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Root Response Mechanism of Exogenous EBR Regulating Drought Resistance in Soybean Seedlings
XUFenfen, HANJinduo
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (8) : 53-59.
PDF(1477 KB)
Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
PDF(1477 KB)
Root Response Mechanism of Exogenous EBR Regulating Drought Resistance in Soybean Seedlings
To explore the effects of exogenous 2,4-Epibrassinolide (EBR) on root morphology and physiology of soybean seedlings under the drought stress, using ' Liaoxian No.1' as the material, the effects of 0.5-2.0 mg/L EBR treatment on root morphology, protective enzyme activity, reactive oxygen species accumulation, bleeding intensity and endogenous hormones (ABA, CTK ) of soybean seedlings under drought stress were studied by using pot water control method. The results showed that compared with CK, the drought stress treatment extremely significantly reduced the main root length, root surface area, root volume, main root diameter and root dry weight of soybean seedlings (P<0.01). Foliar spraying of 1.0-1.5 mg/L EBR could significantly alleviate the inhibition of root growth of soybean seedlings by drought stress. Among them, the main root length, root surface area, root volume, main root diameter and root dry weight of soybean seedlings treated with 1.0 mg/L EBR increased by 46.6%, 30.6%, 34.9%, 43.8% and 40.0% respectively. And all the above indicators were significantly higher than those of simple drought stress treatment. Foliar spraying 0.5-1.5 mg/L EBR significantly increased the activities of protective enzymes such as superoxidedismutase (SOD), peroxidase (POD), and catalase (CAT) in the root tips of soybean seedlings under drought stress (P<0.01), and significantly reduced the superoxide anion radical (O2-·) production rate and hydrogen peroxide (H2O2) content in the root tips of soybean seedlings under drought stress (P<0.01), reduced the ABA content in the root wound fluid to varying degrees, and increased the CTK content and root bleeding intensity. Soybean seedlings treated with 1.0 mg/L EBR had the highest protective enzyme activity in the root tip, bleeding intensity and CTK content, while the O2-· production rate, H2O2 content and ABA content were the lowest. Compared with the simple drought treatment, the SOD activity, POD activity and CAT activity in the root tips of soybean seedlings treated with 1.0 mg/L EBR increased by 14.0%, 17.2% and 50.6% respectively, the O2-· roduction rate and H2O2 content decreased by 33.0% and 17.8% respectively. Therefore, exogenous EBR alleviated the soybean drought stress by enhancing the root bleeding intensity and root tip protection enzyme activities of soybean seedlings under drought stress, increasing the CTK content, reducing the ABA content and inhibiting the active oxygen accumulation. Treatment with 1.0 mg/L EBR had the best effects on inducing drought resistance in soybean seedlings.
soybean (Glycine max L.) / root growth / protective enzyme activity / reactive oxygen species accumulation / bleeding intensity / hormone content
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【目的】水稻秧苗良好的根系盘结力，利于提高机插效率和质量。探明2,4-表油菜素内酯(2,4-epibrassinolide, EBR)在稻草生物炭育秧基质中对水稻根系生长的影响及作用机制具有重要意义。【方法】采用以300℃下制备的稻草生物炭为主的育秧基质，以甬优538为试验材料，分析了不同浓度EBR(0, 0.5, 1.0, 1.5, 2.0 mg/kg)对生物炭基质育秧水稻秧苗根系生长及生理特性的影响。【结果】在生物炭基质中加入EBR后水稻秧苗的根系盘结力增强了4.13%~22.46%，根系活力提高了0.93~1.65倍，根冠比增加了2.20%~14.10%，极大促进根系的生长。基质中添加EBR显著提高水稻秧苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化保护酶的活性，降低丙二醛(MDA)、过氧化氢(H<sub>2</sub>O<sub>2</sub>)的含量，增加秧苗的可溶性糖和可溶性蛋白含量。施用EBR后水稻根系和叶片中OsCu/Zn-SOD1和OsCu/Zn-SOD2、OsCAT1和OsCAT2四个基因的表达水平显著高于对照。【结论】生物炭育秧基质中增施EBR能够提高水稻秧苗抗氧化系统酶活性和相关基因表达，降低植株内MDA和H<sub>2</sub>O<sub>2</sub>的含量，改善水稻秧苗根系的生长和盘结能力，促进健壮秧苗的形成。基施EBR最适宜的浓度为1.0~1.5 mg/kg。
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