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Effects of Bacillus flexus E1 Combined with Organic Fertilizer Application on Leaching Desalination and Fertility Enhancement of Saline-alkali Soil
LIUBinghua, LIUXinghong, PENGLin, SUNMingjie, WANGBo, LIUFangchun, MAHailin
Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (19) : 123-129.
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Abbreviation (ISO4): Chin Agric Sci Bull
Editor in chief: Yulong YIN
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Effects of Bacillus flexus E1 Combined with Organic Fertilizer Application on Leaching Desalination and Fertility Enhancement of Saline-alkali Soil
To investigate the effects of combined application of bacterial agent and organic fertilizer on the remediation of coastal saline-alkali soil, a simulated indoor soil column leaching experiment was conducted. Different treatments, including BF (bacterial agent B. flexus E1 alone), OC (organic fertilizer alone), BO (combined application of B. flexus E1 and organic fertilizer), and CK (control), were compared for their effects on leaching rate, desalination rate, water-stable aggregates (>0.25 mm) and soil nutrients. The results showed that the amendments significantly influenced the above indicators. Compared with CK, the leaching rates of the BF, OC, and BO treatments increased by 1.4, 1.3, and 1.0 times, respectively, while the desalination rates were significantly increased by 223.6%, 237.3%, and 151.0%, respectively. BO demonstrated the best improvement in soil structure and nutrient enhancement, with water-stable aggregates, organic matter, alkali-hydrolyzable nitrogen, available phosphorus, and available potassium increasing by 104.6%, 128.3%, 594.9%, 212.0% and 233.1%, respectively, compared to CK. The leaching rate and desalination rate were significantly positively correlated with the aggregate content. The ranking of improvement effectiveness across treatments was BO>BF>OC>CK. The combined application of B. flexus E1 and organic fertilizer could effectively improve soil structure, enhance nutrient levels, and promote salt leaching through synergistic effects, representing an efficient strategy for the remediation of coastal saline-alkali soil.
saline-alkali soil / Bacillus flexus / organic fertilizer / desalination rate / water-stable macroaggregates / principal component analysis
