Efficient nitrogen fertilization is critical for maximizing crop productivity while minimizing environmental and health risks. Red beet baby leaves are valued for their vibrant color, flavor, and antioxidant content, particularly betalains, but they are also prone to accumulating antinutritional compounds such as nitrate and oxalate. Excessive nitrogen supply can exacerbate this accumulation, highlighting the need to optimize nitrate input to balance yield, nutritional quality, and safety. This study examined how different nitrate concentrations (1 mM and 10 mM NO3−) in hydroponic systems influence red beet baby leaf yield, quality, and levels of beneficial and harmful compounds. The plants were sampled at 10 and 17 days after planting (DAP), and the effects of the treatments in relation to plant age were assessed. Both sampling time and nitrate concentration significantly influenced red beet baby leaf growth and quality. Extending cultivation to 17 days improved yield and antioxidant levels (phenols, flavonoids, betalains) but also increased soluble oxalates. Low nitrate (1 mM) reduced both yield and antioxidant content, regardless of harvest time. However, after 17 days, low nitrate also lowered total oxalate levels, likely due to increased oxalate oxidase activity. Although 1 mM nitrate reduces fertilizer input, it compromises yield and quality. Therefore, intermediate nitrate levels should be explored to optimize both fertilizer use and product quality.

The influence of high hydrostatic pressure (HHP) and supercritical carbon dioxide (SCCD) on the bioaccessibility of betalains in beetroot (Beta vulgaris L.) juice was investigated. Freshly squeezed juice (FJ) was treated at a mild temperature of 45 °C for 10 min (T45), pasteurization at 85 °C for 10 min (T85), HHP at 200, 400, and 500 MPa at 20 °C for 5 min (HHP200, HHP400, HHP500) and SCCD at 10, 30 and 60 MPa at 45 °C for 10 min (SCCD10, SCCD30, SCCD60). The juice was subjected to an in vitro digestion system equipped with dialysis. The content of betalains was measured with the aid of a High-Performance Liquid Chromatography (HPLC), the antioxidant capacity (AC) (ABTS•+, DPPH•) was analyzed during each digestion step, and the bioaccessibility of betacyanins and betaxanthins was assessed. The SCCD at 30 and 60 MPa significantly increased pigments’ bioaccessibility compared with other samples. The 30 MPa proved particularly advantageous, as it increased the bioaccessibility of the total betacyanins and the betaxanthins by 58% and 64%, respectively, compared to the T85 samples. Additionally, higher bioaccessibility of betacyanins was noted in HHP200 and HHP400, by 35% and 32%, respectively, compared to FJ, T45, and T85 samples. AC measured by ABTS•+ and DPPH• assays were not unequivocal. However, both assays showed significantly higher AC in SCCD60 compared to T85 (21% and 31%, respectively). This research contributed to the extended use of the HHP and/or SCCD to design food with higher health-promoting potentials.

The contemporary demands for massive reductions in industrial pollution caused by the transport sector, especially in large urban agglomerations, compel local and national authorities to propose, develop, and implement programs and policies that have the ultimate goal of significantly reducing (or eliminating) pollution. The aim of this article is to provide a primary analysis of the effectiveness of Romanian government policies in terms of reducing pollution (CO2 emissions) caused by transportation (due to the “Rabla Plus” (RP) program, through which financial subsidies are granted for the purchase of a new plug-in hybrid electric vehicles (PHEVs) or battery electric vehicle (BEVs)). After analyzing the justification for the use of low-emission and electric vehicles in traffic (as a major solution to eliminate pollution), a comparative analysis of energy-efficient transport for Romania and Europe is presented in order to identify the directions in which it is necessary to develop and implement government policies specifically in Romania, considering a series of indicators chosen and considered by the authors to be important, including CO2 emissions compared with the size of the road infrastructure, the number of registered vehicles, the number of passengers transported, and the quantity of goods transported. With the identification of the ability of government programs to encourage the acquisition and use of low-emission and electric vehicles in traffic, the efficiency achieved is calculated in terms of the net CO2 emissions eliminated (average values of 1949.23 CO2 tons/year and 1.71 CO2 tons/vehicle). Furthermore, this aspect is also beneficial for analyses in terms of the economic costs involved (the associated costs are estimated to be 7034.17 EUR/ton of CO2 eliminated from the transportation sector), identifying new directions of action that are more cost-effective and sustainable and on which government policies should focus in the future.