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Structural Characteristics of Rhizosphere Soil Microbial Communities in Wild Dendrobium denneanum with Different Growth Years
PENGCuixian, WANGCan, YANGYuling, LILing, LIGuilin, SUNHongwei, TAOYonghong
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (7) : 130-140.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Structural Characteristics of Rhizosphere Soil Microbial Communities in Wild Dendrobium denneanum with Different Growth Years
The paper aims to address the current issues of growth age and rhizosphere soil community structure of wild Dendrobium denneanum. To explore the microbial community structure of rhizosphere soil of D. denneanum with different growth years, and the correlation between microbial community and effective components of medicinal materials and soil nutrients, four soil samples were set up, including the control group of untreated soil without D. denneanum (XCK), and the rhizosphere soil of D. denneanum grown for 2 years (A2), 3 years (A3), and 4 years (A4). High-throughput sequencing was used to analyze the microbial community structure, while the contents of polysaccharides, phenols, extracts of D. denneanum and soil nutrients were determined. The results showed that with the extension of D. denneanum growth years, the richness and diversity of rhizosphere soil bacteria first decreased and then increased, whereas those of fungi first increased and then decreased. At the phylum level of bacteria, Proteobacteria had the highest relative abundance, with the proportion in A3 reaching 59.3%, which was the highest among all groups; followed by Bacteroidota which was most abundant in A4 (42.6%), and Acidobacteriota which was most abundant in XCK (11.9%). For fungi, Mortierellomycota was the dominant phylum, with the highest proportion in A2 (58.1%); Ascomycota was most abundant in XCK (52.9%); and Basidiomycota had the highest relative abundance in A3 (17.3%). Correlation analysis indicated that soil organic matter, humus, total phosphorus, total nitrogen, and calcium were significantly correlated with the rhizosphere soil microbial community of D. denneanum. Meanwhile, fungal genera such as Mortierella, Trichoderma, Ilyonectria were significantly positively correlated with some effective components of D. denneanum. In conclusion, the growth years of D. denneanum had a significant impact on the species composition and diversity of its rhizosphere soil microbial community.
Dendrobium denneanum / growth years / rhizosphere microorganisms / community structure / soil nutrients / active ingredients
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