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Effects of Reduced Aphid Feeding on Survival and Oviposition of Coelophora biplagiata
LIUZhiwei, HUOLizhi, SUNLonghua
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (6) : 201-209.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Effects of Reduced Aphid Feeding on Survival and Oviposition of Coelophora biplagiata
To investigate the effects of reduced aphid feeding on larval survival and adult oviposition of Coelophora biplagiata, controlled laboratory experiments were conducted using a rearing system where early-instar larvae were fed Aphis craccivora and late-instar larvae received artificial diet under five density conditions (5, 10, 15, 20, and 30 larvae/container), combined with graded aphid reduction (0%, 25%, 50%, 75%, and 100%) during adult stage. Results demonstrated: (1) In Group A (artificial diet introduced from 3rd instar larvae), the 3rd instar larvae survival rate under the feeding density of 30 larvae/container was 65.56%, significantly lower than other densities (P<0.05). 4th instar larvae survival rate reached 100% under the feeding density of 5 larvae/container. Pupal survival rates ranged 82%-94% without significant differences (P>0.05). Adult emergence rate decreased with increasing density, peaking at 80.00% under the feeding density of 5 larvae/container. (2) In Group B (artificial diet introduced from 4th instar larvae), 4th instar larvae survival rates remained 74%-87% without significant differences (P>0.05). The lowest pupal survival rate occurred under the feeding density of 30 larvae/container (79.53%). Adult emergence rates under the feeding densities of 5-15 larvae/container ranged 75%-87% with no significant differences (P>0.05). (3) Group C showed highest adult emergence rate. (4) Oviposition performance declined with increasing aphid reduction, achieving 80% of full-aphid diet productivity at 25% aphid reduction. Artificial diet-fed adults showed minimal egg production. No significant differences were observed in egg hatch rates or adult survival across feeding regimes (P>0.05). In conclusion, partial replacement of aphids with an artificial diet is feasible for both larval rearing and adult oviposition of C. biplagiata.
Coelophora biplagiata / aphid / artificial diet / survival rate / fecundity
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为明确饲养密度对多异瓢虫发育历期、成蛹率、成虫率及重量的影响,探索多异瓢虫的高效饲养技术,设置4个初龄幼虫饲养密度(A:100头/盒、B:200头/盒、C:300头/盒、D:400头/盒,饲养盒容积为1000 mL),观察统计多异瓢虫的各龄幼虫蜕皮、化蛹、羽化时间以及蛹和羽化成虫数量。研究结果表明,A、B、C、D各组多异瓢虫的幼虫和蛹的发育历期分别为(11.75±0.65) d、(12.06±0.77) d、(12.45±0.60) d和(12.70±0.21) d,化蛹数量分别是(85.25±4.87)头、(147.50±3.12)头、(193.75±21.40)头和(156.25±29.62)头,其中可收集蛹分别为(49.00±6.68)头、(74.25±12.06)头、(107.75±16.76)头和(103.75±15.78)头,羽化成虫数量分别是(75.25±6.73)头、(123.75±8.05)头、(160.25±27.22)头、(110.75±26.68)头,成虫体重分别是(7.39±0.13)mg/头、(7.32± 0.29)mg/头、(7.29±0.20) mg/头、(6.56±0.12) mg/头,畸形成虫数量分别是(5.25±1.65)头、(12.75±4.33)头、(19.50±5.24)头、(10.50±3.90)头。随着饲养密度的增加,多异瓢虫发育历期会逐渐延长,化蛹、羽化及畸形数量逐渐增加,成蛹率、羽化率和成虫体重逐渐下降。综上所述,多异瓢虫初龄幼虫300头/盒的饲养密度,其化蛹和羽化成虫数量较多,体重正常,为较优饲养密度。
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梁辉, 邓全, 刘国, 等. 密度对十斑大瓢虫不同虫态生存的影响[J]. 中国农学通报, 2025, 41(8):105-110.
