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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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The Pig Transfer Factor Effects on Proliferation of Canine Lymphocytes

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Online published: 2005-03-04

Abstract

Pick-off blood with anticoagulant aseptically and the lymphocytes were isolated. Effects ofFCS,PBMC,PHA-P of different concentration on promote lymphocytes proliferation were tested by orthogonal test. On the base of the best level combination, different concentration pig TF was investigated to detect its effect on the activation of lymphocytes by MTT essay. The result of experiment demonstrate that the best levels combination of canine lymphocyte,PHA-P, calf blood serum were 2.5×106 unit/ml,6.25 μg/ml,10% respectively. Under this optimum cultural condition, the lymphocytes remarkable proliferated between 0.195~25mg/ml of the pig TF,and the best stimulate concentration of the pig TF only or it with PHA-P is 1.56mg/mL. Furthermore, it is confirmed that lymphocytes developed by the pig TF single was much better than it cooperated with PHA-P. The results confirm that the difficult race TF’s effect was obvious and provides a basis for further application on the difficult race TF preventing and curing canine’s virus disease.

Cite this article

Kong Qingbo,, Li Jia, Cong Liyuan, Chen Dekun, Zhang Yanming . The Pig Transfer Factor Effects on Proliferation of Canine Lymphocytes[J]. Chinese Agricultural Science Bulletin, 2005 , 21(3) : 48 -48 . DOI: 10.11924/j.issn.1000-6850.050348

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