To investigate the effects of microbial inoculants on the yield of fresh edible broad beans and their control efficacy against root rot, three cultivars, ‘Yudou 3’, ‘Yundou 2883’ and ‘Yundouzao16’, were used as test materials. A three-factor orthogonal experiment was designed with the L₉(3⁴) orthogonal array, and three levels were set for each factor (cultivar, application frequency of microbial inoculant, and application rate of microbial inoculant). The experiment consisted of 9 treatments with 3 replications, totaling 27 plots, to examine the impacts of different application frequencies and rates of microbial inoculants on fresh pod yield and root rot control across different broad bean cultivars. The results showed that extremely significant differences existed in economic traits including pods per plant, branch number, fresh weight of 100 pods, fresh weight of 100 seeds and productivity per plant among the three cultivars (P<0.01). Among them, ‘Yundouzao 16’ achieved the best root rot control efficacy after inoculation, reaching 49.09%-50.35% relative to the non-inoculated control. Based on the comprehensive performance of all treatment combinations, the regime of ‘Yundouzao 16’ with 2 applications of microbial inoculant at a single rate of 84 kg/hm² delivered both favorable root rot control and the highest fresh pod yield among all tested treatments, and can serve as a reference combination for field production.