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Effects of Baking and Air-drying on Metabolite Conversion Results of Cigar Tobacco Leaves
CHENGYazhi, ZHANGShanshan, ZHONGXiaoli, LIAnjie, XIEHaibin, LYUXun, LIXueshan, DAIPeigang, ZHANGXingwei, ZHOUDongxin
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (12) : 177-185.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Effects of Baking and Air-drying on Metabolite Conversion Results of Cigar Tobacco Leaves
To screen the characteristic metabolites of cigar tobacco leaves and analyze the aroma differences of cigar tobacco leaves under baking and drying conditions, two cigar varieties, ‘QX103’ and ‘QX108’, were used as experimental materials. After treating the materials with drying and baking preparation methods, metabolomics detection was conducted to explore the differences in the transformation results of volatile and semi-volatile metabolites in tobacco leaves caused by different preparation methods. 172 semi-volatile metabolites were obtained through pre-column derivatization GC-MS technology. The OPLS-DA model was constructed, and 56 differential metabolites were screened out. Most of the differential metabolites were upregulated in baked cigars. By using the headspace solid-phase microextraction GC-MS technology, 65 volatile metabolites were detected in the cigars after drying, and 104 volatile metabolites were detected in the cigars after baking, among which 46 were shared by both. Differential metabolite pathways were mainly enriched in various types such as organic acids, sugars, amino acids, and esters. The effects of the two modulation methods on the metabolite transformation of cigar tobacco leaves were different. The quantity and abundance levels of volatile aroma and semi-volatile metabolites in the tobacco leaves after roasting were higher than those in the tobacco leaves after drying. It provides a theoretical basis for the design and construction of cigar drying rooms and the temperature and humidity control of the drying process in the future.
cigar / metabolomics / baking / air-drying / differential metabolites / path analysis
| [1] |
刘国顺. 烟草栽培学[M]. 北京: 中国农业出版社,2003:294-299.
|
| [2] |
刘博远, 赵松超, 李一凡, 等. 不同成熟度雪茄烟晾制过程碳水化合物及相关酶活性变化规律研究[J]. 中国农业科技导报, 2021, 23(4):192-201.
为了解不同成熟度晾制对雪茄烟叶品质的影响，以雪茄烟品种‘H382’为研究对象，采用大田试验方法，研究不同成熟度雪茄烟在晾制过程中碳水化合物含量及相关酶活性的变化，并对不同成熟度雪茄烟进行品质分析。结果表明，在晾制过程中适熟采收的烟叶失水速度和含水率适中，能促进内在物质的转化；雪茄烟适熟时采收，淀粉含量最低，淀粉降解速率快、降解较彻底，总糖、还原糖含量相对较高；淀粉酶和淀粉磷酸化酶活性均以适熟采收的烟叶最高；晾制后烟叶化学成分的主成分综合得分也以适熟采收的烟叶最高，化学品质较好。研究可为五指山雪茄烟叶采收提供理论基础。
|
| [3] |
徐世杰, 王洁, 王慧方, 等. 调制过程中不同温湿度条件对海南雪茄茄衣烟叶质量的影响[J]. 山东农业科学, 2016, 48(1):29-34.
|
| [4] |
宫长荣. 烟草调制学[M]. 北京: 中国农业出版社,2011:213-215.
|
| [5] |
黄维, 崔国民, 赵高坤. 不同成熟度烟叶在烘烤过程中主要挥发性香气成分的变化[J]. 中国农学通报, 2010, 26(24):149-152.
|
| [6] |
王岚, 王璐, 廖臻, 等. 气质联用仪对烤烟烘烤调制过程中烟叶主要致香成分变化的研究[J]. 分析测试学报, 2008, 27(S1):96-98.
|
| [7] |
赵会纳, 蔡凯, 雷波, 等. 烤烟中性致香物质在烘烤前后的差异分析[J]. 中国烟草科学, 2015, 36(2):8-13.
