PDF(1292 KB)
Salt Tolerance Response and Comprehensive Evaluation of Nine Kinds of Fraxinus at Seedling Stage
YUANChenchen, YANLiping, LILi, ZHOUJian, SUNChao, WANGYinhua, LITianjiao
Chin Agric Sci Bull ›› 2025, Vol. 41 ›› Issue (34) : 23-29.
PDF(1292 KB)
Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
PDF(1292 KB)
Salt Tolerance Response and Comprehensive Evaluation of Nine Kinds of Fraxinus at Seedling Stage
To study the changes in growth traits and physiological responses of Fraxinus hybrid seedling under salt stress and understand the salt tolerance of different hybrid seedlings, seven kinds of Fraxinus hybrid seedlings and two new varieties of Fraxinus were used as test materials to study their physiological responses to salt stress (0‰, 4‰, 8‰, 12‰), and the salt tolerance of nine kinds of Fraxinus seedlings was comprehensively evaluated by membership function method. The results showed that with the increase of salt stress intensity, the growth of hybrid seedlings was inhibited. The plant height and ground diameter of ‘327’ and ‘325’ decreased less, and the effect of salt damage was not obvious, while the inhibition of salt stress on the growth of ‘Qingbi’ and ‘344’ was more obvious. Compared with other combinations, the conductivity and MDA content of ‘327’ and ‘325’ changed more smoothly under salt stress, the chlorophyll content decreased less, the SOD activity in leaves increased more, and the proline content and soluble sugar content increased more than other hybrid seedlings. The conductivity and MDA content of ‘Qingbi’ and ‘344’ increased significantly, the chlorophyll content decreased greatly, and the SOD enzyme activity, proline content and soluble sugar content in leaves increased less. It indicated that under salt stress, ‘327’ and ‘325’ had stronger antioxidant enzyme protection system, more complete cell membrane structure, lower membrane lipid peroxidation, lower chlorophyll loss, more photosynthetic accumulation of photosynthetic products, faster plant growth and higher salt tolerance, while ‘Qingbi’ and ‘344’ had poor salt tolerance. The salt tolerance of 9 kinds of Fraxinus seedlings was comprehensively evaluated by membership function method, and the order of salt tolerance was ‘327’>‘325’>‘Lula 6’>‘318’>‘317’>‘346’>‘342’>‘344’>‘Qingbi’.
Fraxinus / salt stress / physiological response / salt tolerance / comprehensive evaluation
| [1] |
孙波, 朱安宁, 姚荣江, 等. 潮土、红壤和盐碱地障碍消减技术与产能提升模式研究进展[J]. 土壤学报, 2023, 60(5):1231-1247.
|
| [2] |
|
| [3] |
|
| [4] |
杨升, 刘正祥, 张华新, 等. 3个树种苗期耐盐性综合评价及指标筛选[J]. 林业科学, 2013, 49(1):91-98.
|
| [5] |
王友平, 刘德玺, 孙明高, 等. 白蜡良种选育研究的现状与对策[J]. 山东林业科技, 2007(6):81-83.
|
| [6] |
姚燕, 张峰. 绒毛白蜡盐碱地造林技术[J]. 宁夏农林科技, 2012, 53(7):27-28.
|
| [7] |
王友平, 刘德玺, 孙明高, 等. 绒毛白蜡营养器官中Na+、K+、Cl-的分布[J]. 石河子大学学报(自然科学版), 2009, 27(1):27-29.
|
| [8] |
常越霞. 盐胁迫对金叶白蜡生理生化特性的影响[D]. 保定: 河北农业大学, 2014.
|
| [9] |
刘翠兰, 吴德军, 王开芳, 等. NaCl胁迫下白蜡杂交苗的生长与生理响应[J]. 中国农学通报, 2016, 32(28):16-21.
