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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Determination of Parathion-methyl in cucumber by ELISA

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Online published: 2005-06-04

Abstract

Using the self-made poly-clonal antibody, the affect of the ion-strength and the methanol concentration to the competitive reaction were investigated, and the reaction system was optimized. An indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of parathion-methyl in cucumber was developed. The results showed that the concentration of parathion-methyl that inhibits 50% of antibody-antigen binding(I50)was 0.13 mg/L, the detection limit I20 was 6.7 μg/L. Most analogues showed negligible cross-reactivity except for fenthion and fenitrothion. Cucumber samples were detected with ELISA and GC after simple pre-procession. The fortified recovery of ELISA were 83.10%~93.46%,CV were 6.61%~10.36%,the determination limit was 0.003mg/kg. The results of the ELISA were verified by GC method.

Cite this article

Han Lijun, Qian Chuanfan, Li Wenming, Jiang Shuren, Wang Xiaoru . Determination of Parathion-methyl in cucumber by ELISA[J]. Chinese Agricultural Science Bulletin, 2005 , 21(6) : 146 -146 . DOI: 10.11924/j.issn.1000-6850.0506146

References

1 Weiler.农药及其代谢物的免疫测定[J].金小雷译.农药译丛,1988,10(3):46~48
2Hass J H, Guardia E J. Production of antibodies against insecticide-protein conjugates[J]. Proc Soc Exp Biol Med, 1968,129:546~551
3Ercegovich C D , Vallejo R P , Gettig R P, et al. Development of a radioimmunoassay for parathion[J]. J. Agric Food Chem.,1981,29:559~568
4刘长武,王一茹,李治祥.对硫磷的人工抗原合成与鉴定[J].环境科学学报,1992,12(3):377~381
5Hunter K W, Lenz D E. Detection and quantification of the organo-phosphate insecticide paraoxon by competitive inhibition enzyme immunoassay[J]. Life Sci., 1982,30:355~356
6Buenafe A C, Rittenburg M B. Combining site specificity of monoclonal antibodies to the organophosphate hapten soman[J]. Mol. Immunol., 1987,24:402~407
7McAdam D P, Hill A S, Beasley H L, et al. Mono-and polyclonal antibodies to organophosphate fenitrothion[J].J. Agric. Food Chem.,1992,40:1466~1470
8 王刚垛,鱼涛,马兆扬,等.甲基对硫磷单克隆抗体的研制及鉴定[J].中华劳动卫生职业病杂志,2001,19(4):265~267
9 王刚垛,何凤生,鱼涛,等.甲基对硫磷ELISA分析方法的建立及初步应用[J].中国工业医学杂志,2001,14(6):327~330
10 钱传范,肖协忠,单国民,等.免疫检测技术在农药残留分析中的应用[J].农药科学与管理,1991,(4):27~32
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