|
| [8] |
宫长荣, 王爱华, 王松峰. 烟叶烘烤过程中多酚类物质的变化及与化学成分的相关分析[J]. 中国农业科学, 2005, 38(11):2316-2320.
|
| [9] |
|
| [10] |
李卓, 张雄峰, 邱萍, 等. 挥发性化合物含量结合化学计量学用于建立江西烤烟的产地鉴别模型[J]. 南昌大学学报(理科版), 2019, 43(2):137-143.
|
| [11] |
李卓, 张雄峰, 孙瑞玲, 等. 顶空-气相色谱-质谱联用法结合化学计量学分析赣州烤烟样品中的挥发性化合物[J]. 广东农业科学, 2019, 46(4):130-137.
|
| [12] |
Although pre-column derivatization with n-propylamine and acetic anhydride combined with the gas chromatograph was a useful method for the analysis of monosaccharide composition, failure often occurs because of lack of detail information on the mechanism as well as the operating key point in derivatization process. In this study, the key points in the derivatization (lactonization time, the amount of n-propylamine and acetic anhydride) were investigated and optimized to improve the method. Under the optimal conditions, the derivatives of seven neutral monosaccharides and two uronic acids were simultaneouly obtained, after which they were well separated and detected by GC. It was also found that all derivatives of monosaccharides were stable even stored for 20days. The linearity, sensitivity, precision, reproducibility and recovery rate of the improved method were evaluated. Thereafter, five polysaccharide samples from different sources were analyzed to validate the improved method.Copyright © 2016 Elsevier Ltd. All rights reserved.
|
| [13] |
冯涛, 孙嘉卿, 张灿, 等. 结合GC-MS和GC-IMS分析不同处理方式下青豆的挥发性风味物质[J]. 粮油食品科技, 2021, 29(1):10-19.
|
| [14] |
孙嘉卿, 冯涛, 张灿, 等. 结合GC-MS和GC-IMS分析不同处理方式下玉米的挥发性风味物质[J]. 粮油食品科技, 2021, 29(1):1-9.
|
| [15] |
李亚会, 龚霄, 任芳, 等. 基于气相离子迁移谱分析不同贮藏条件下番荔枝的风味变化[J]. 食品工业科技, 2019, 40(18):263-266.
|
| [16] |
姬厚伟, 杨敬国, 王芳, 等. 静态顶空-气相色谱法在烟草行业中的应用进展[J]. 理化检验(化学分册), 2014, 50(9):1188-1192.
|
| [17] |
赖燕华, 肖明礼, 卢嘉健, 等. 基于烟丝动态顶空香气成分的卷烟品牌分析[J]. 中国烟草科学, 2017, 38(6):48-54.
|
| [18] |
田楠, 张春涛, 吴若昕, 等. 基于顶空气相色谱—离子迁移谱的不同品牌卷烟挥发性有机物指纹图谱研究[J]. 云南农业大学学报(自然科学), 2021, 36(3):465-471.
|
| [19] |
胡军, 余邦林, 姬凌波, 等. 基于SHS-GC-MS和GC-IMS的对两种雪茄烟挥发性香味成分的分析与研究[J]. 中国烟草学报, 2023, 29(2):111-121.
|
| [20] |
An improved pseudotargeted method using gas chromatography/mass spectrometry (GC/MS) was developed to investigate the metabolic profile of tobacco leaves from three planting regions (Yunnan, Guizhou, and Henan provinces). The analytical characteristics of the method with regard to reproducibility, precision, linearity, and stability were satisfactory for metabolic profiling study. Partial least-squares-discriminant analysis and hierarchical cluster analysis demonstrated that the metabolic profiles of tobacco from the Yunnan and Guizhou regions were different from that from the Henan province. The amino acid (e.g., phenylalanine, leucine, and tyrosine) and carbohydrate (e.g., fructose, trehalose, and sucrose) contents were the highest in Henan tobacco. The highest contents of organic acids (e.g., isocitrate, citrate, and fumarate) of the TCA cycle and antioxidants (e.g., quinate, chlorogenic acid, and ascorbate) were found in Guizhou tobacco. The correlation coefficients between metabolite content and climate factors (rainfall, sunshine, and temperature) demonstrated that drought facilitated the accumulation of sugars and amino acids. The content of TCA cycle intermediates could be influenced by multiple climate factors. This study demonstrates that the pseudotargeted method with GC/MS is suitable for the investigation of the metabolic profiling of tobacco leaves and the assessment of differential metabolite levels related to the growing regions.
