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Research Progress on Tissue Culture and Genetic Transformation in Chinese Chive
SUNYaling, ZHANGMingjun, LIUQinggang, SHURui, WANGZhenbao, FUZaiqiu, HUOYumeng
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (2) : 71-77.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Research Progress on Tissue Culture and Genetic Transformation in Chinese Chive
To issues of the lag in biological breeding of Chinese chives (Allium tuberosum) and the difficulty in achieving breakthroughs in functional research on many superior genes, there is an urgent need to clarify the current status and future directions of tissue culture and genetic transformation research as the key technological foundation. This paper reviews the domestic and international research achievements over three decades in the field, focusing on key aspects such as explant selection, sterilization optimization, medium screening, plant growth regulator formulation, rooting culture, acclimatization and transplanting, and genetic transformation conditions. It is found that: (1) the current tissue culture of Allium tuberosum remains in the primary stage of biological breeding. The explants are mainly flower axis and root tip, MS medium is commonly used basic medium, and the hormone ratio is mainly ' 6-BA + NAA ' combination. (2) The somatic embryo induction system is weak, the proliferation coefficient is low, the genetic transformation efficiency is insufficient, and there are problems such as non-standard terminology and industrial application faults. (3) Agrobacterium-mediated transformation is currently the main method, but has not yet bred transgenic varieties. In summary, non-standard terminology, imperfect regeneration system and low genetic transformation efficiency are the core bottlenecks restricting the molecular breeding of Allium tuberosum. The authors propose that it is necessary to standardize professional terminology, optimize explant-specific culture conditions by variety, clarify regeneration pathways and summarize the best system, optimize the genetic transformation system, strengthen somatic embryo and genetic transformation synergistic breeding, directionally introduce stress-tolerant and high-quality genes, and surmount the "bottleneck" problem of Allium tuberosum molecular breeding through synergistic innovation of multiple technologies to provide support for technological industrialization.
Chinese chives / tissue culture / regeneration system / genetic transformation / synergistic breeding
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