Effects of Combined Application of Nitrogen, Phosphorus and Potassium on Soil Properties and Tobacco Yield and Quality Under Alfalfa-Tobacco Rotation System

XUXingyang, YANGZongyun, GENGYu, MAZuoyu, WANGXiaoyun, HOUYongqiang, PANSiguang, PANYihong

Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (19) : 51-59.

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Chin Agric Sci Bull ›› 2026, Vol. 42 ›› Issue (19) : 51-59. DOI: 10.11924/j.issn.1000-6850.casb2026-0348

Effects of Combined Application of Nitrogen, Phosphorus and Potassium on Soil Properties and Tobacco Yield and Quality Under Alfalfa-Tobacco Rotation System

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Abstract

To explore the effect of a ‘two-year alfalfa-one-year tobacco’ rotation system and the appropriate combined application scheme of nitrogen, phosphorus and potassium fertilizers, and to alleviate the continuous tobacco cultivation obstacles, ‘K326’ tobacco and the alfalfa variety ‘Algonquin’ were used as materials for the experiment in the tobacco-growing area of Xundian, Yunnan Province. A L9(33) orthogonal design was adopted to set up 9 combined application treatments of nitrogen, phosphorus and potassium fertilizers and 1 blank control (CK). The effects of different fertilization strategies on soil physical and chemical properties and tobacco yield and quality under this planting system were analyzed. The results showed that the fresh weight and dry weight of alfalfa in the second year were 31.6% and 44.2% higher than those in the first year, respectively, and the best cutting period was in the late spring and early summer. The combined application of fertilizers had no significant effect on soil pH and water-soluble chlorine content (P>0.05). The T7 treatment (N 127.5 kg/hm2, P2O5 82.5 kg/hm2, K2O 382.5 kg/hm2) had the best effect on improving soil organic matter and other nutrient indicators. The agronomic traits were the best in the T7 treatment, and the growth vigor of tobacco plants was the best. In terms of economic traits, the T7 treatment had the highest yield, and the T6 treatment (N 105.0 kg/hm2, P2O5 112.5 kg/hm2, K2O 247.5 kg/hm2) had the best comprehensive benefits, with a value of 67984.5 yuan/hm2 and a proportion of high-quality tobacco of 75.83%. The T3 (N 82.5 kg/hm2, P2O5 112.5 kg/hm2, K2O 382.5 kg/hm2), T4 (N 105.0 kg/hm2, P2O5 82.5 kg/hm2, K2O 315.0 kg/hm2), T6 and T7 treatments had excellent tobacco leaf appearance quality, and the T4, T6 and T7 treatments had higher chemical component availability indices of middle tobacco leaves. The blank control was significantly lower than the fertilization treatments (T1-T9) in all indicators (P<0.05), and the growth and yield and quality of tobacco were severely inhibited. In conclusion, the ‘two-year alfalfa + one-year tobacco’ rotation system can improve the basic fertility of tobacco field soil. Combined with the appropriate application of nitrogen, phosphorus and potassium fertilizers, it can significantly improve the soil and enhance the yield and quality of tobacco. Among them, the T6 treatment is the optimal fertilization treatment that takes into account both yield and quality and economic benefits, and the T7 treatment is the appropriate fertilization treatment that focuses on increasing yield.

Key words

flue-cured tobacco / alfalfa / rotation / combined application of nitrogen, phosphorus and potassium / soil physical and chemical properties / economic traits

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XU Xingyang , YANG Zongyun , GENG Yu , et al . Effects of Combined Application of Nitrogen, Phosphorus and Potassium on Soil Properties and Tobacco Yield and Quality Under Alfalfa-Tobacco Rotation System[J]. Chinese Agricultural Science Bulletin. 2026, 42(19): 51-59 https://doi.org/10.11924/j.issn.1000-6850.casb2026-0348

