Control Effect of Bio-organic Fertilizer Containing Pseudomonas fluorescens on Bacterial Wilt Disease in Facility Eggplants and Its Role in Yield Enhancement and Quality Improvement

HUANGJian

Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (14) : 166-171.

PDF(1833 KB)
Home Journals Chinese Agricultural Science Bulletin
Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

About  /  Aim & scope  /  Editorial board  /  Indexed  /  Contact  / 
PDF(1833 KB)
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (14) : 166-171. DOI: 10.11924/j.issn.1000-6850.casb2025-1039
Original article

Control Effect of Bio-organic Fertilizer Containing Pseudomonas fluorescens on Bacterial Wilt Disease in Facility Eggplants and Its Role in Yield Enhancement and Quality Improvement

Author information +
History +

Abstract

The study aimed to explore the control effect of bio-organic fertilizer containing Pseudomonas fluorescens on bacterial wilt of facility eggplant, and its influences on yield, quality and soil properties, so as to provide theoretical basis and technical support for scientific fertilization in continuous cropping of facility eggplant. Field experiment was conducted with three treatments, including conventional fertilization (CK), basal application of inactivated bio-organic fertilizer (K1), and basal application of bio-organic fertilizer containing Pseudomonas fluorescens (K2). The application effects of the bio-organic fertilizer were analyzed by determining eggplant growth indicators, fruit quality, incidence of bacterial wilt, and relevant soil properties. The results indicated that the K2 treatment significantly enhanced eggplant yield and quality, and exerted positive effects on soil enzyme activities and rhizosphere microbial biomass. Compared with CK, K2 treatment significantly increased fruit single weight, yield, soluble protein content, soluble sugar content, and vitamin C content by 10.58%, 7.79%, 31.47%, 25.85%, and 17.48% respectively, while reducing cellulose content by 65.52%. The activities of soil urease, catalase, sucrase, and acid phosphatase increased by 50.00%, 42.84%, 50.23%, and 30.04% respectively, and the populations of rhizosphere actinomycetes, fungi, and bacteria increased by 55.71%, 78.00%, and 15.57% respectively. The bio-organic fertilizer containing Pseudomonas fluorescens can achieve disease control, yield improvement and quality optimization of facility eggplant under continuous cropping by optimizing soil microecological environment and enhancing plant photosynthetic capacity, providing an effective pathway for resolving the obstacles associated with the continuous cropping of facility eggplant.

Key words

Pseudomonas fluorescens / bio-organic fertilizer / facility eggplant / application effect

Cite this article

Download Citations
HUANG Jian. Control Effect of Bio-organic Fertilizer Containing Pseudomonas fluorescens on Bacterial Wilt Disease in Facility Eggplants and Its Role in Yield Enhancement and Quality Improvement[J]. Chinese Agricultural Science Bulletin. 2026, 42(14): 166-171 https://doi.org/10.11924/j.issn.1000-6850.casb2025-1039

