Recently, spermatogonial stem cell (SSC), the postnatal male germ-line stem cells, has been a subject of intense and increasing interest as a result of exciting developments in stem cell research in general and the development of new research tools allowing the isolation, culture and transplantation of these unique cells. SSC is a adult stem cell population resided in the basal compartment of the seminiferous epithelium, which is not only capable of self-renewal and differentiation, but the only adult stem cells in the normal postnatal body that undergo self-renewal throughout life and transmit gene to the progeny. In order to obtain stem cells enough for researches and application, understanding the regulation mechanisms of the proliferation and differentiation of stem cells is the common problem in stem cell research. With the aid of SSCs transplantation, a functional assay for SSCs, the culture conditions of SSCs have been examined deeply in recently years. This review focuses on what is know about the maintenance of SSCs in vitro and discusses the possible problem in future studies.
Zhang Xueming, Li Dexue,, Yue Zhanpeng
. Proliferation and Differentiation of Spermatogonial
Stem Cell (review)[J]. Chinese Agricultural Science Bulletin, 2005
, 21(2)
: 16
-16
.
DOI: 10.11924/j.issn.1000-6850.050216
1Brinster RL. Germline stem cell transplantation and transgenesis. Science, 2002,296(5576): 2174~2175
2Zhang X M, Li D X, Yue Z P, et al. Advances in mammalian spermatogonial stem cell transplantation. Progress in Natural Science, 2004; 14(6):467~471
3Honaramooz A, Behboodi E, Blash S, et al. Germ cell transplantation in goats. Mol Reprod Dev, 2003,64(4):422~428
4Kanatsu-Shinohara M, Ogonuki N, et al. Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells. Hum Reprod, 2003,18(12):2660~2667
5Oatley J M, de Avila D M, Reeves J J, et al. Testis tissue explant culture supports survival and proliferation of bovine spermatogonial stem cells. Biol Reprod, 2004; 70(3):625~631
6McLean D J, Russell L D, Griswold M D. Biological activity and enrichment of spermatogonial stem cells in vitamin A-deficient and hyperthermia-exposed testes from mice based on colonization following germ cell transplantation. Biol Reprod, 2002; 66(5):1374~1379
7Feng L X, Chen Y, Dettin L, et al. Generation and in vitro differentiation of a spermatogonoal cell line. Science, 2002,297:392~395
8Nagano M, Ryu B Y, Brinster C J, et al. Maintenance of mouse male germ line stem cells in vitro. Biol Reprod, 2003;68(6): 2207~2214
9Kubota H, Avarbock M R, Brinster B L. Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells. Biol Reprod, 2004; 71(3):722~731
10 Kanatsu-Shinohara M, Toyokuni S, Shinohara T. CD9 is a surface marker on mouse and rat male germline stem cells. Biol Reprod, 2004,70(1):70~75
11 Izadyar F, den Ouden K, Creemers L B, et al. Proliferation and differentiation of bovine type A spermatogonia during long-term culture. Biol Reprod, 2003, 68(1): 272~281
12 Yin M, Li DX. Effects of SCF, LIF and bFGF on the proliferation of mouse spermatogonial stem cells in vitro. Sheng Wu Gong Cheng Xue Bao, 2002, 18(6): 754~757
13 Miura T, Ohta T, Miura C I, et al. Complementary deoxyribonucleic acid cloning of spermatogonial stem cell renewal factor. Endocrinology, 2003, 144(12):5504~5510
14 Meachem S J, Wreford N G, Stanton P G, et al. Follicle-stimulating hormone is required for the initial phase of spermatogenic restoration in adult rats following gonadotropin suppression. J Androl, 1998.15
15 Dobrinski I, Ogawa T, Avarbock MR, et al. Effect of the GnRH-agonist leuprolide on colonization of recipient testes by donor spermatogonial stem cells after transplantation in mice. Tissue Cell, 2001; 33(2):200~207
16 Jeong D, McLean D J, Griswold M D. Long-term culture and transplantation of murine testicular germ cells. J Androl, 2003, 24(5):661~669
17 Ohta H, Tohda A, Nishimune Y. Proliferation and differentiation of spermatogonial stem cells in the W/Wv mutant mouse testis. Biol Reprod, 2003,69(6): 1815~1821
18 Ohta H, Yomogida K, Dohmae K, et al. Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF. Development, 2000,127(10):2125~2131
19 Piquet-Pellorce C, Dorval-Coiffec I, Pham MD, et al. Leukemia inhibitory factor expression and regulation within the testis. Endocrinology, 2000,141(3):1136~1141
20 Meng X, Lindahl M, Hyvonen M E, et al. Regulation of cell fate decision of undifferentiated spermatogonia by GDNF. Science, 2000,287(5457):1489~1493
21 Kanatsu-Shinohara M, Ogonuki N, Inoue K, et al. Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod, 2003,69(2): 612~616
22 Akama TO, Nakagawa H, Sugihara K, et al. Germ cell survival through carbohydrate-mediated interaction with Sertoli cells. Science, 2002, 295(5552):124~127
23 van Pelt A M M, Roepers-Gajadien H L, Gademan I S, et al. Establishment of cell lines with rat spermatogonial stem cell characteristics. Endocrinology, 2002,143(5):1845~1850
24 Hong Y, Liu T, Zhao H, et al. Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro. Proc Natl Acad Sci USA, 2004,101(21): 8011~8016