为了解白蜡4个杂交组合种子苗的耐盐性，以1年生白蜡杂交种子苗为试材，研究其对NaCl胁迫(0‰、4‰、8‰、12‰)的生理响应。研究结果表明：4个杂交组合中，6号杂交组合苗的耐盐性最好。随着NaCl胁迫强度的增加，各组合植株的生长指标逐渐下降，但是6号组合植株生长高度下降幅度最低；相对于其他组合，盐胁迫下6号杂交组合的电导率增加率最低，丙二醛含量最低，叶片POD、SOD酶活性显著高于其他组合，而且Pro、可溶性糖和叶绿素的含量增加幅度大与其他组合相比达极显著水平。说明在盐胁迫下，6号组合抗氧化酶保护系统显著提高，从而降低叶肉细胞受伤害程度，使得细胞膜结构完整性较好，膜脂质过氧化程度较低，叶绿素损失也降低，光合作用较强，积累光合产物较多，表现在植株生长相对较快，耐盐性较高。该研究为选育白蜡耐盐新品种提供了理论基础。
|
| [10] |
王因花, 燕丽萍, 吴德军, 等. 绒毛白蜡‘青碧’不同半同胞家系的耐盐性评价[J]. 中南林业科技大学学报, 2020, 40(3):30-38.
|
| [11] |
燕丽萍, 吴德军, 王因花, 等. 绒毛白蜡耐盐生理与解剖结构研究[J]. 中国农学通报, 2024, 40(2):16-24.
为选育绒毛白蜡耐盐品种提供依据，本研究以二年生长势一致的绒毛白蜡新品种‘金箭’、华雄’、‘盐蜡’、‘鲁蜡3号’为研究对象，用9‰NaCl溶液处理期28 d，并对其进行生理指标的测定，通过隶属函数法选择绒毛白蜡的耐盐品种和盐敏感型品种，并对其进行解剖结构的制片观察，为该树种的耐盐选育及利用提供依据。盐处理28 d由生理指标测定值综合分析得到4个品种耐盐性强弱的排序为：‘盐蜡’>‘鲁蜡3号’>‘金箭’>‘华雄’。选择盐敏感型的‘华雄’和耐盐性较强的‘盐蜡’2个品种进行解剖结构的观察和测定发现：盐处理之后2个品种叶厚度、栅栏组织厚度、角质层厚度、木质部厚度等显著增加，且‘盐蜡’的叶厚度、栅栏组织厚度、海绵组织厚度、木质部与韧皮部厚度比值显著高于‘华雄’；‘盐蜡’处理之后的茎的皮层厚度显著高于对照，且处理之后韧皮部厚度显著降低，木质部厚度和最大导管直径均显著高于‘华雄’，处理之后2个品种的髓直径都出现显著增大，髓面积也相应地增加；盐处理之后2个品种根的周皮厚度均显著增加，同时‘华雄’皮层厚度显著增加，且显著高于‘盐蜡’，处理之后‘华雄’的中柱直径/根直径比值显著降低，说明其抵抗盐胁迫的能力较差。可用解剖结构研究代替生理研究对绒毛白蜡等树种的耐盐机制进行探索。
|
| [12] |
刘翠兰, 王开芳, 吴德军, 等. 滨海盐碱胁迫下白蜡无性系生长及生理特性的响应[J]. 中国农学通报, 2022, 38(35):7-16.