为了明确不同容积-密度组合饲养对十斑大瓢虫各虫态发育历期、存活率及蛹和成虫重量的影响,在25℃、相对湿度60%~80%、光照周期10 L:14 D条件下,通过组合3种容器(A:1000 mL;B:1500 mL;C:2000 mL)和3种密度(15、20、25头),饲喂、记录、分析十斑大瓢虫各虫态发育历期、存活率以及蛹和成虫重量。研究结果表明,十斑大瓢虫的存活曲线随龄期增长呈“S”型下降趋势,各处理发育历期从长到短依次为C<sub>3</sub>>C<sub>1</sub>>A<sub>1</sub>=C<sub>2</sub>>A<sub>3</sub>>A<sub>2</sub>=B<sub>2</sub>>B<sub>1</sub>>B<sub>3</sub>,B组的平均发育时间最短。各处理1—2龄幼虫期和蛹期存活率较低,B<sub>2</sub>处理的成蛹率为76.67%,成虫率为73.33%,高于其他处理。各处理蛹期的重量从高到低依次为A<sub>1</sub>>C<sub>2</sub>>C<sub>3</sub>>B<sub>1</sub>>A<sub>3</sub>>C<sub>1</sub>>B<sub>3</sub>>A<sub>2</sub>>B<sub>2</sub>,成虫的重量从高到低依次为B<sub>1</sub>>B<sub>3</sub>>B<sub>2</sub>>C<sub>2</sub>>C<sub>3</sub>>C<sub>1</sub>>A<sub>2</sub>>A<sub>3</sub>>A<sub>1</sub>。综上所述,十斑大瓢虫较优饲养容器应选择B<sub>2</sub>处理,即1500 mL饲养密度为20头/盒最佳。本研究为十斑大瓢虫幼虫的高效繁育提供理论基础。
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吴钰薇. 异色瓢虫规模化饲养及其捕食甜菜夜蛾的潜能研究[D]. 吉林农业大学, 2023a.
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李超. 六斑月瓢虫人工饲料的研究[D]. 福州: 福建农林大学, 2016.
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陆秋成, 刘东阳, 王勇, 等. 不同胡萝卜素浓度及饲料制作方法对七星瓢虫幼虫的影响[J]. 中国农学通报, 2021, 37(35):82-87.
为明确不同胡萝卜素含量及人工饲料制作方法对七星瓢虫幼虫的影响。采用不同浓度的胡萝卜素及其他组分组成的不同状态人工饲料对七星瓢虫幼虫进行饲养,对七星瓢虫幼虫和蛹的发育历期、成蛹率、成虫数量及体重进行评估、分析。结果表明:以50 mg/kg浓度胡萝卜素及其他组分饲养的幼虫的成蛹率为75.00%±5.00%(n=20)、成虫率为71.75%±2.00%(n=20)、幼虫期为(11.68±0.10)天(n=20),显著高于其他浓度胡萝卜素饲养组;体重(0.0325±0.0007) g(n=20)则显著低于其他浓度饲养组,蛹期(3.70±0.11)天(n=20)与其他浓度饲养组相比均无显著性差异。干粉饲料加糖水的组合饲养的七星瓢虫幼虫的蛹期(3.80±0.06)天(n=20)和体重(0.0357±0.0006) g(n=20)与糊状饲料饲养组的蛹期(3.55±0.10)天(n=20)和体重(0.0345±0.0010) g(n=20)没有显著性差异,但干粉饲料加糖水的组合饲养的七星瓢虫幼虫的成蛹率57.50%±2.00%(n=20)和成虫率41.25%±5.00%(n=20)均显著高于糊状饲料饲养七星瓢虫幼虫的成蛹率18.00%±2.00%(n=20)和成虫率17.00%±2.00%(n=20),而幼虫期(9.93±0.17)天(n=20)则显著低于糊状饲料饲养七星瓢虫幼虫的幼虫期(11.68±0.16)天(n=20)。50 mg/kg浓度的胡萝卜素组成的人工饲料饲养七星瓢虫幼虫,其成虫率和成蛹率显著高于其他组合,干粉状人工饲料显著优于糊状人工饲料,研究明确了1组较优的七星瓢虫幼虫饲料及其制作方法。
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伍兴隆, 刘东阳, 王勇, 等. 不同种类花粉对七星瓢虫幼虫发育的影响[J]. 中国植保导刊, 2020, 40(12):10-14,22.
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陈心玲, 金社林, 孙元星, 等. 补充饲喂油菜花粉对异色瓢虫幼虫生长发育及成虫生殖的影响[J]. 中国生物防治学报, 2025, 41(4):788-795.