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设施土壤次生盐渍化已成为制约设施农业发展的主要问题,为验证施用有机物料对次生盐渍化设施土壤的修复效果,本研究采用田间小区试验,以河北永清典型次生盐渍化设施土壤为研究对象,选择牛粪和生物炭两种有机物料并结合微生物菌剂作为外源有机物料,处理包括不施用有机物料(CK)、单施牛粪(DM)、单施生物炭(BC)、牛粪配施菌剂(DM+MI)、生物炭配施菌剂(BC+MI),通过分析各处理土壤的水溶性盐分总量、各盐分离子以及植株生长状况,探讨了有机物料对设施土壤次生盐渍化的改良效果。结果表明:施用有机物料和微生物菌剂可以不同程度的减轻土壤次生盐渍化。与对照相比,对降低土壤盐分效果最显著的为DM+MI处理,最高可降低土壤可溶盐总量18.26%,以及Na<sup>+</sup>、Ca<sup>2+</sup>与Mg<sup>2+</sup>含量24.1%、18.75%、24.29%,其他降盐效果表现为DM处理>BC+MI处理>BC处理。施用有机物料对辣椒植株的生长有一定的促进作用,至第10周时显著增加株高与茎粗达10.40%~16.00%和19.50%~21.30%。对辣椒植株生长与土壤水溶性盐分含量的相关性分析表明,在设施次生盐渍化土壤中,辣椒植株生长与土壤中Ca<sup>2+</sup>和Mg<sup>2+</sup>的含量呈显著负相关关系。综上,有机物料配施微生物菌剂能改善土壤条件,降低土壤含盐量,缓解设施土壤次生盐渍化状况,并促进辣椒正常生长。
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为明确微生物菌剂在内蒙古河套地区中度盐碱土壤改良中的作用,可为盐碱土壤质量提升提供技术支撑。采用大田随机区组试验设计方法,以空白处理(CK)为对照,设置3种微生物菌剂处理,包括丹路牌微生物菌剂(DL)、自主研发的复合微生物菌剂BZ1<sup>T</sup>/1-15(由芽孢杆菌、芽单胞菌和草炭组成),其使用量分别为570 kg·hm<sup>-2</sup>(BL1)和1140 kg·hm<sup>-2</sup>(BL2)。分析不同处理措施下的土壤盐分、养分以及细菌群落结构组成和多样性的变化特征。结果表明:施用微生物菌剂(DL、BL)均可显著降低土壤盐分和pH值,其中BL2处理对降低土壤盐分的效果最显著,与CK、DL、BL1相比分别降低了14.0%、4.2%、7.4%(P<0.05)。同时,施用BL1和BL2微生物菌剂对提高根际土壤的碱解氮、速效钾和有效磷含量有显著作用,其中BL1、BL2处理的碱解氮含量较CK和DL分别显著提高26.4%和11.67%、50.94%和33.33%,BL2处理的速效钾含量较CK、DL和BL1分别显著提高24.1%、24.7%和11.1%(P<0.05)。高通量测序结果表明,BL2处理的丰富度指数(ACE、Chao1)显著高于其他处理,还可显著提升放线菌门、厚壁菌门和蓝细菌门的优势菌群丰度,另外BL1和BL2处理显著降低了酸杆菌门、芽单胞菌门、拟杆菌门、硝化螺旋菌门和浮霉菌门细菌菌群的相对丰度(P<0.05)。相关性分析结果显示,放线菌门与土壤pH值、碱解氮分别呈显著负相关、正相关(r分别为-0.581*、0.595*);土壤速效钾与变形菌门、酸杆菌门和绿弯菌门分别呈极显著负相关、正相关和正相关(r分别为-0.753**、0.503*和0.569*)。因子分析结果表明,有机质、盐分和养分等是影响土壤细菌群落结构的主控环境因子,因为其总共解释了67.0%的群落变化;它们的贡献率依次为:土壤有机质>盐分>速效钾>pH>有效磷>碱解氮。另外,BL1和BL2菌剂的施用对提高向日葵的产量有显著作用,且作物产量与养分含量呈极显著正相关关系。因此,施用微生物菌剂不仅可以降低土壤盐分和pH值,而且还可显著提高土壤养分含量和作物产量,改善细菌菌群结构。该研究可以为微生物菌剂在内蒙古河套灌区盐碱土壤改良方面提供试验基础和参考。
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张恩, 王彬, 白小龙, 等. 不同改良物料对内蒙河套平原盐碱土壤改良效果研究[J]. 中国农学通报, 2024, 40(32):44-49.
为探究不同改良物料对盐碱土壤理化性质及玉米产量的影响,在内蒙古土默特右旗中度盐碱地上开展了脱硫石膏、新型改良剂和有机肥对盐碱土壤改良和玉米生长的影响研究。结果表明:(1)施用改良物料降低了0~20 cm和20~40 cm土层土壤pH和全盐含量,其中,T<sub>4</sub>处理对土壤pH降低效果最好,不同土层土壤pH较对照分别显著下降了8.38%和6.65%;T<sub>6</sub>处理对0~20 cm土层盐分抑制效果最好,较对照显著下降了44.84%,T<sub>4</sub>处理对20~40 cm土层盐分抑制效果最好,较对照显著下降了44.84%。(2)施用改良物料增加了0~20 cm和20~40 cm土层土壤速效磷、速效钾和碱解氮含量,平均增幅分别为15.8%~65.48%、3.06%~19.07%和3.45%~20.24%。(3)施用改良物料增加了不同生育时期玉米株高、茎粗,提高了玉米产量和产量构成,T<sub>7</sub>处理较其他改良处理对玉米生长和产量提升效果较好,玉米株高、茎粗、百粒重、产量较对照分别显著提高了26.21%、20.64%、21.56%、55.21%。综上,施用改良物料可以改善盐碱土壤质量,提升土壤养分,促进玉米生长和提高玉米产量。
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武晋民, 白小龙, 张恩, 等. 改良物料投入对内蒙盐碱地土壤理化性状、玉米光合特性及产量的影响[J]. 西北农业学报, 2025, 34(8):1467-1475.
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张贵芹, 王洪章, 郭新送, 等. 有机物料投入对滨海盐渍地土壤理化性状和夏玉米产量形成的影响[J]. 作物学报, 2024, 50:2323-2334.