为筛选白蜡耐盐速生优良无性系，在滨海盐碱环境胁迫下，以国内收集的42个白蜡种质资源无性系为研究对象，利用相关性和主成分分析法对42个无性系干鲜比、株高、地径以及超氧化物歧化酶(SOD)、细胞膜透性、叶绿素、过氧化物酶(POD) 、丙二醛(MDA)生理代谢等指标进行遗传变异分析和综合评价。结果表明：42个无性系8个指标之间存在一定程度的差异，其中过氧化物酶(POD)变异系数最大，为64.33%，变异幅度25.00~458.30 U/(g·min)；其次为丙二醛(MDA)，变异系数为49.52%，变幅为0.50~7.08 umol/kg；再次为叶绿素和地径，变异系数分别为18.92%和18.56%，变幅分别为1.46~4.26 mg/g和1.15~2.78 cm；相关性分析结果表明，滨海盐碱胁迫下苗木干鲜比、株高和地径与各生理指标之间的相关性均达显著或极显著相关，说明利用生长指标和生理指标对无性系耐盐性进行综合评价，能反映滨海盐碱胁迫后的响应；利用主成分分析法对42份白蜡种质资源无性系综合得分及排名得知，各无性系耐盐碱性由强到弱前10位依次为‘盐3’>‘LC13’>‘济20’>‘R7-2’>‘盐2’>‘环翠3号’>‘胶南11’>‘白蜡4-2’>‘白蜡2’>‘DT21’。因此，初步认为上述10个无性系具有显著速生性和耐盐碱性，可作为盐渍立地造林或抗逆育种的重要应用材料。
|
| [13] |
赵世杰, 刘华山, 董新纯. 植物生理实验指导[M]. 北京: 中国农业出版社,1997:152-154,161-163.
|
| [14] |
李合生. 植物生理实验指导[M]. 北京: 高等教育出版社,2003:134-137.
|
| [15] |
杨升, 张华新, 刘涛. 16个树种盐胁迫下的生长表现和生理特性[J]. 浙江农林大学学报, 2012, 29(5):744-754.
|
| [16] |
马剑, 刘贤德, 金铭, 等. NaCl胁迫对文冠果幼苗生长性状的影响[J]. 中南林业科技大学学报, 2018, 38(1):11-15.
|
| [17] |
倪建伟, 武香, 张华新, 等. 3种白刺耐盐性的对比分析[J]. 林业科学研究, 2012, 25(1):48-53.
|
| [18] |
倪细炉, 岳延峰, 田英, 等. 4种盐生植物抗盐能力的综合评价[J]. 中国农学通报, 2010, 26(6):138-141.
|
| [19] |
|
| [20] |
魏秀君, 殷云龙, 芦治国, 等. NaCl胁迫对5种绿化植物幼苗生长和生理指标的影响及耐盐性综合评价[J]. 植物资源与环境学报, 2011, 20(2):35-42.
|
| [21] |
Contents Summary 523 I. Introduction 523 II. Sensing salt stress 524 III. Ion homeostasis regulation 524 IV. Metabolite and cell activity responses to salt stress 527 V. Conclusions and perspectives 532 Acknowledgements 533 References 533 SUMMARY: Excess soluble salts in soil (saline soils) are harmful to most plants. Salt imposes osmotic, ionic, and secondary stresses on plants. Over the past two decades, many determinants of salt tolerance and their regulatory mechanisms have been identified and characterized using molecular genetics and genomics approaches. This review describes recent progress in deciphering the mechanisms controlling ion homeostasis, cell activity responses, and epigenetic regulation in plants under salt stress. Finally, we highlight research areas that require further research to reveal new determinants of salt tolerance in plants.© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
|
| [22] |
赵江涛, 李晓峰, 李航, 等. 可溶性糖在高等植物代谢调节中的生理作用[J]. 安徽农业科学, 2006, 34(24):6423-6425.
|
| [23] |
|
| [24] |
|
| [25] |
|
| [26] |
孙海菁, 王树凤, 陈益泰. 盐胁迫对6个树种的生长及生理指标的影响[J]. 林业科学研究, 2009, 22(3):315-324.
|
| [27] |
杨传宝, 孙超, 李善文, 等. 白杨派无性系苗期耐盐性综合评价及筛选[J]. 北京林业大学学报, 2017, 39(10):24-32.
|
| [28] |
于金平, 俞珊, 梁有旺, 等. NaCl胁迫对美国白蜡幼苗部分生理指标的影响[J]. 植物资源与环境学报, 2014, 23(1):110-112.
|
/
| 〈 |
|
〉 |