异色瓢虫是一种重要的捕食性天敌,但其在自然条件下会补充取食花粉。为探究油菜花粉在该瓢虫饲养中的应用潜力,本试验首先基于市售干花粉通过将其与不同质量蒸馏水混合,评估了不同含水量花粉(9.1%,23.1%,33.3%与41.2%)对幼虫生长发育的影响,以干花粉作对照(CK)。进一步探究了桃蚜不足时,补充饲喂花粉(与适宜质量的水混合)对幼虫生长发育及成虫生殖的影响。结果表明:取食含水量为23.1%的花粉后,异色瓢虫发育至4龄和蛹期的存活率(48.9%和37.8%)及雌成虫体重(18.9 mg)均显著高于取食其他花粉的处理,总发育时间(27.3 d)也明显缩短;桃蚜不足时,补充饲喂花粉(含水量为23.1%)的瓢虫各龄幼虫存活率、化蛹率(93.9%)、羽化率(84.4%)及成虫产卵量(231.7粒)和卵孵化率(34.8%)均显著高于仅饲喂蚜虫的处理,且其成虫产卵前期显著缩短(18.0 d)。结果表明,适宜含水量的油菜花粉(23.1%)最利于异色瓢虫幼虫生长发育,并能显著提高其在桃蚜不足条件下的存活率及成虫生殖力。试验结果为科学应用油菜花粉饲喂捕食性瓢虫提供了重要参考,也为田间适时栽种油菜涵养天敌奠定了一定理论基础。
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孙元星, 陈明娟, 李明凌, 等. 人工饲料添加花粉对异色瓢虫幼虫生长发育的影响[J]. 中国生物防治学报, 2023, 39(5):1058-1065.
高效人工饲料是规模化生产捕食性瓢虫的重要基础,但目前大多数非昆虫源人工饲料的饲养效果较差。植物花粉富含丰富的营养与非营养成分,是捕食性瓢虫在自然条件下的重要替代猎物。但目前鲜有将其作为人工饲料成分的研究报道。本研究在异色瓢虫基础人工饲料(对照)中补充添加三个不同浓度的玉米花粉与玫瑰花粉,以上述饲料饲喂幼虫,旨在评价花粉在改进人工饲料品质中的应用潜力及花粉类型与浓度的影响。结果表明:异色瓢虫幼虫取食花粉添加饲料发育至成虫的存活率(68.33%~83.33%)显著高于取食对照饲料(36.67%),但发育时间与新羽化成虫体质量无明显差异。花粉种类及添加浓度对幼虫生长具有明显影响,以高浓度玫瑰花粉(RS-H)与中浓度玉米花粉(MZ-M)添加饲料的饲养效果最好,发育至成虫的存活率分别高达83.33%与80.00%。本研究结果表明,添加花粉可明显提升人工饲料品质,RS-H与MZ-M在异色瓢虫幼虫室内规模化饲养中具有重要应用潜力。
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吴钰薇, 高鹏, 郑林浩, 等. 东方粘虫对异色瓢虫生长发育及繁殖的影响[J]. 中国生物防治学报, 2023b, 39(6):1295-1300.
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Soil nutrition management and predation by Cheilomenes sexmaculata (Coleoptera: Coccinellidae) impacts on Aphis glycines (Homoptera: Aphididae). Understanding how soil nutrient availability and natural enemies impacts on aphid populations are important for soybean aphid, Aphis glycines Matsumura (Homoptera: Aphididae), management. Studies were conducted to examine the effect of soil nutrient management and predaceous coccinellid beetle, Cheilomenes sexmaculata (Fabr.), (Coleoptera: Coccinellidae) on A. glycines population. The plot size of 300 m2 was set up into 12 plots consisting four treatments and three replications. The treatments were the combination of NPK (urea 100 kg ha-1 + SP-36 200 kg ha-1 + KCl 200 kg ha-1) levels, dolomite (4 ton ha-1), compost (10 ton ha-1), and chicken manure (10 ton ha-1). While, the predator’s potential of C. sexmaculata was measured both in the field and laboratory conditions. The results of the studies indicated that soil nutrition treatments had significant effects on the plant performances: leaf numbers; pod numbers; and plant height. In line with this result, there were significant effects of soil nutrient treatments on the amount of plant nutrient: C, N, P, K, C, Ca, and Mg. The positive correlation coefficient was observed between the aphid population and nitrogen content, while the significant negative correlation found on between aphid population and the calcium and magnesium content. Meanwhile, the mean number of soybean aphids (A. glycines) consumed by C. sexmaculata increased from 8.915 to 40.64 aphids, with an average 26.35 ± 3.16 aphids. The mean numbers of aphids consumed by predator were significantly influenced by prey densities. The exclosure study result showed that the densities of A. glycines in caged plants was higher than that in un-caged plants. In the no-cage treatments, natural enemies had complete access to soybean aphids and caused the total reduction (100%) of A. glycines density by the 8th wk sample period. These findings suggest that reducing the amount of synthetic chemical fertilizer NPK can be supplemented with natural organic materials such as composts and chicken manure and natural occurring predator, C. sexmaculata, is an important natural enemy of the soybean aphid.
