性评价[J]. 草业科学, 2024, 41(2):425-436. Cited in this article [1]

[7]	孟可爱, 刘小飞. 水分胁迫对桂牧一号杂交象草生长性能和产草量的影响[J]. 湖南畜牧兽医, 2020(5):41-43. Cited in this article [2]

[8]	胡瑜, 申乔萍, 徐倩, 等. 生长调节剂绿比多和浦绿对狗牙根耐荫性和草坪质量的影响[J]. 中国草地学报, 2022, 44(8):69-76. Cited in this article [1]

[9]

王晶, 伏兵哲, 李淑霞, 等. 外源褪黑素对干旱胁迫下沙芦草幼苗生长和生理特性的影响[J]. 应用生态学报, 2023, 34(11):2947-2957.

https://doi.org/10.13287/j.1001-9332.202311.004

Cited in this article [7] Abstract

为明确外源褪黑素对沙芦草干旱胁迫的缓解效应,以‘盐池’沙芦草为试材,采用聚乙二醇6000(PEG-6000)模拟干旱胁迫,研究沙芦草幼苗对干旱胁迫的响应以及外源添加不同浓度(0、1、10、50、100、150和200 mg·L^-1)褪黑素对干旱胁迫下沙芦草幼苗生长和生理特性的影响。结果表明: 干旱胁迫显著抑制沙芦草幼苗的生长,而外源添加不同浓度的褪黑素均能够缓解干旱胁迫引起的沙芦草幼苗生长抑制,并且当褪黑素浓度为100 mg·L^-1时缓解效果最显著。与单独干旱胁迫处理相比,在干旱胁迫下外源添加100 mg·L^-1褪黑素后沙芦草幼苗株高、地上干重和叶片相对含水量分别增加58.2%、121.2%和48.1%,叶绿素a、叶绿素b和类胡萝卜素含量分别增加48.7%、80.8%和38.3%,超氧化物歧化酶、过氧化物酶和根系活力分别增加12.6%、33.9%和39.1%,抗坏血酸和谷胱甘肽含量分别增加19.5%和18.3%,脯氨酸、可溶性糖和可溶性蛋白含量分别提高16.2%、32.6%和14.3%,而丙二醛、过氧化氢和超氧阴离子含量分别降低45.8%、65.8%和30.8%。综上,外源添加100 mg·L^-1褪黑素能够显著促进沙芦草幼苗生长,增强抗氧化能力,增加渗透调节物质含量,抑制干旱胁迫下沙芦草植株体内活性氧的过度产生,降低膜质过氧化水平,从而提高沙芦草幼苗的耐旱性。

[10]	SHAHID I, FAISAL H, MUJAHID H, et al. Melatonin supplementation alleviates drought stress in peach (Prunus persica) seedlings by improving photosynthesis, root morphological traits, and antioxidant defense system[J]. Acta physiologiae plantarum, 2024, 46:18. Cited in this article [5]

[11]	HAMZEH A, ZOHREH Z, ARNAO M B. Optimal concentration of melatonin enhances drought stress tolerance in fenugreek[J]. Acta physiologiae plantarum, 2024, 46:15. Cited in this article [1]

[12]	张亚楠, 张津铨, 王君. 外源褪黑素对干旱胁迫下多年生黑麦草幼苗生理特性的影响[J]. 黑龙江畜牧兽医, 2024(3):86-92. Cited in this article [2]

[13]

王慧, 王冬梅, 张泽洲, 等. 外源褪黑素对干旱胁迫下黑麦草和苜蓿抗氧化能力及养分吸收的影响[J]. 应用生态学报, 2022, 33(5):1311-1319.

