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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Initial Culture of Cinnamomum caphora (L.) Presl in vitro

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Online published: 2005-02-04

Abstract

In order to get the optimal medium to culture Cinnamomum caphora (L.) Prest. in it, semi-ligneous stem sections of a dominant tree in a mature forest were used as explants and orthogonal layout L9(34) with the compound of three elements (macroelements, BA and NAA) with three levels was adopted in this study. Four weeks later, either axillary sprouts or callus or both were produced by the stem sections cultured in all media. The results showed that the tested three factors have significantly affected the growth of sprouts and callus. Macroelements in MS medium and the highest concentration of BA were the optimal for the growth of sprouts, whereas the optimal conditions to develop callus were the highest concentration of NAA and the lowest concentration of BA. In addition, the sprouts flourished when the ratio of BA/NAA was equal to 40. However, the optimal value of BA/NAA to develop callus was 5 which was the lowest one among the ratios. Although the optimal medium to develop axillary sprouts was MS+BA2.0(mg./L)+NAA0.05(mg./L) and the optimal medium to produce callus was 1/2MS+BA0.5(mg./L)+NAA0.1(mg./L) in this study, the concentration of BA could be increased to develop more sprouts and we could get more callus by increasing concentration of NAA either lower concentration of BA for further study.

Cite this article

Gu Xirong, Huang Jianguo, Yang Qing . Initial Culture of Cinnamomum caphora (L.) Presl in vitro[J]. Chinese Agricultural Science Bulletin, 2005 , 21(2) : 97 -97 . DOI: 10.11924/j.issn.1000-6850.050297

References

1宋爱云,陈辉,董林水.RAPD分子标记在鉴定香樟优选株和普通株中的应用.应用与环境生物学报,2003,9(3):263~265
2索长江,蔡斌华.香樟丛生芽的诱导和快速繁殖研究.林业科技开发,1997, (3):29~30
3连芳青,熊伟,张露.樟树茎段的离体培养和植株再生.江西林业科技,1992, (3):20~21
4石大兴,王米力,石轶松,曾平安,刘碧英.巨桉芽器官离体培养与快繁体系建立的研究.林业科学,2003, 39(1): 69~74
5胡蕙露,杨景华,杨荻荣,王勇.银杏茎段试管培养条件筛选研究.林业科学,2002,38(3):52~56
6谭文澄,戴策刚.观赏植物组织培养技术.北京:中国林业出版社,1991,114
7林萍,普晓兰.滇润楠的组织培养及快速繁殖研究.园艺学报,2003,30(2):239~241
表1培养基配比试验方案
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