References

[1]
王菲, 李银科, 王正银, 等. 缓释复合肥对茄子产量和不同采果期品质的影响[J]. 土壤学报, 2015, 52(2):355-363.
[2]
卢家柱, 赵贵宾, 颉建明, 等. 不同施氮量对茄子产量、品质及肥料利用率的影响[J]. 华北农学报, 2016, 31(3):205-211.
为探究不同施氮量对茄子产量、品质及肥料利用率的影响,以陇优长茄为试验材料,采用盆栽试验,测定了不同施氮水平下(0,192,231,288,384kg/hm<sup>2</sup>),茄子干物质积累量、产量、营养品质及各器官养分含量。结果表明,茄子产量随着氮肥用量的增加先升高后降低,T2产量最高,为61920kg/hm<sup>2</sup>。氮肥用量(x)与茄子产量(y)之间存在显著的相关关系,数学模型为y=-0.570 9x<sup>2</sup>+290.03x+22915,R<sup>2</sup>=0.9899<sup>**</sup>。各施肥处理中,可溶性糖和可溶性蛋白含量均以T2最高,分别为3.433%和2.264mg/g;维生素C以T1最高,为70.610mg/100g;硝酸盐含量以T1最低,为311.952mg/kg。氮肥利用率随着施氮量的减少而升高,T1最高,为36.78%;磷、钾的利用率都以T2最高,分别为23.23%,33.82%。因此,适当减少氮肥用量(T2)能够使茄子产量达到最高的同时,还可显著改善茄子品质、提高肥料的利用率。
[3]
马兆红, 连勇, 方木壬, 等. 从生产市场需求谈我国茄子品种的变化趋势[J]. 中国蔬菜, 2017(9):1-6.
[4]
孙义, 居正英, 林玲, 等. 茄子黄萎病生防内生细菌29-12发酵条件的初步研究[J]. 江苏农业学报, 2008, 24(4):425-430.
[5]
陈廷钦. 土壤调理剂及应用进展[J]. 云南大学学报(自然科学版), 2011, 33(增刊1):338-342.
[6]
杨尚东, 李荣坦, 谭宏伟, 等. 长期单施化肥和有机无机配合条件下红壤蔗区土壤生物学性状及细菌多样性差异[J]. 植物营养与肥料学报, 2016, 22(4):1024-1030.
[7]
WANG Z, LUO W, CHENG S, et al. Ralstonia solanacearum-a soil borne hidden enemy of plants:research development in management strategies, their action mechanism and challenges[J]. Frontiers in plant science, 2023, 14:1141902.
[8]
JAMES C R. Making greater use of introduced microorganisms for biological control of plant pathogens[J]. Annual review of phytopathology, 1993, 31:53-80.
[9]
袁英英, 李敏清, 胡伟, 等. 生物有机肥对番茄青枯病的防效及对土壤微生物的影响[J]. 农业环境科学学报, 2011, 30(7):1344-1350.
[10]
曹明慧, 冉炜, 杨兴明, 等. 烟草黑胫病拮抗菌的筛选及其生物效应[J]. 土壤学报, 2011, 48(1):151-159.
[11]
SHAH A, NAZARI M, ANTAR M, et al. PGPR in agriculture: A sustainable approach to increasing climate change resilience[J]. Frontiers in sustainable food systems, 2021, 5:211-232.
[12]
PRABHUKARTHIKEYAN S R, KEERTHANA U, RAGUCHANDER T. Antibiotic-producing Pseudomonas fluorescens mediates rhizome rot disease resistance and promotes plant growth in turmeric plants[J]. Microbiological research, 2018, 210:65-73.
Rhizome rot of turmeric caused by Pythium aphanidermatum is a major threat to turmeric-cultivating areas of India. This study intends to evaluate the performance of fluorescent pseudomonads against Rhizome rot disease and understand the resistance mechanism in Turmeric plants. Fluorescent pseudomonads were screened against Pythium aphanidermatum using dual culture. Selected strains were evaluated for the performance of growth promoting attributes and the presence of antibiotic genes through PCR analysis. Strain FP7 recorded the maximum percent inhibition of P. aphanidermatum under in vitro conditions. Strains FP7 and TPF54 both increased plant growth in turmeric plants in vitro. Strain FP7 alone contained all the evaluated antibiotic biosynthetic genes. Talc and liquid-based formulations were prepared with effective strain and tested for its biocontrol activities under both glasshouse and field conditions. Enzymatic activities of the induced defense enzymes such as PO, PPO, PAL, CAT and SOD were estimated and subjected to spectrophotometric analysis. A combination of rhizome dip and soil drench of FP7 liquid formulation treatment remarkably recorded the minimum disease incidence, higher defense enzymes, maximum plant growth and yield under glasshouse and field conditions. Application of strain FP7 increased the defense molecules, plant growth and yield in turmeric plants thereby reducing the incidence of rhizome rot disease. Moreover, this study has a potential to be adopted for sustainable and eco-friendly turmeric production.Copyright © 2018 Elsevier GmbH. All rights reserved.