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The long-term physicochemical responses of aeolian sandy soil aggregates to different crop rotation patterns are poorly understood. Here, we collected soil samples from the 0 to 20 cm tillage layer of continuous maize crop and alfalfa–maize rotation plots situated on the edge of the Zhangye Oasis, Northwest China. These samples were analyzed to quantify the influence of both cropping patterns on the structure, carbon content, and nitrogen content of aeolian sandy soils. When compared with long-term continuous maize cropping, planting alfalfa–maize rotation system significantly increased the mass fraction of macro-aggregates with sizes of > 2 mm and 0.25–2 mm from 8.7 to 12.1% and 19.1 to 21.2%, respectively, but decreased the mass fraction of micro-aggregates (0.053–0.25 mm) from 8.1 to 6.2%. Further, there was no significant difference in the content of silt and clay particles between each system. The alfalfa–maize rotation increased the stability of aggregates from 32 to 37%, representing an increase of 15.6%. Soil organic carbon, inorganic carbon, and total nitrogen were mainly enriched in macro-aggregates with sizes of > 2 mm, and silt and clay fractions for both cropping patterns. Implementation of a rotation pattern increased organic carbon contents by 27.2%, 25.6%, 26.7%, and 27.6%, inorganic carbon contents by 14.4%, 4.5%, 53.3%, and 21.0%, and total nitrogen contents by 29.7%, 7.0%, 4.2%, and 50.0% in aggregate particle sizes of > 2 mm, 0.25–2 mm, 0.053–0.25 mm, and < 0.053 mm, respectively, when compared to continuous maize cropping. The alfalfa–maize crop rotation can therefore effectively improve soil aggregate composition and aggregate stability, alongside organic carbon content, inorganic carbon content, total nitrogen content, and their storage capacity. This system thus represents a soil cultivation technique that can increase the soil carbon sequestration capacity in the oasis zone of Northwest China.
[25]
ZHANG Y, LI T, WU H, et al. Effect of different fertilization practices on soil microbial community in a wheat-maize rotation system[J]. Sustainability, 2019, 11(15):4088.
Little information is known about the effects of different fertilization practices on soil microbiome in intensively managed crop rotations. The objective of this research was to investigate the response of microbial community composition (phospholipid fatty acid, PLFA) and extracellular enzyme activity to fertilization treatments through a three-year experiment. Treatments were: Control (without fertilizer, CK), chemical fertilizer (NPK), NPK + pig manure (NPKM), NPK + straw (NPKS), and NPK + both manure and straw (NPKMS). We found that fertilization had no effect on the microbial abundance except arbuscular mycorrhizal fungi (AMF) PLFA. Soil microbial community composition was significantly affected by crop species and to a lesser extent by fertilization, with a greater influence on the wheat harvest. In addition, soil enzyme activities were enhanced by fertilization, especially in wheat season. Over three years, compared with NPK treatment, addition of organic manure or straw (NPKS and NPKMS) significantly increased the activities of the enzymes except invertase and urease, and the effect was greater at wheat harvest than the maize harvest. Our results indicate that the response of soil microbial community structure and enzyme activities to fertilization takes precedence than microbial biomass in the short term. The temporal variation in soil microbial community structure and enzyme activities in the crop rotation indicate that crop species may be carefully considered for sustainable agricultural intensification management.
[26]
冯浩亮, 韩晓增, 陆欣春, 等. 有机培肥影响土壤团聚体形成与稳定的研究进展[J]. 土壤与作物, 2023, 12(4):393-400.
[27]
余洁, 苗淑杰, 乔云发. 不同类型土壤团聚体稳定机制的研究[J]. 中国农学通报, 2022, 38(14):89-95.
