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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Ploidy-estimating and Transplanting of regenerated-plants derived from microspore culture of maize

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Online published: 2005-03-04

Abstract

The vigor and rooting of plantlets derived from microspore culture of maize could be obviously improved by adding 1.0mg/L IBA, 0.05mg/L NAA, 1mg/l MET to rooting medium. The transplanting survival rate of plantlets could be increased to 90% through the procedure of hardening plantlets for 24~48h, growing of 5~7d with medium of perlit or vermiculite and then transplanting them to soil supplemented with 1/3 chicken excrement thoroughly decomposed. The survived plants with deep grown color-leaves and vigor roots could grow strongly in the field. The method for measurement of the length of stoma guard cell was proved to be a simple way of estimating ploidy of the regenerated plants.

Cite this article

Lu Ruiju, Wang Yifei, Sun Yuefang, Zhou Runmei, Huang Jianhua . Ploidy-estimating and Transplanting of regenerated-plants derived from microspore culture of maize[J]. Chinese Agricultural Science Bulletin, 2005 , 21(3) : 84 -84 . DOI: 10.11924/j.issn.1000-6850.050384

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