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林会, 刘东阳, 王勇, 等. β-胡萝卜素及饲料形态对七星瓢虫成虫生物学的影响[J]. 贵州农业科学, 2022, 50(11):43-48.
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徐金兰, 刘虹伶, 余佳敏, 等. 保幼激素类似物ZR-515对七星瓢虫生物学特性的影响[J]. 湖南农业科学, 2023(4):25-30.
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刘东阳, 伍兴隆, 陈雨, 等. 不同种类花粉对七星瓢虫繁殖能力的影响[J]. 湖南农业科学, 2022(11):60-64.
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刘虹伶, 何蓉, 余佳敏, 等. 氯化胆碱对七星瓢虫成虫生物学特性的影响[J]. 中国农学通报, 2024, 40(33):150-156.
为了解氯化胆碱对七星瓢虫成虫生物学特性的影响,筛选饲喂七星瓢虫的人工饲料中较优氯化胆碱剂量,设置5组添加不同剂量氯化胆碱(A、B、C、D、E剂量分别为50、100、150、200、250 mg/100 g)的人工饲料,与豆蚜进行组合饲喂(1 d豆蚜+3 d饲料)七星瓢虫成虫,以不添加氯化胆碱的人工饲料饲养七星瓢虫成虫为对照组(CK),记录各处理七星瓢虫的产卵量、取食、交配、孵化、存活率。研究结果表明饲养20 d,A、B、C、D、E、CK平均产卵量分别为(286.30±35.14)、(342.4±26.79)、(242.65±25.38)、(241.90±32.24)、(240.45±24.34)、(230.00±23.00)粒/雌,B组产卵量显著高于其他组,其余组间没有显著性差异;各处理七星瓢虫取食频次、交配频次、成虫成活率及卵的孵化率都没有显著性差异。添加100 mg/100 g的氯化胆碱制作的人工饲料与豆蚜组合饲养显著提高了七星瓢虫20日龄的产卵量,对其余生物学指标没有影响。
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陈子敬, 陈云芳, 刘美钊, 等. 花粉蜜源对异色瓢虫生物学特性的影响[J]. 河北农业科学, 2024, 28(3):74-77,100.
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It is known that food has a double impact on females of predatory ladybirds: qualitative signal effect (the onset of oogenesis) and quantitative nutritional effect (the increase in oogenesis intensity). We compared the patterns of these effects by feeding Cheilomenes propinqua females on mixed diets: unlimited low-quality prey (eggs of the grain moth Sitotroga cerealella) and limited high-quality prey (the green peach aphid Myzus persicae: 0, 2, 10, and 50 aphids per day). About half of the females fed only on the grain moth eggs oviposited and their fecundity was very low. Daily consumption of 2 aphids increased the proportion of egg-laying females whereas only consumption of 10 aphids increased their mean fecundity. Thus, the threshold of the signal effect was lower than that of the nutritional effect. As applied to mass rearing, we conclude that the addition of high-quality prey to low-quality food causes a substantial increase in egg production, although the economic feasibility of this method is not clear. Regarding biological control of pests by preventing colonization, we conclude that the fecundity of C. propinqua females supplied with the grain moth eggs in the absence of aphids will be low but the appearance of pests will cause a proportional increase in the mean fecundity of ladybirds.
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张培旭, 何恒果, 雷敏, 等. 不同剂型人工饲料对七星瓢虫成虫生物学特性的影响[J]. 中国生物防治学报, 2024, 40(1):27-32.
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Environmental factors play a crucial role in influencing sexual selection in insects and the evolution of their mating systems. Although it has been reported that sexual selection in insects may change in response to varying environments, the reason for these changes remains poorly understood. Here, we focus on the mate selection process of a ladybird, Cryptolaemus montrouzieri, when experiencing low- and high-nutrition diet regimes both in its larval and adult stages. We found that female ladybirds preferred to mate with males reared under high-nutrition diet regimes, regardless of the nutritional conditions they experienced during their own larval stages, indicating that mate choice of female C. montrouzieri is non-random and phenotype-dependent. Such mate choice may depend on visual cues (body or genitalia size) and/or chemical cues (pheromones). Further, females from high-nutrition larval diet regimes produced more eggs than those from low-nutrition larval diet regimes. In addition, diet regimes during adulthood also exerted strong effects on egg production. In summary, our study provides new insight into the mate choice of C. montrouzieri as affected by seasonal changes in resources and suggests that food availability may be a driving force in mate choice.
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