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Effects of Potassium Deficiency Stress on Growth and Antioxidant Characteristics in Lycium barbarum Seedlings
CHENGFanyi, ZHOUQixuan, LANLingkai, ZHANGRuoxi, YINYidi, HOUBohan, DUOLiangyu, GUJiageng, YAOYiduo, ZHANGZishuo, LANGXuping
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (7) : 92-96.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Effects of Potassium Deficiency Stress on Growth and Antioxidant Characteristics in Lycium barbarum Seedlings
To address the lack of scientific basis for potassium fertilizer management of Lycium barbarum var. formosanum, in order to reveal the effects of potassium deficiency stress on seedling growth and antioxidant characteristics, and to analyze the physiological mechanism of potassium nutrition, 40-day-old Lycium barbarum var. formosanum seedlings were used as materials, and two treatments of normal potassium (CK) and potassium deficiency were set up. The agronomic traits, biomass, chlorophyll content, soluble sugar content, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of seedlings were measured after 28 days. The results showed that potassium deficiency caused leaf yellowing or browning at the edges and tips, significantly reduced plant height and leaf spread, but significantly increased root length (P<0.001). The root-shoot ratio increased from 0.058 to 0.169, and the dry and fresh weight of the aboveground and underground parts decreased significantly. The contents of chlorophyll a, chlorophyll b and total chlorophyll decreased slightly, but the difference was not significant (P>0.05). The content of soluble sugar in plants was significantly increased (potassium deficiency 14.307 mg/(g·FW), CK 12.184 mg/(g·FW), P<0.001), the content of MDA was significantly increased (potassium deficiency 276.039 nmol/(g·FW), CK 207.099 nmol/(g·FW), P<0.001), and the activity of superoxide dismutase SOD was significantly decreased (potassium deficiency 171.412 U/g, CK 211.718 U/g, P<0.001). Potassium deficiency affects cell membrane stability and inhibits secondary metabolic processes by aggravating the accumulation of reactive oxygen species. The results showed that potassium deficiency stress inhibited the shoot growth and reduced biomass accumulation of Lycium barbarum var. formosanum by inducing oxidative stress and weakening antioxidant defense. ‘Suppressing the ground and promoting the underground’ was its stress adaptation strategy. This study provides a theoretical basis for the scientific potassium fertilizer management of Lycium barbarum var. formosanum. In the future, potassium concentration gradient can be set, the test cycle can be extended, and the molecular mechanism of its response to potassium deficiency stress can be deeply analyzed.
Lycium barbarum / potassium deficiency / growth / agronomic traits / antioxidant / malondialdehyde (MDA) / superoxide dismutase (SOD)
| [1] |
郭有燕, 刘宏军, 孔东升, 等. 干旱胁迫对黑果枸杞幼苗光合特性的影响[J]. 西北植物学报, 2016(1):124-130.
|
| [2] |
钱玥, 饶良懿. 盐碱胁迫对枸杞幼苗生长与叶绿素荧光特性的影响[J]. 森林与环境学报, 2022(3):271-278.
|
| [3] |
朱和. 水肥气热耦合对枸杞产量和品质的影响[D]. 银川: 宁夏大学, 2020.
|
| [4] |
南雄雄, 杨柳, 李文慧, 等. 缺素条件下枸杞植株生长及营养元素吸收利用互作效应[J]. 中国土壤与肥料, 2023(11):202-212.
|
| [5] |
胡明星, 陈清平, 王少东, 等. 枸杞营养失调及施肥措施概述[J]. 宁夏农林科技, 2011(2):91-92.
|
| [6] |
|
| [7] |
The calcium (Ca) concentration of plant shoot tissues varies systematically between angiosperm orders. The phylogenetic variation in the shoot concentration of other mineral nutrients has not yet been described at an ordinal level. The aims of this study were (1) to quantify the shoot mineral concentration of different angiosperm orders, (2) to partition the phylogenetic variation in shoot mineral concentration between and within orders, (3) to determine if the shoot concentration of different minerals are correlated across angiosperm species, and (4) to compare experimental data with published ecological survey data on 81 species sampled from their natural habitats. Species, selected pro rata from different angiosperm orders, were grown in a hydroponic system under a constant external nutrient regime. Shoots of 117 species were sampled during vegetative growth. Significant variation in shoot carbon (C), calcium (Ca), potassium (K), and magnesium (Mg) concentration occurred between angiosperm orders. There was no evidence for systematic differences in shoot phosphorus (P) or organic-nitrogen (N) concentration between orders. At a species level, there were strong positive correlations between shoot Ca and Mg concentration, between shoot P and organic-N concentration, and between shoot K concentration and shoot fresh weight:dry weight ratio. Shoot C and cation concentration correlated negatively at a species level. Species within the Poales and the Caryophyllales had distinct shoot mineralogies in both the designed experiment and in the ecological survey.
