Structure of Carbon Sequestration Bacterial Community and Screening of Carbon Sequestration Microorganisms in Rhizosphere Soil of Wine Grapes

YANG Siqi; XUE Tingting; ZHAO Xinru; GAO Lei; CAO Lingxiao; ZHAO Zhipeng; ZHANG Liang

doi:10.11924/j.issn.1000-6850.casb2025-0702

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Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (5) : 117-127. DOI: 10.11924/j.issn.1000-6850.casb2025-0702

Structure of Carbon Sequestration Bacterial Community and Screening of Carbon Sequestration Microorganisms in Rhizosphere Soil of Wine Grapes

YANG Siqi ¹ ,
XUE Tingting ¹^,²^,³ ,
ZHAO Xinru ¹ ,
GAO Lei ¹ ,
CAO Lingxiao ¹ ,
ZHAO Zhipeng ¹ ,
ZHANG Liang ¹^,²^,³

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Abstract

This research aims to investigate carbon sequestration microorganisms in vineyard soils and their characteristics of carbon source utilization, in order to provide a basis for enhancing soil organic matter in vineyard systems. Using the rhizosphere soil of 'Cabernet Sauvignon' grapevines as experimental material, this study employed high-throughput sequencing, screening with inorganic carbon-free media, and the Biology-ECO microplate method to analyze the community structure of carbon sequestration microorganisms and their efficiency in carbon source utilization. The dominant bacterial genera in the soils of the four 'Cabernet Sauvignon' vineyards were Bradyrhizobium, Nocardia, Mesorhizobium, Azospirillum, and Rubrivivax. Among them, the organically managed Bonabaifu Vineyard exhibited the highest richness of carbon sequestration microorganisms, with its Chao1 index being 29.70% higher than that of Hedon Vineyard and 46.10% higher than that of Gantu Vineyard. In addition, the genus Rubrivivax was identified as the key functional genus for carbon sequestration, accounting for 17.24%. The three isolated strains of carbon sequestration microorganisms all showed a preference for carbohydrate and amino acid carbon sources. This study confirmed that the carbon sequestration microorganisms in vineyards possessed relatively high species richness, and their community structures differed significantly among different vineyards. Organic and precision management practices can effectively enhance the abundance and functionality of these communities.

Key words

vineyard / rhizosphere soil / soil carbon sequestration microorganisms / carbon source utilization / Biology-ECO

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YANG Siqi , XUE Tingting , ZHAO Xinru , et al . Structure of Carbon Sequestration Bacterial Community and Screening of Carbon Sequestration Microorganisms in Rhizosphere Soil of Wine Grapes[J]. Chinese Agricultural Science Bulletin. 2026, 42(5): 117-127 https://doi.org/10.11924/j.issn.1000-6850.casb2025-0702

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