土壤团聚体是土壤生态系统中重要的组成部分,影响着土壤的物理、化学和生物学性质,它的稳定性是受粘结剂和分散剂共同作用的结果。本研究目的在于探讨粘结剂和分散剂对土壤团聚体稳定机制的影响。选取全国10种主要地带性土壤类型(黑土、红壤、黄棕壤、暗棕壤、紫色土、栗钙土、黄绵土、潮土、砖红壤、风沙土)为研究对象,采用湿筛法筛分土壤团聚体并测定分析。结果表明:在10种类型土壤团聚体分布中发现砖红壤>0.25 mm大团聚体含量最高,为86.77%;黑土次之,为76.13%;潮土大团聚体含量最少,仅为42.85%。>2 mm和0.5~1 mm含量对团聚体分布影响最大。各个土壤有机质中黑土有机质含量为46.06 g/kg,显著高于其他土壤,砖红壤次之;粒径<0.053 mm时各土壤有机质差异最显著,粒径>2 mm时与全土有机质趋势一致。栗钙土在10种土壤中交换性钠含量最高,为234.48 mg/kg,其次是潮土177.08 mg/kg。从整体看,有机质含量与土壤团聚体稳定性呈正相关(r=0.615),交换性钠与土壤团聚体呈负相关(r=-0.391),有机质与团聚体稳定性的显著相关性在粒径0.053~0.25 mm最明显。土壤团聚体的稳定由有机质与交换性钠共同作用,增加相同单位的有机质与交换性钠,有机质对团聚体的稳定效果是交换性钠分散效果的10倍。为更好的保护土壤,在农业生产中应施用含钠较低的有机肥,减少人类对土壤结构的破坏。
[28]
汤宏, 曾掌权, 李向阳, 等. 水磷耦合烤烟养分吸收分配规律研究[J]. 水土保持学报, 2019, 33(2):294-302.
[29]
李云霞, 杨佳蒴, 李洋洋, 等. 不同移栽期对烟稻轮作烟区烤烟生长发育及产量和质量的影响[J]. 作物杂志, 2025(2):222-227.
[30]
许晓敬, 张小全, 侯冰清, 等. 生态、品种和栽培措施及其互作对烤烟主要化学成分含量的影响[J]. 中国农学通报, 2015, 31(13):62-66.
为探讨生态、品种、栽培措施及其互作对烤烟主要化学成分(还原糖、总氮和烟碱)含量的影响,在河南、山东、陕西3个烟区,以‘豫烟10号’‘、粤烟98’‘、K326’‘、湘烟3号’为研究材料,设置不同的栽培措施,采用方差分析的方法比较烟叶化学成分含量的差异。结果表明:烟叶主要化学成分含量的变异系数在生态间最大,在品种、栽培措施间较小。生态、品种、栽培措施及其互作对还原糖、总氮、烟碱含量均有显著的影响,生态条件对烟叶还原糖、总氮、烟碱含量的贡献率分别为53.92%、24.33%和77.41%,其次是互作效应和品种,栽培处理的贡献率最小。烟叶还原糖和烟碱含量与成熟期和旺长期的降雨量、日照时数关联度较大;烟叶总氮含量与旺长期、成熟期和团棵期的总日照时数关联度最大。生态条件对烟叶化学成分的影响大于品种和栽培措施,是影响烟叶化学成分的主要因素。整个大田期以降雨量和日照时数与烟叶化学成分相关性较大。
[31]
宋鹏, 李慧, 江厚龙, 等. 生物质炭基肥对重庆植烟区烤烟根系发育及产量和品质的影响[J]. 浙江农林大学学报, 2023, 40(6):1232-1240.
[32]
施锘, 朱宏强, 杨梦璇, 等. 不同微生物菌肥对烤烟生长发育、产量及品质的影响[J]. 作物杂志, 2026(1):189-196.
[33]
张嘉雯, 卢绍浩, 赵铭钦, 等. 施氮量对四川雪茄烟叶碳氮代谢及品质的影响[J]. 作物杂志, 2021(4):159-165.
[34]
KHAN A, PENG C, CHEN K, et al. Multi-split nitrogen application via drip irrigation improved dry matter accumulation, nitrogen uptake and utilization efficiency in flue-cured tobacco[J]. BMC plant biology, 2025, 25(1):674.
Flue-cured tobacco (Nicotiana tabacum L.), a key economic crop in China, is highly dependent on nitrogen (N) management. This study evaluated the effects of multi-split N application via drip irrigation on tobacco growth, N accumulation, and nitrogen use efficiency (NUE).A randomized experiment was conducted with 10 treatments (T0-T9) in K326 tobacco grown in nutrient-rich brown soil. The treatments differed in terms of N application timing and proportions.T2 and T3, which applied N twice post-transplant, led to the highest dry weight accumulation in roots, stems, and leaves, with T3 resulting in a 65.8% increase in root dry weight over that of T6. N accumulation followed the order of leaves > stems > roots, with T1 resulting in the highest total N accumulation, a 1.77-fold significant increase compared with that in T0 (p < 0.05). The fertilizer nitrogen (FN) was highest at T1 and T2, reaching nearly 46%, whereas T9 presented the lowest FN, decreasing by 22% compared with that at T1. T1 also retained the most N in the soil, whereas T3 had the highest N residues after the first top dressing. Uneven N applications resulted in significantly lower biomass and NUE.These results highlight the importance of balanced multi-split N applications (T2 and T3) in improving dry matter accumulation, NUE, and crop quality. Optimizing N application through drip irrigation offers a promising approach to improving both yield and N management efficiency in flue-cured tobacco cultivation.© 2025. The Author(s).
[35]
ALLEN J A, REED T D, WILKINSON C A, et al. Impact of nitrogen fertilization rate on cured leaf quality of flue-cured tobacco[R]. Blacksburg: Virginia polytechnic institute and state university, 2023.
[36]
王艳丽, 刘国顺, 丁松爽, 等. 磷用量对烤烟根系及其与地上部关系的影响[J]. 应用生态学报, 2015, 26(5):1440-1446.
以&lsquo;豫烟10号&rsquo;为材料,研究大田生育期内不同磷肥用量对烤烟根干质量等根系指标的影响,以及根系和地上部物质分配、矿质营养积累的差异.结果表明: 施磷能显著促进烤烟根系发育和干物质向地上部的分配,30 kg P<sub>2</sub>O<sub>5</sub>&middot;hm<sup>-2</sup>处理烟株具有最大的根干质量、根体积、根系活力和最小的根冠比.烤烟根系和叶片对养分的最大积累速率依次出现在移栽后57~66 d和44~55 d,施磷不仅能促进烟株对养分的吸收,使养分最大积累速率提早出现,而且能显著促进烟株地上部的养分积累,但当施磷量超过30 kg P<sub>2</sub>O<sub>5</sub>&middot;hm<sup>-2</sup>时,上述促进作用反而减弱.在生产上应合理施磷,以提高烟叶品质.&nbsp;
[37]
王艳丽, 王京, 刘国顺, 等. 磷施用量对烤烟根系生理及叶片光合特性的影响[J]. 植物营养与肥料学报, 2016, 22(2):410-417.
[38]