探究不同有机物料投入对滨海盐碱地土壤理化性质及玉米生长发育的调控作用, 为滨海盐碱地夏玉米生产提供理论依据。试验于2021—2022年夏玉米生长季在山东省滨州市无棣县滨海盐碱型农田进行。有机物料类型及用量分别为腐植酸(3000 kg hm<sup>-2</sup>, HA)、生物炭(15,000 kg hm<sup>-2</sup>, BC)、生物有机肥(15,000 kg hm<sup>-2</sup>, BO), 以不添加有机物料为对照(CK), 探究0~40 cm土层土壤容重、总孔隙度、田间持水量、pH值、电导率及总有机碳含量的变化, 分析不同有机物料投入对玉米地上部干物质积累和产量形成的影响。结果表明, 腐植酸、生物炭和生物有机肥的施用均可在一定程度上改善盐碱土理化性质。3种有机物料连续处理能有效降低表层土壤容重, 增加土壤总孔隙度和田间持水量; 可显著降低0~10 cm土层pH, 分别平均降低0.17、0.08和0.20。连续施用2年后, 腐植酸显著降低0~40 cm土层电导率, 平均降低32.74%; 3种有机物料显著增加0~20 cm土层中总有机碳的含量, 其中生物炭处理能显著增加57.99%。腐植酸和生物有机肥处理显著增加夏玉米地上部干物质积累量和产量; 连续使用2年后腐植酸处理产量显著增加了11.01%, 表现较好。综上所述, 本试验条件下腐植酸施用后能改善土壤物理结构、降低0~10 cm土层pH、提高土壤中有机碳含量、显著降低土壤电导率, 促进夏玉米地上部干物质积累、提高籽粒产量, 且连续施用2年后较CK增加纯收益。故而腐植酸可作为改良滨海盐碱地土壤理化性状、促进夏玉米生长发育的有机物料。生物有机肥的施用可改善土壤理化性状, 但仍需进行定位试验以验证其长期经济效应。
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蔡婧晖, 黄立华, 刘伯顺, 等. 盐碱土与非盐碱土团聚体差异、改良影响及启示[J]. 水土保持学报, 2025, 39(5):1-12.
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马馥扬, 曹巍巍, 彭昊, 等. 不同改良剂对盐渍土壤的综合改良效应研究[J]. 灌溉排水学报, 2025, 44(1):89-96.
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董心亮, 王金涛, 田柳, 等. 盐渍化土壤团聚体和微生物与有机质关系研究进展[J]. 中国生态农业学报, 2023, 31(3):364-372.
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殷启杰, 姜建武, 殷汉琴, 等. 有机物料投入对新垦耕地土壤养分和微生物代谢的影响[J]. 应用生态学报, 2025, 36(4):969-983.
新垦耕地固碳培肥过程中,土壤微生物代谢对有机物料投入的响应及主控因素尚不明确。本研究于2022年在浙江建德进行大田定位试验,以常规化肥(NPK)为对照,等碳原则下设置NPK基础上增施秸秆+有机肥(MS)、有机肥(M)、秸秆炭基有机肥(MBF)、秸秆源生物质炭(MB)处理,探究不同有机物料投入对稻麦轮作下新垦耕地土壤养分、有机碳组分、胞外酶活性、微生物代谢和碳利用效率的影响。结果表明: 与NPK相比,有机物料投入均提高了土壤总氮磷钾养分、有机碳含量和微生物活性。土壤养分表现为M>MBF>MS>MB,作物产量表现为MS>M>MBF>MB。活性有机碳含量表现为M>MS>MBF>MB,其中M处理最高,较对照提高了91.7%;惰性有机碳含量表现为MB>MBF>M>MS,MB处理最高,较对照提高了160.7%。微生物生物量表现为M>MS>MBF>MB,M处理微生物生物量碳、氮、磷平均分别较对照提高81.1%、140.9%、261.1%。胞外酶活性表现为MS>M>MB>MBF,MS处理碳循环相关酶(β-葡萄糖苷酶、β-木糖苷酶、纤维素酶)活性平均分别较对照提高176.3%、180.4%、439.2%,氮循环相关酶(N-乙酰-β-葡萄糖苷酶)活性较对照提高331.4%。Mantel相关性分析和冗余分析表明,溶解性有机碳和微生物生物量碳分别是小麦和水稻种植季土壤胞外酶活性的最强驱动因子。酶向量模型和偏最小二乘路径模型分析显示,新垦耕地土壤微生物代谢同时受到碳和磷限制,有机物料投入通过提高新垦耕地土壤速效养分直接缓解磷限制,通过增加土壤活性有机碳含量降低了微生物碳利用效率。综上,有机物料投入在新垦耕地固碳培肥中具有积极作用,其中秸秆炭基有机肥处理固碳培肥综合作用最好。
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The coastal saline-alkali soil in eastern China is an important reserve arable land resource. Adding exogenous organic material is an effective way to improve soil fertility and promote the conversion of saline-alkali soil to agricultural soil. In this study, a field plot experiment was used to investigate the influences of different organic materials (vinegar residue, VR; sewage sludge, SS; vermicompost, VC) on the reduction in salinity-alkalinity barrier factors, the accumulation of soil organic carbon (SOC), and the improvement in soil fertility in saline-alkali soil. The results indicated that applying different types of exogenous organic materials reduced soil electrical conductivity (EC) and pH, promoted SOC accumulation, and increased the barley yield. With the same application rate, VR application was more beneficial in reducing soil EC and pH, accumulating SOC, and increasing barley yield compared to SS and VC applications. In particular, the barley yield with VR application was higher than that with SS and VC applications by 18.4% and 26.6% on average, respectively, during the two-year experiment. Correlation and path analysis revealed that the barley yield was significantly negatively correlated with soil barrier factors (EC and pH), but EC in SS and VC-treated soils had an indirect negative effect on barley yield, while EC in VR-treated soil had a direct negative effect (−2.24). In addition, the direct (−4.46) and indirect (5.39) contributions of SOC to barley yield were higher with VR than those with SS and VC, while the direct contribution of soil aggregate to barley yield was lower with VR than that with SS and VC. Therefore, compared with SS and VC applications, VR application led to a fast reduction in soil barrier factors and the rapid accumulation of SOC, which were more beneficial for increasing barley yields in saline-alkali soil.
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刘国辉, 买文选, 田长彦. 施用有机肥对盐碱土的改良效果:Meta分析[J]. 农业资源与环境学报, 2023, 40(1):86-96.
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秦学, 崔钰爽, 黄魁, 等. 有机肥调控盐碱土氮循环的微生物驱动机制[J]. 应用生态学报, 2025, 36(9):2694-2702.
探明有机肥施用对盐碱土氮循环的调控机制,解析微生物群落-功能基因协同作用路径,可为盐碱土可持续改良提供理论依据。本研究基于中国科学院南皮生态农业试验站4年小麦定位试验(2018—2022年),设置化肥(复合肥N∶P<sub>2</sub>O<sub>5</sub>∶K<sub>2</sub>O为18∶22∶5)和有机肥(等氮量施用腐熟牛粪有机肥)2个处理,通过常规养分分析和高通量宏基因组测序技术,结合Mantel检验与功能基因关联分析法,分析有机肥对盐碱土氮转化微生物群落的影响及其调控机制。结果表明: 与化肥处理相比,施用有机肥处理耕层土壤总氮和碱解氮含量分别显著提高了26.9%和34.5%,硝态氮含量显著降低了28.3%。施用有机肥显著提高了微生物ACE指数,但Shannon指数无显著变化。有机肥处理提高了假双头斧形菌属(耐盐菌属)的相对丰度,增幅达517.0%,显著改变了微生物群落结构,显著上调了硝酸盐还原基因(napA)和反硝化基因(norB/nosZ)丰度,协同提高了谷氨酰胺合成酶的相对丰度。Mantel检验揭示,假双头斧形菌属相对丰度与土壤总氮、碱解氮和铵态氮含量均呈显著正相关,norB基因相对丰度与上述3个氮素指标亦呈显著正相关。表明有机肥通过调控关键菌群和功能基因丰度改变了盐碱土氮代谢网络,缓解了盐碱土的氮素阻滞。
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