https://doi.org/10.13287/j.1001-9332.202205.004

Cited in this article [1] Abstract

以黑麦草和苜蓿为对象,分别叶面喷施和根施100 μmol·L^-1的褪黑素溶液,在干旱胁迫下测定了生物量、丙二醛(MDA)含量、相对电导率、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性、养分含量(有机碳C、全氮N、全磷P)等指标,研究外源褪黑素对干旱胁迫下植物抗氧化能力及养分吸收的影响。结果表明: 干旱胁迫下,黑麦草和苜蓿的地上、地下生物量显著降低,外施褪黑素能够有效缓解干旱胁迫对黑麦草和苜蓿生长的抑制作用,叶面喷施和根施褪黑素使干旱胁迫下黑麦草的生物量分别增加14.5%和29.6%,苜蓿的生物量分别增加36.6%和49.1%。干旱胁迫下,黑麦草的SOD、POD活性和苜蓿的SOD活性显著降低,外施褪黑素显著提高黑麦草和苜蓿的SOD、POD、CAT活性,减少叶片中MDA的积累,使叶片相对电导率显著下降,抗氧化能力显著提高。干旱和外施褪黑素对黑麦草和苜蓿有机碳含量无显著影响。干旱胁迫下,黑麦草叶片和根中的N、P含量以及苜蓿根中的N含量降低,外施褪黑素提高黑麦草和苜蓿根和叶片中的N、P含量,这表明褪黑素对干旱胁迫下黑麦草和苜蓿的养分吸收有一定的调节作用。施用褪黑素不仅能改善植物的抗氧化能力,还能调节养分吸收以增强植物对干旱胁迫的适应性,而且叶面喷施褪黑素效果好于根施。

[14]

尉欣荣, 张智伟, 周雨, 等. 褪黑素对低温和干旱胁迫下多年生黑麦草幼苗生长和抗氧化系统的调节作用[J]. 草地学报, 2020, 28(5):1337-1345.

https://doi.org/10.11733/j.issn.1007-0435.2020.05.019

Cited in this article [1] Abstract

本研究以‘顶峰Ⅲ’为供试多年生黑麦草（Lolium perenne）品种，研究了低温和干旱胁迫下内源褪黑素（melatonin，MT）的响应，并采用叶面喷施不同浓度MT （0，20，100，1 000 μM）的方法，探讨了MT对低温和干旱胁迫下黑麦草幼苗生长和抗氧化防御系统的影响。结果表明：低温和干旱胁迫下内源MT水平均显著增加（P₂^·-，H₂O₂和丙二醛（malondialdehyde，MDA）含量显著增加；过氧化物酶（peroxidase，POD），过氧化氢酶（catalase，CAT），抗坏血酸过氧化物酶（ascorbate peroxidase，APX）和谷胱甘肽还原酶（glutathione reductase，GR）活性发生不同程度的改变；MT显著地降低了MDA和活性氧的积累，提高了CAT，APX和GR活性及脯氨酸含量。综上，低温和干旱胁迫下MT能够诱导植物体内抗氧化酶活性增强和脯氨酸积累，有效地清除活性氧，从而缓解了黑麦草的生长抑制，增强了其抗寒和抗旱性。

[15]

黄洁琼, 翟佳兴, 王颖, 等. 褪黑素对干旱胁迫下紫花苜蓿种子萌发及幼苗生理特性的影响[J]. 草地学报, 2024, 32(8):2575-2583.

https://doi.org/10.11733/j.issn.1007-0435.2024.08.025

Cited in this article [4] Abstract

为探究褪黑素预处理对紫花苜蓿(Medicago sativa L.)幼苗抗旱性的影响，本试验以‘中苜一号’紫花苜蓿为材料，设置4个聚乙二醇(Polyethylene glycol，PEG)浓度和5个褪黑素浓度进行发芽试验，分别筛选出最佳的PEG和褪黑素浓度来处理紫花苜蓿幼苗，观测处理前后紫花苜蓿幼苗的表型及生理指标。结果表明，50 μmol·L^-1和100 μmol·L^-1褪黑素预处理能有效提高10% PEG模拟干旱胁迫下紫花苜蓿种子的发芽率、幼苗鲜重及根长；50 μmol·L^-1褪黑素预处理显著提高了干旱胁迫下紫花苜蓿幼苗的叶片含水量、可溶性糖含量和脯氨酸含量，另外，电解质渗透率和丙二醛含量均显著降低，抗氧化酶合成相关基因的表达水平显著上调，抗氧化酶活性增强。本研究验证了适宜浓度的褪黑素处理能有效缓解干旱胁迫对紫花苜蓿幼苗造成的损伤，增强紫花苜蓿幼苗的抗旱性，为褪黑素在紫花苜蓿耐旱栽培中的合理使用提供了一定的参考。

