Mice oocytes were enucleated during 14~16, 16~18, 18~20 and 20~22 h apart after injected hCG (Human Chorionic Gondaotropin, hCG), so as to research effect on mice somatic cell nucear transfer about enucleation time. Results show that encleation rate of mice oocytes was 80.09 %(338/422), 73.12 %(283 / 387), 55.02 %(230/418), and 48.74 %(193/396) respectively; Activation rate of embryos reconstructed was 66.20 %(190/287), 75.43 %(175 /232), 84.78 %(156/184), 85.14 %(126/148) apart; Blastocyst rate of embryos reconstructed was 13.94 %(40/287), 7.33 %(17/232), 3.80 %(7/184) and 1.35 %(2/148) respectively. It is indicated that enucleation time of mice oocytes is good during 14~16 h after injected hCG.
Cao Hongguo, Zhang Mingfeng, Zhang Yong
. Effect of Enucleation Time on Nuclear Transfer with Cumulus Cells in Mouse[J]. Chinese Agricultural Science Bulletin, 2005
, 21(3)
: 23
-23
.
DOI: 10.11924/j.issn.1000-6850.050323
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