|
| [8] |
Nitrogen (N), potassium (K), and phosphorus (P) are essential macronutrients for plant growth and development, and their availability affects crop yield. Compared with N, the relatively low availability of K and P in soils limits crop production and thus threatens food security and agricultural sustainability. Improvement of plant nutrient utilization efficiency provides a potential route to overcome the effects of K and P deficiencies. Investigation of the molecular mechanisms underlying how plants sense, absorb, transport, and use K and P is an important prerequisite to improve crop nutrient utilization efficiency. In this review, we summarize current understanding of K and P transport and signaling in plants, mainly taking <i>Arabidopsis thaliana</i> and rice (<i>Oryza sativa</i>) as examples. We also discuss the mechanisms coordinating transport of N and K, as well as P and N.
|
| [9] |
|
| [10] |
赵平, 林克惠. 钾肥对农作物品质的影响[J]. 云南农业大学学报, 2001(1):56-59.
|
| [11] |
|
| [12] |
彭海欢, 翁晓燕, 徐红霞, 等. 缺钾胁迫对水稻光合特性及光合防御机制的影响[J]. 中国水稻科学, 2006(6):621-625.
以钾敏感型品种二九丰和钾钝感型品种原丰早为材料，研究了缺钾对水稻光合和叶绿素荧光特性的影响以及光合防御机制，并对缺钾条件下的光合防御机制进行了讨论。在缺钾条件下，两品种的净光合速率(Pn)随气孔导度(Gs)的下降而下降；光合作用的关键酶Rubisco含量降低，而抗氧化酶系统的关键酶SOD活性显著上升。进一步的叶绿素荧光特性研究表明，随着钾胁迫时间的延长，缺钾水稻叶片中的 Fv/Fm、ФPSⅡ、qP及ETR均显著下降，说明PSⅡ反应中心受到伤害。缺钾也降低了水稻叶片的光饱和点，PSⅡ吸收的光能超过了光合作用所能利用的光能。缺钾叶片NPQ显著上升，说明钾胁迫下的水稻叶片通过启动热耗散机制耗散过剩的激发能，以减轻因PSⅡ吸收过多光能而引起的光抑制和光氧化从而保护光合机构免受伤害。从品种间的差异来看，原丰早在缺钾条件下，由于仍有较强的光合能力和抗光抑制能力，因此对缺钾有更强的耐性。
|
| [13] |
|
| [14] |
王学奎, 黄见良. 植物生理生化实验原理与技术[M]. 北京: 高等教育出版社, 2015:1-324.
|
| [15] |
王桂芹. 硒和VitC对高氟所致氧化应激、DNA损伤及Bcl-2蛋白表达影响研究[D]. 广州: 广东药科大学, 2011.
|
| [16] |
邹琦. 植物生理生化实验指导[M]. 北京: 中国农业出版社, 2003:1-108.
|
| [17] |
Global climate change and associated adverse abiotic stress conditions, such as drought, salinity, heavy metals, waterlogging, extreme temperatures, oxygen deprivation, etc., greatly influence plant growth and development, ultimately affecting crop yield and quality, as well as agricultural sustainability in general. Plant cells produce oxygen radicals and their derivatives, so-called reactive oxygen species (ROS), during various processes associated with abiotic stress. Moreover, the generation of ROS is a fundamental process in higher plants and employs to transmit cellular signaling information in response to the changing environmental conditions. One of the most crucial consequences of abiotic stress is the disturbance of the equilibrium between the generation of ROS and antioxidant defense systems triggering the excessive accumulation of ROS and inducing oxidative stress in plants. Notably, the equilibrium between the detoxification and generation of ROS is maintained by both enzymatic and nonenzymatic antioxidant defense systems under harsh environmental stresses. Although this field of research has attracted massive interest, it largely remains unexplored, and our understanding of ROS signaling remains poorly understood. In this review, we have documented the recent advancement illustrating the harmful effects of ROS, antioxidant defense system involved in ROS detoxification under different abiotic stresses, and molecular cross-talk with other important signal molecules such as reactive nitrogen, sulfur, and carbonyl species. In addition, state-of-the-art molecular approaches of ROS-mediated improvement in plant antioxidant defense during the acclimation process against abiotic stresses have also been discussed.
|
| [18] |
孙蕊, 王锐, 高海娟, 等. 干旱胁迫对油莎豆抗氧化特性的影响[J]. 饲料研究, 2025(13):86-91.
|
| [19] |
|
| [20] |
周建刚, 丁少丽, 袁金友, 等. NaCl胁迫对苎麻叶片中SOD、POD及CAT活性的影响[J]. 武汉科技学院学报, 2007(4):35-37.
|
| [21] |
王忠, 顾蕴洁, 陈刚, 等. 植物生理学[M]. 北京: 科学技术文献出版社, 2006:61.
|
| [22] |
|
| [23] |
|
| [24] |
|
| [25] |
鲁一薇, 夏雪岩, 赵宇, 等. 缺钾胁迫下谷子转录组分析及相关基因挖掘[J]. 中国农业科技导报, 2024, 26(6):30-44.
|
/
| 〈 |
|
〉 |