[16]

张玲, 麻冬梅, 刘晓霞, 等. 根灌外源褪黑素对干旱胁迫下紫花苜蓿生理特性的影响研究[J]. 草地学报, 2024, 32(1):198-206.

https://doi.org/10.11733/j.issn.1007-0435.2024.01.021

Cited in this article [1] Abstract

为探究褪黑素对干旱胁迫下紫花苜蓿（Medicago sativa L.）生长的影响，本研究以‘中苜3号’紫花苜蓿为试验材料，设置正常供水和干旱2个水分梯度，采用盆栽试验根灌不同浓度褪黑素，分析不同条件下幼苗表型、生理生化指标的变化。结果表明：90 μmol·L^-1褪黑素处理能够显著提高紫花苜蓿幼苗株高、茎粗、相对含水量、相对叶绿素含量，此外，90 μmol·L^-1褪黑素处理显著增加干旱胁迫下紫花苜蓿幼苗叶片抗氧化酶活性及酶合成相关基因表达水平，并降低脯氨酸、可溶性糖含量及丙二醛、相对电导率、过氧化氢含量。综上所述，褪黑素提高干旱胁迫下紫花苜蓿幼苗抗氧化酶生物合成基因表达水平，增强抗氧化酶活性，清除过量活性氧，缓解膜损伤，提高幼苗抗旱性。

[17]	冯蕊, 周琪, 吴令上, 朱. PEG 6000模拟干旱胁迫对铁皮石斛幼苗生理和叶绿素荧光特性的影响[J]. 浙江农林大学学报, 2024, 41(1):132-144. Cited in this article [1]

[18]	梁玉, 李金凤, 崔键, 等. 炭化液对NaCl胁迫下高丹草种子萌发和幼苗生长的影响[J]. 植物资源与环境学报, 2024, 33(3):80-88. Cited in this article [3]

[19]	IMRAN M, KHAN A L, SHAHZAD R, et al. Exogenous melatonin induces drought stress tolerance by promoting plant growth and antioxidant defence system of soybean plants[J]. AoB plants, 2021, 13(4):plab026. Cited in this article [1]

[20]	EFIMOVA M V, SAVCHUK A L, HASAN J A K, et al. Physiological mechanisms of enhancing salt tolerance of oilseed rape plants with brassinosteroids[J]. Russian journal of plant physiology, 2014, 61:733-743. Cited in this article [1]

[21]	ARNON D I. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris[J]. Plant physiology, 1949, 24:1-15. Cited in this article [1]

[22]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006:59-60. Cited in this article [1]

[23]	胡晓雯, 黄海娇, 张雯雯, 等. 基于超高效液相色谱同时测定木本植物中12种内源激素的方法及应用[J]. 分析科学学报, 2024, 40(3):300-308. Cited in this article [1]

[24]	马仲炼, 周苓宇, 陈悦, 等. 褪黑素浸种对辣椒种子萌发及幼苗抗旱性的影响[J]. 中国瓜菜, 2024, 37(9):128-135. Cited in this article [1]

[25]	王璐媛, 刘淋茹, 袁鑫茹, 等. 干旱胁迫条件下外源褪黑素提高小麦穗花发育和穗粒数的机理[J]. 植物营养与肥料学报, 2024, 30(8):1477-1489. Cited in this article [1]

[26]	洪越, 周贵兰, 刘瑞, 等. 外源褪黑素对干旱胁迫下不同抗旱型玉米幼苗生理特性的影响[J]. 北京农学院学报, 2024, 39(3):39-46. Cited in this article [3]

[27]	熊海琳, 田小霞, 毛培春, 等. 褪黑素浸种对干旱胁迫下白三叶种子萌发和幼苗生长的影响[J]. 中国草地学报, 2024, 46(5):23-32. Cited in this article [1]

[28]	邹询, 周宇飞, 李广, 等. 外源褪黑素对干旱胁迫下高粱生理代谢的调节效应研究[J]. 山西农业大学学报(自然科学版), 2024, 44(4):24-31. Cited in this article [1]

