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Effect of Arbuscular Mycorrhizal Fungi (AMF) Inoculation on Resistance to Banded Leaf and Sheath Blight Disease of Maize
DONGChengyan, LIZhengnan, QIUWeihua, YUJing, CHENYiwei, ZHANGSiying, LIXiaolin, TANJing
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (21) : 23-36.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Effect of Arbuscular Mycorrhizal Fungi (AMF) Inoculation on Resistance to Banded Leaf and Sheath Blight Disease of Maize
This study aims to explore the impact of inoculating arbuscular mycorrhizal fungi (AMF) on the resistance of maize to banded leaf and sheath blight (BLSB), providing a basis for scientific prevention and control of BLSB and ensuring stable and high yield of maize. Taking the susceptible maize inbred line ‘B73’ as the test material, four treatments were set up, including single inoculation with the main pathogen of BLSB Rhizoctonia solani Kuhn (R), single inoculation with AMF (A), double inoculation (AR), and no inoculation (CK). The phenotype, physicochemical indicators, and rhizosphere soil microbial community of maize under different treatments were analyzed. The results showed that inoculating with AMF significantly promoted the growth of maize; under pathogen stress, the dry weight of the aboveground and underground parts in the AR treatment increased by 87.11% and 51.17% respectively, compared to the non-inoculated R treatment. Inoculating with AMF also significantly inhibited the spread of BLSB, the lesion length in the AR treatment decreased by 55.39% and 49.24% at 3 days and 5 days after inoculation respectively, compared to the non-inoculated R treatment. Both inoculation with AMF and pathogenic bacteria could significantly increase the content of JA and SA, as well as the activities of SOD, POD, PAL, and PPO in maize leaf sheaths and roots; double inoculation further enhanced these levels. Inoculating with AMF significantly enhanced the diversity of rhizosphere soil bacterial communities, leading to notable changes in community composition and function. The abundance of beneficial bacterial groups such as Bacteroidota, Actinobacteriota, and Acidobacteriota increased significantly. In summary, inoculating with AMF enhanced the resistance of maize to BLSB by promoting maize growth, improving relevant physicochemical indicators, and optimizing the rhizosphere soil microbial community. The research results can provide theoretical basis for developing AMF-based biological control strategies against maize BLSB.
maize / banded leaf and sheath blight (BLSB) / arbuscular mycorrhizal fungi (AMF) / Rhizoctonia solani Kuhn / physicochemical indicators / rhizosphere microbial community
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