By application of L9(34) orthgonal test and Soxhlet extraction, the total flavon was extracted from fruits,buanchs, leaves of the K. paniculate Laxm. Compared with the two extraction methods, the MAE is suited to the total flavon extracting, and the opimization extracting technique conditions are microwave treatment 1 min.、water bathing 10 min under 60℃ and proportion of solid to liquid in 1:20. The content of total flavon , which extracted from fruits, branches and leaves of the K.paniculate Laxm and determinted with the NaNO2-Al(NO3)3-NaOH coloration system by spectrophotometry at 510nm, were 1.78%、0.27% and 0.45% respectively.The content of total flavon, which extracted from three gathering period time (months of 9, 10, 11of 2003) had been determined and the results were 0.71%、1.78% and 2.70% respectively, shown significant incrreasing tendency within fruit ripening .
Tang Chunyi, Din Wen, Gao Zhongsong
. The Total Flavon Extraction From Fruits, Branchs, Leaves of the Koelreuteria paniculate Laxm
and it,s Content Determination[J]. Chinese Agricultural Science Bulletin, 2005
, 21(5)
: 159
-159
.
DOI: 10.11924/j.issn.1000-6850.0505159
1张睿,徐雅琴,时阳.黄酮类化合物提取工艺研究[J].东北农业大学学报,2002,(3):123~124
2马广恩,申雅雅,鲁学照.栾树属植物化学和生物活性研究概况[J].国外医药-植物药分册,1999,(6):138~142
3窦全琴,袁惠红,王宝松,等.栾树研究的现状及展望[J].江苏林业科技,1999,26,(2):52~53
4梁淑芳,马柏林.栾树化学成份的研究进展[J].西北林学院院报,2004,19,(1):119~122
5许勇,郭兴杰,范捷,等.正交设计研究补骨脂中总黄酮提取工艺[J].沈阳药科大学学报,2003,20(1):144~146
6周谨,闫小燕,贺高红,等.微波提取银杏黄酮苷的方法研究[J].天然产物研究与开发,2003,14(7):42~47
7曾凡骏,陈松波,曾里.一种改进的银杏黄酮紫外分光光度检测方法的研究[J].食品科学,2003,(3):105~107
8田新玲,晏佩霞,仝殿荣.分光光度法测定天然植物中黄酮含量[J].仪器仪表与分析监测,2003,(3):25~26
9罗伟强,廖海达,李小梅,等.四种提取柑皮中黄酮物质的方法比较[J].西部粮油科技,2003,(1):50~51
10 包贝华,杨建平,张丽,等.分光光度法测定介穗炭中总黄酮的含量[J].南京中医药大学学报,2004,20(2):124~125
11 吴谋成主编.食品分析与感官评定[M].北京:中国农业出版社,2002.18~22
12 刘佳佳,李子琪,华辉,等.硼酸－柠檬酸比色法测银杏枝叶中黄酮苷含量[J].食品与发酵工业,1998,25(6):42~43
