Except one or two period, the quantitiy of oxygen radical and the activities of superoxide dismatase and ascorbic acid peroxidase in the leaves, stems and roots of trans-BSFE gene and its signal peptide and propeptide sequence tobacco were all higher than those of the common tobacco. We infer the change of the quantity of oxygen radical relates with the hydrolyzing activity of BSFE protein, and the change of activities of superoxide dismutase and ascorbic acid hydrogen peroxidase ralate with counter-adaptation to the expression of exogenons BSFE gene and its product accumulation of the transgenic tobacco.
Li Guojing,, Jiang Shuyuan, Wu Zirong, Wang Shuiping, Wang Ruigang,,,
. Influence of Trans-Bacillus subtilis fibrinolytic enzymic gene on Oxygen Radical and Protective Enzyme System in Tobacco[J]. Chinese Agricultural Science Bulletin, 2005
, 21(2)
: 34
-34
.
DOI: 10.11924/j.issn.1000-6850.050234
1王瑞刚,王水平.外源蛋白表达系统及利用植物表达外源蛋白的特点与优势.生物学通报,2003,38(1):11~12
2黄燕,赵寿元.泛议衰老.生命科学,2002,14(2):65~69
3刘怀攀,陈龙,张承烈,等.渗透胁迫和外源ABA对芦苇愈伤组织中3种保护酶活性的影响.植物生理学通讯,2002,38(1):27~29
4杨淑慎,高俊凤.活性氧、自由基与植物的衰老.西北植物学报,2001,21(2):215~220
5蒋选利,李振歧,康振生.过氧化物酶与植物抗病性研究进展.西北农林科技大学学报(自然科学版),2001,29(6):124~129
6田国忠,李怀方,裘维蕃.植物过氧化物酶研究进展.武汉植物学研究,2001,19(4):332~344
7福崎英一郎,小林昭雄.植物における活性酸素障害とその防禦機構.植物の化學調節,1998,33(2):137~147
8中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南. 北京:科学出版社,1999.308~309,314~315,315~316
9黄静.安舒克栓酶的克隆表达及主要性质研究Ⅱ:[硕士学位论文].上海:华东师范大学,2000.5~8
10 曹仪植,宋占午.植物生理学.兰州:兰州大学出版社,1998.372~374
11Levitt J.Responses of plants to environmental stress.New York: Academic Press,1980,35~53
12Dhindsa RS,Matowe W. Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation,J. Exp. Bot,1981,32:79~91
13李国婧,周燮.水杨酸与植物抗非生物胁迫.植物学通报,2001,18(3):295~302