[29]	魏茜雅, 林欣琪, 梁腊梅, 等. 褪黑素引发对干旱胁迫下辣椒种子萌发和幼苗生长的影响[J]. 中国农业科技导报, 2024, 26(4):46-57. Cited in this article [1]

[30]	WANG Q, YUE J, YAN J M. Research progress on maintaining chloroplast homeostasis under stress conditions: A review[J]. Acta biochimica et biophysica sinica, 2023, 55(2):173-182. Cited in this article [1]

[31]	杨艳, 肖斌. 干旱胁迫下外源褪黑素对王族海棠光合作用、ASA-GSH循环以及激素变化的影响[J]. 河南农业科学, 2024, 53(6):100-110. Cited in this article [3]

[32]

梁佳, 胡朝阳, 谢志明, 等. 外源褪黑素缓解甜高粱幼苗干旱胁迫的生理效应[J]. 草业学报, 2023, 32(7):206-215.

https://doi.org/10.11686/cyxb2022340

Cited in this article [1] Abstract

为探究外源褪黑素缓解甜高粱幼苗干旱胁迫可能的生理机制，以甜高粱品种‘大力士’为材料，采用盆栽试验方法，分析自然干旱条件下，叶面喷施褪黑素（100 μmol·L^-1）对甜高粱幼苗生长及生理特性的影响。结果表明，干旱胁迫导致植株地上部水分失衡，叶绿体结构受损，细胞产生氧化胁迫，显著抑制甜高粱幼苗的生长。喷施褪黑素可显著降低甜高粱叶片丙二醛（MDA）含量，积累更多的可溶性蛋白和脯氨酸，提高叶片含水量，使叶绿体基粒片层排列有序，光合色素含量升高；喷施褪黑素还能显著提高叶片过氧化物酶（POD）和硝酸还原酶活性。综合上述，喷施100 μmol·L^-1褪黑素能进一步激发干旱环境中甜高粱叶片抗氧化系统的保护作用，通过渗透调节，维持植株水分平衡，改善叶绿体结构，提高光合色素含量，协同增强氮代谢，促进植株生长，从而缓解干旱对甜高粱幼苗的伤害。

[33]	XIA H, NI Z Y, HU R P, et al. Melatonin alleviates drought stress by a non-enzymatic and enzymatic antioxidative system in kiwifruit seedlings[J]. International journal of molecular sciences, 2020, 21:852. Cited in this article [1]

[34]	ANTONIOU C, CHATZIMICHAIL G, XENOFONTOS R, et al. Melatonin systemically ameliorates drought stress-induced damage in Medicago sativa plants by modulating nitro-oxidative homeostasis and proline metabolism[J]. Journal of pineal research, 2017, 62:e12401. Cited in this article [1]

[35]

姜瑛, 张辉红, 魏畅, 等. 外源褪黑素对干旱胁迫下玉米幼苗根系发育及生理生化特性的影响[J]. 草业学报, 2023, 32(9):143-159.

