Stem segments and leaves of new shooting, which are from winter shoot treated by FC , was used as inoculating explant. The medium used for producing axillary bud from stem segment is 1/2MS+6-BA0.2~0.5mg/L + NAA 0~0.5mg/L; the medium used for generating adventitious bud is 1/2MS or MS + 6-BA 0.5mg/L + NAA0.5mg/L; medium used for producing rosette buds is MS+6-BA0.3mg/L + NAA0.3mg/L. Regeneration was conducted every 20 days and 20 times of multiplication could be achieved. Rooting medium is 1/2MS or MS. Adding benzimidazole 0.1~0.5mg/L in the medium could promote rooting.
Liu Wenping, Han Yuqin, Nan Xiangri, Zhu Cai
. Study on Rapid Multiplication Techniques by Tissue Culture in Shanxinyang(P.davidiana X P.bollena )[J]. Chinese Agricultural Science Bulletin, 2005
, 21(1)
: 101
-101
.
DOI: 10.11924/j.issn.1000-6850.0501101
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