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Identification and Mitochondrial Genome Analysis of a New Stored Pest of Sweetpotato: Paralipsa gularis (Zeller)
TANGFajiang, ZHOUShixu, HUANGLifei, WANGZhangying, ZOUHongda, KEJie, CHENXinliang, ZHANGShaorun
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (9) : 197-206.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Identification and Mitochondrial Genome Analysis of a New Stored Pest of Sweetpotato: Paralipsa gularis (Zeller)
This study aims to provide basic information on the new storage pest of sweet potatoes, Paralipsa gularis (Zeller), clarifying its phylogenetic relationship within the order Lepidoptera. This lays the basic for the identification and control of this pest. The author identified the morphological characteristics and cox1 gene of the pest, and utilized Illumina MiSeq sequencing technology to sequence its mitochondrial genome. The structural features and base composition were analyzed, and a phylogenetic tree was constructed using the maximum likelihood method (ML) based on the complete mitochondrial genomic sequences of closely related species to analyze the phylogenetic relationship with related species. The results indicate that the new sweetpotato storage pest is identified as P. gularis (Zeller), with a complete mitochondrial genome length of 15393 bp, an A+T content of 79.67%, and comprising 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes, totaling 37 genes, along with one non-coding control region. Among the 13 protein-coding genes, the start codon of the cox1 gene is CGA, while the start codons of the other genes are typical ATN. The termination codons for the nad2, cox1, cox2, and nad4 genes are incomplete stop codons (T--), whereas the termination codons for the other protein-coding genes are typical TAA or TAG. Of the 22 tRNA, only trnS1-GCT lacks the DHU arm, while the others can form typical cloverleaf structures. Phylogenetic analysis reveals that P. gularis (sweetpotato) is a member of the family Pyralidae (subfamily Galleriinae, superfamily Pyraloidea), closely related to Corcyra cephalonica (Stainton). This study is the first to report that P. gularis (Zeller) damages sweetpotato storage roots, and its distinct morphological characteristics and mitochondrial genomic sequence are clarified, which provide fundamental data for the identification, control, and research of this pest.
sweetpotato / pest / Paralipsa gularis (Zeller) / morphological characteristics / mitochondrial genome
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