https://doi.org/10.11686/cyxb2022480

Cited in this article [1] Abstract

为研究喷施外源褪黑素对玉米幼苗抗旱性的效果，探究褪黑素缓解干旱胁迫的作用机理。以玉米品种郑单985作为供试材料，采用水培试验，使用聚乙二醇（PEG-6000）模拟干旱胁迫，探究干旱胁迫下不同浓度（0、10、50、100 μmol·L^-1）褪黑素对玉米幼苗生长、根系构型及分级特征、光合特性、抗氧化能力和渗透调节物质含量等指标的影响，采用隶属函数值法对不同处理下玉米幼苗的抗旱性进行综合评价。结果表明：与不施褪黑素处理相比，喷施50 μmol·L^-1褪黑素效果最优，玉米幼苗的株高、地上部鲜重和干重、地下部鲜重和干重、茎耐受指数和根耐受指数均显著增加；玉米幼苗的总根长（RL）、根表面积（SA）、根体积（RV）和根尖数（RT）均显著增加，RL的Ⅰ级（RD 0~0.5 mm）、SA的Ⅰ和Ⅱ级（RD 0.5~1.0 mm）、RV的Ⅰ和Ⅱ级均显著提高，其中50 μmol·L^-1褪黑素处理中的根系Ⅰ级径级区间的RL和SA占该处理根系的总比例最高，达到82.40%和47.32%；地上部丙二醛（MDA）含量显著降低了20.17%；地上部可溶性蛋白含量增加至最大，地下部可溶性蛋白含量显著升高了49.14%；光合速率（P_n）和气孔导度（G_s）分别增加了85.83%和32.81%；地上部和地下部超氧化物歧化酶（SOD）活性、地上部过氧化物酶（POD）活性、地上部过氧化氢酶（CAT）活性和地上部抗坏血酸过氧化物酶（APX）活性均显著升高；干旱胁迫下4个处理的抗旱性强弱排序为50 μmol·L^-1（MT₅₀）>10 μmol·L^-1（MT₁₀）>100 μmol·L^-1（MT₁₀₀）>0 μmol·L^-1（MT₀），即MT₅₀处理的抗旱性最强。外源喷施50 μmol·Ｌ^-1褪黑素有效调节了玉米幼苗地上部和地下部的抗氧化酶活性和渗透调节能力，减少了MDA含量，提高了地上部的光合作用能力，调节了玉米幼苗地上部和地下部的可溶性蛋白含量、抗坏血酸（AsA）含量、谷胱甘肽（GSH）含量、SOD活性、POD活性、APX活性和CAT活性，提高了玉米幼苗地上部和地下部的生物量和伸长量，减轻了干旱胁迫对根系构型的影响，提高了玉米幼苗的抗旱性，为研究褪黑素缓解干旱胁迫的作用机理提供了理论依据。

[36]

HASANUZZAMAN

, RAIHAN

M R H

, SIDDIKA

, et al. Selenium and its nanoparticles modulate the metabolism of reactive oxygen species and morpho-physiology of wheat (Triticum aestivum L.) to combat oxidative stress under water deficit conditions[J]. BMC plant biology, 2024, 24:578.

Cited in this article [1]

[37]	SAMANTA S, SETH C S, ROYCHOUDHURY A. The molecular paradigm of reactive oxygen species (ROS) and reactive nitrogen species (RNS) with different phytohormone signaling pathways during drought stress in plants[J]. Plant physiology and biochemistry, 2024, 206:108259. Cited in this article [1]

[38]	YIN X D, LUO X, YANG F, et al. An effective transient expression system for gene function identification in Lotus japonicus[J]. Plant cell, tissue and organ culture, 2024, 156:57. Cited in this article [1]

[39]	ALHARBY H, FAHAD S. Melatonin application enhances biochar efficiency for drought tolerance in maize varieties: Modifications in physio-biochemical machinery[J]. Agronomy journal, 2020, 112:2826-2847. Cited in this article [1]

[40]	WANG J J, LI R F, LIU D Y, et al. Endogenous hormones improve lodging tolerance of maize (Zea mays L.) by regulating stalk structure under elevated temperature[J]. Journal of plant growth regulation, 2024, 43:445-457. Cited in this article [1]

[41]	CHEN L J, HU Y W, HUANG L, et al. Comparative transcriptome revealed the molecular responses of Aconitum carmichaelii Debx. to downy mildew at different stages of disease development[J]. BMC plant biology, 2024, 24:332. Cited in this article [1]

[42]	LI D, SHEN H T, WANG Y F, et al. Effects of exogenous melatonin on photosynthetic carbon assimilation and endogenous hormones in tobacco seedlings under drought stress[J]. Acta pratacultural science, 2021, 30:130. Cited in this article [1]

[43]	李冬, 申洪涛, 王艳芳, 等. 外源褪黑素对干旱胁迫下烟草幼苗光合碳同化和内源激素的影响[J]. 草地学报, 2021, 30(1):130-139. Cited in this article [1]

[44]	罗艳. 外源褪黑素缓解园林小菊“绚秋凝红”干旱胁迫的生理效应[D]. 银川: 宁夏大学, 2023. Cited in this article [1]

[45]	臧祎娜, 张德闪, 李海港, 等. 褪黑素调控根系生长和根际互作的机制研究进展[J]. 植物营养与肥料学报, 2019, 25(4):671-682. Cited in this article [1]