This study optimized key cultivation parameters of a wild Sanghuangporus sp., aiming to provide a theoretical basis for efficient cultivation of Sanghuangporus sp.. Using a wild Sanghuangporus sp. as material, PDA medium was employed for mycelial isolation and purification culture, and the effects of different temperatures and nutritional conditions on the mycelial growth of this Sanghuangporus sp. were investigated. The results showed that 30℃ was more favorable than 20℃ for the germination and growth of Sanghuangporus sp. mycelia. In the carbon source experiment, the starch treatment group exhibited the fastest mycelial growth rate (3.5 mm/d), with significant differences compared to other groups, followed by the glucose treatment group. In the nitrogen source experiment, the beef extract treatment group had the shortest mycelial germination time, with robust and rapidly growing mycelia; the glycine treatment group showed prolonged germination time and slow growth. In the inorganic salt experiment, calcium chloride had the best promoting effect on Sanghuangporus sp. mycelia, with a daily mycelial growth of 3.5 mm/d, followed by the sodium chloride treatment group. The mycelial growth of this Sanghuangporus sp. strain is highly sensitive to temperature and nitrogen source, and appropriate culture temperature and nitrogen source type are key factors for promoting efficient mycelial growth.