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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Studies on Extraction of Alkaloids in seeds of Sophora alopecuraides L.

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Online published: 2005-02-04

Abstract

The extraction and separation of Alkaloids in seeds of Sophora alopecuroides were studied in the experiment, the quantitative and qualitative analysis in matrine were also detected. Alkaloids were extracted from seeds by immersing in 0.4% hydrochloric acid, 95% alcohol, 1% ammonia-chloroform and anhydrous alcohol for four times in seven days under the room temperature after crushed, accompanying by delipoid, acidification, alkalization, extraction. Single Alkaloids were separated by TLC method, and quantitative and qualitative analysis was done by GC method. The results suggested that extraction yield of four methods above were 3.67%,2.73%,2.47% and 1.60% respectively. The extracted Alkaloids in each method contained four kinds of single Alkaloids at least by TLC. The extraction Alkaloids of all these four methods contained Kushen Alkaloids detected by GC, and the extraction yield were 8.460%、7.553%、5.675%and0.893% respectively. The results showed that extraction yield by 0.4% hydrochloric acid under room temperature was obviously higher than other solvents, which could be the optimal extraction method of Alkaloids in seeds of Sophora alopecuroides.

Cite this article

Zhang Weimin,Pu Peng, Zhang Degang,Feng Zimao,Zhang Yiyun,Liao Xiaomin . Studies on Extraction of Alkaloids in seeds of Sophora alopecuraides L.[J]. Chinese Agricultural Science Bulletin, 2005 , 21(2) : 7 -7 . DOI: 10.11924/j.issn.1000-6850.050207

References

1秦学功,元英进.苦豆子种子中生物碱的冷浸提取实验研究[J].中草药,2001,32(7):604~606
2江苏新医学院.中药大辞典[M].上海:上海科技出版社,1977:1293~1294
3仲仁山.苦豆子的研究及应用[M].银川:宁夏人发出版社,1980.
4谭焕然,张宝恒.苦参碱抗炎症作用的实验研究[J].中西医结合杂志,1958,5 (2):108~110
5王淑君,陈丰,姚崇舜.苦豆子中生物碱的药物动力学研究[J].中草药,2000,31(5):附1~2
6江西医学院药物研究所病理组.抗肿瘤中草药究Ⅲ?西豆根甲碱的抗癌作用及毒性初步观察[J].江西医学杂志,1980,(10):47
7黄秀梅,李波.苦豆子类生物碱的药理学研究进展[J].中国药事,2002,16(3):175~178
8 黎雪如,戴寿芝,李力,等.苦豆子的七种生物碱对小鼠免疫功能的影响[J].中草药,1987,18(5):22
9赵宝玉.疯草(甘肃棘豆)生物碱系统分析及其毒性的比较病理学研究[D].2001届攻读博士学位研究生学位论文,2001,附录Ⅳ
10秦学功,元英进.高效薄层色谱分离苦豆子生物碱的体系优化[J].中草药,2002,33(6):513~514
11肖崇厚.中药化学[M].上海:上海科学技术出版社,1997.
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