陈义强, 刘国顺, 习红昂. 基于烟叶品质指数的氮、磷、钾施肥模型[J]. 植物营养与肥料学报, 2013, 19(3):632-643.
[39]
HASANUZZAMAN M, BHUYAN B M H M, NAHAR K, et al. Potassium: A vital regulator of plant responses and tolerance to abiotic stresses[J]. Agronomy, 2018, 8(3):31.
Among the plant nutrients, potassium (K) is one of the vital elements required for plant growth and physiology. Potassium is not only a constituent of the plant structure but it also has a regulatory function in several biochemical processes related to protein synthesis, carbohydrate metabolism, and enzyme activation. Several physiological processes depend on K, such as stomatal regulation and photosynthesis. In recent decades, K was found to provide abiotic stress tolerance. Under salt stress, K helps to maintain ion homeostasis and to regulate the osmotic balance. Under drought stress conditions, K regulates stomatal opening and helps plants adapt to water deficits. Many reports support the notion that K enhances antioxidant defense in plants and therefore protects them from oxidative stress under various environmental adversities. In addition, this element provides some cellular signaling alone or in association with other signaling molecules and phytohormones. Although considerable progress has been made in understanding K-induced abiotic stress tolerance in plants, the exact molecular mechanisms of these protections are still under investigation. In this review, we summarized the recent literature on the biological functions of K, its uptake, its translocation, and its role in plant abiotic stress tolerance.
[40]
HU W, DI Q, WANG Z J, et al. Grafting alleviates potassium stress and improves growth in tobacco[J]. BMC plant biology, 2019, 19(1):130.
Potassium is a nutrient element necessary for tobacco growth. Tobacco leaves with high potassium content are elastic and tough, rich in oil. And the same time, potassium can also improve the scent and aromatic value of flue-cured tobacco by regulating the synthesis of aromatic hydrocarbons in leaves.. It is an important quality indicator for flue-cured tobacco. However, the potassium concentration in tobacco leaves in most areas of China is generally lower than the global standard for high quality tobacco. Two tobacco genotypes were grafted to each other under different potassium levels to test whether potassium content and plant growth can be improved by grafting in tobacco.The growth of tobacco in all treatments was inhibited under potassium starvation, and grafting significantly alleviated this potassium stress in 'Yunyan 87'. The trends in whole plant K uptake and K transfer efficiency to the leaves corresponded to the growth results of the different grafts. The nutrient depletion test results showed that the roots of 'Wufeng No.2' had higher K absorption potential, K affinity, and K inward flow rate. K enrichment circles appeared at the endoderm of the root section in the energy dispersive X-ray figure, indicating that the formation of Casparian strips may be partly responsible for the lower rate of lateral movement of K in the roots of 'Yunyan 87'. Gene expression analysis suggested that energy redistribution at the whole plant level might constitute one strategy for coping with potassium starvation. The feedback regulation effects between scion 'Wufeng No.2' and rootstock 'Yunyan 87' indicated that the transmission of certain signaling substances had occurred during grafting.'Wufeng No.2' tobacco rootstock grafting can increase the K uptake and transport efficiency of 'Yunyan 87' and enhance plant growth under potassium stress. The physiological mechanism of the improved performance of grafted tobacco is related to higher K uptake and utilization ability, improved xylem K loading capacity, and up-regulated expression of genes related to energy supply systems.
[41]
熊莹, 杨俊杰, 蒋佳祝, 等. 山东省不同产区烤烟化学成分差异性及可用性分析[J]. 中国农学通报, 2026, 42(4):210-218.
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