|
| [21] |
郑庆霞, 刘萍萍, 陈霞, 等. 基于气质和液质联用技术的烟草鲜烟叶代谢组学分析流程[J]. 烟草科技, 2019, 52(6):59-71.
|
| [22] |
李娟, 任路静, 孙冠男, 等. 气相色谱-质谱联用技术及其在代谢组学中的应用[J]. 生物工程学报, 2013, 29(4):434-446.
|
| [23] |
毛德伦, 谢金伦, 郭亚东. 烟草中挥发性有机酸的气相色谱分析[J]. 云南大学学报, 1990, 12(2):156-158.
|
| [24] |
于瑞国, 王蕾, 孟广宇, 等. 烟草及烟草制品总挥发酸的测定——连续流动法[J]. 分析测试学报, 2005, 24(5):101-103.
|
| [25] |
葛少林, 佘世科, 徐迎波, 等. 烟草中挥发性与半挥发性有机酸的GC-MS法快速检测[J]. 香料香精化妆品, 2014, 143(2):31-40.
|
| [26] |
赵嘉幸, 陈黎, 任宗灿, 等. GC-MS/MS法测定烟草中的57种酯类香味成分[J]. 烟草科技, 2019, 52(12):39-49.
|
| [27] |
金辽, 刘银松, 林建枫, 等. 基于代谢组学解析‘翠碧一号’烟叶烘烤过程中多酚类代谢物动态变化[J]. 中国农学通报, 2024, 40(6):135-142.
为了解析烟叶烘烤过程中影响烟叶调制特性和香气品质的多酚类代谢物的动态变化，以‘翠碧一号’上部叶为材料，采集烘烤过程中5个关键节点15份样品，采用超高液相色谱串联质谱检测分析代谢物，通过K均值进行差异代谢物聚类，利用KEGG数据库进行注释和代谢通路分析。从15份样品中共鉴定出1069种代谢物，主要为黄酮类、酚酸类，脂质、生物碱、氨基酸等物质，其中多酚类为374种，达总数的34.99%。差异代谢物及其K均值聚类分析显示多酚类代谢物含量变化主要发生在变黄期至定色初期，代谢物富集在酪氨酸代谢，苯丙氨酸代谢、苯丙烷类和类黄酮生物合成通路中，差异代谢物在烟叶风味和品质相关的氢醌、绿原酸、山奈酚和芥子酸等物质的生物合成途径中显著富集。基于代谢组学解析了烟叶烘烤过程中的多酚类代谢物变化规律，为揭示烟草品质形成的物质基础和调控棕色化反应奠定基础。
|
| [28] |
赵会纳, 蔡凯, 秦嘉, 等. 烟叶烘烤前后主要代谢产物的差异分析[J]. 烟草科技, 2017, 50(9):61-67.
|
| [29] |
向东, 段淑辉, 丁松爽, 等. 不同成熟度雪茄烟叶晾制过程中颜色表征及主要化学成分变化特征[J]. 山东农业科学, 2022, 54(2):69-77.
|
| [30] |
陈爱国, 刘光亮, 周道金, 等. 龙岩鲜烟叶特征香气成分前体物代谢组学的通路分析[J]. 安徽农业大学学报, 2018, 45(3):545-550.
|
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