[13]
SAH S, KRISHNANI S, SINGH R. Pseudomonas mediated nutritional and growth promotional activities for sustainable food security[J]. Current research in microbial sciences, 2021, 2:100084.
[14]
赵娜, 蔡昆争, 汪国平, 等. 家畜堆肥诱导番茄对青枯病的抗性及其生理机制[J]. 农业环境科学学报, 2008, 27(5):2058-2063.
[15]
鲍士旦. 土壤农化分析(第三版)[M]. 北京: 中国农业出版社, 1981:14-20.
[16]
VESTBERG M, KUKKONEN S, SAARI K, et al. Microbial inoculation for improving the growth and health of micropropagated strawberry[J]. Applied soil ecology, 2004, 27(3):243-258.
[17]
曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007:28-29,57-59,68-70.
[18]
高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006:200-201.
[19]
关松荫. 土壤酶及其研究法[M]. 北京: 中国农业出版社, 1986:274-324.
[20]
沈萍, 陈向东. 微生物学实验(5版)[M]. 北京: 高等教育出版, 2018:56,89.
[21]
SINGH R P, TIWARI S, SINGH M, et al. Important diseases of greenhouse crops and their integrated management: A review[J]. Journal of entomology and zoology studies, 2020, 8(1):962-970.
[22]
黄剑, 王健, 李晓阳, 等. 氨基丁酸与有机肥料配施对连作马铃薯产量、品质和土壤活性的影响[J]. 天津农业科学, 2025, 31(7):79-84.
[23]
刘晓. 微生物菌肥在农业生产中的应用研究[J]. 河南农业, 2021,(17):14-15.
[24]
TANG T T, SUN X, LIU Q, et al. Treatment with organic manure inoculated with a biocontrol agent induces soil bacterial communities to inhibit tomato fusarium wilt disease[J]. Frontiers in microbiology, 2023, 13:1006878.
Organic manure, plant growth-promoting microorganisms, and biocontrol agents are widely used to sustainably control soil-borne diseases. However, how and whether organic manure inoculated with biocontrol agents alters soil microbiota and reduces disease severity is poorly understood.
[25]
李桂花, 谭琳, 沈程文, 等. 茶树根腐病拮抗放线菌分离、鉴定及生防潜能研究[J]. 植物保护, 2024, 50(5):87-96,204.
[26]
WANG B, YANG B, PENG H, et al. Genome sequence and comparative analysis of fungal antagonistic strain Bacillus velezensis LJBV19[J]. Folia microbiologica, 2023, 68(1):73-86.
[27]
WU H S, YANG X N, FAN J Q, et al. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms[J]. BioControl, 2009, 54:287-300.
[28]
蔡燕飞, 廖宗文, 章家恩, 等. 生态有机肥对番茄青枯病及土壤微生物多样性的影响[J]. 应用生态学报, 2003, 14(3):349-353.
采用田间小区试验和FAMEs分析研究了不同用量生态有机肥对番茄青枯病的抑制效果和土壤微生物的影响。结果表明,连作地番茄青枯病发生率为100%,施生态有机肥显著地降低了连作地番茄青枯病的发生。用量为1kg&middot;m^-2和0.5kg&middot;m^-2时,番茄青枯病发生率分别为39%和50%,两种施肥量间差异不显著。施生态有机肥后土壤微生物群落脂肪酸指纹发生明显改变,革兰氏阴性菌生物标记物cyc17:0的含量增加,真菌生物标记物c18:2w6的含量增加,作为AM菌根的生物标记物c16:111c呈上升趋势。聚类分析表明,对照和施生态有机肥处理土壤微生物脂肪酸指纹之间的最短距离是1.08,不同施用量处理之间的最短距离是0.70。施用生态有机肥能调控土壤微生物群落结构,促进有益微生物的生长,增强土壤生态系统的稳定性和抑病性,从而提高了土壤质量。
[29]
杨囡君, 刘新社, 决超. 不同土壤改良措施对连作黄瓜幼苗生理特性和产量及品质的影响[J]. 江苏农业科学, 2024, 52(8):144-150.
[30]
薛磊, 凡莉莉, 李云鸽, 等. 生物菌肥不同施用量对鼓节竹发笋期生长和光合特性的影响[J]. 热带作物学报, 2018, 39(7):1332-1337.
[31]
郑国保, 孔德杰, 张源沛, 等. 不同灌溉定额对设施茄子光合特性和产量的影响[J]. 节水灌溉, 2010(12):28-30,34.
为研究不同灌水定额对设施茄子叶片光合特性及产量的影响,在宁夏盐池进行小区栽培试验。结果表明:灌溉定额675、900mm的处理叶片净光合速率影响显著低于灌溉113mm的处理。随着灌水量的增加,叶片蒸腾速率、气孔导度、细胞间CO2浓度逐渐增加,Fo有着先减少后增加的趋势,Fm、Fv随着灌水定额的增加有先增加后减少的趋势。反映了水分亏缺和水分过量都会影响光化学效率、潜在活性、捕获激发能的效率、光和电子的传递。水分利用效率随着灌水量的增加而逐渐的减少,根据不同灌水处理下茄子的生理性状、经济效益和土壤水分的可持续利用原则,最佳的灌溉量为550mm~700mm。
[32]
任金凤. 接种荧光假单胞菌对花生根腐病的防治效应研究[D]. 徐州: 江苏师范大学, 2024.
PDF(1833 KB)

Accesses

Citation

Detail

Sections
Recommended

/