Three kinds of newly returned lands from three pre-crops were selected to establish mix-sowing artificial grassland in Three Gorges Region. The growth speed, grass yield, grass proportion and grass weight ratio of 6 grass combinations were determined to analyze the difference between different lands. The results showed that the grass production and grass proportion were significantly influenced by the soils (p<0.01). The grass yield in soybean land was significantly higher than that in rice land and turf land (p<0.01). While the legume grass yields was significantly higher in turf land than that in rice land and soybean land (p<0.01). And the grass production and proportions in different cutting times were changed differently in different lands. The average grass yield in soybean land in second cutting time was increased about 430%. Compared with the first cutting time, the proportion between Festuca arundinacea and Dactylis glomerata in two combinations and two proportions were decreased in second cutting time, except the combination F with 3:7 proportion.