The research on isolation leaves disk culture isolate induced that the system of high efficient plantlet regeneration of was available. Though adding of hormones, the results indicated that hormone combination with 6-BA2.5 mg/L, NAA0.5 mg/L frequency inducting of callus was 86.6%, and the regeneration rate was 83.3%. It is notability affect to the regeneration of the explants when the leaf disk surfaces were kept contacted with culture medium. Add of the solution of AgNO3（4.0mg/L）to the medium at the leaf disk inoculation stage effect directly the shoot regeneration, while markedly increased shoot regeneration frequency at the time after callus formation. The regenerated shoots rooted easily in vitro and the resulted plants grew normally after transplanting in pots.
Chen Yuliang, Zhi Xiaogang, ZhangYanping, Zhang Zhengying
. Isolation Leaf Disks Culture and High Efficient Plantlet Regeneration of Kalanchoe Blossfeldianna[J]. Chinese Agricultural Science Bulletin, 2004
, 20(5)
: 33
-33
.
DOI: 10.11924/j.issn.1000-6850.040533
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