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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Determined Methods of Chlorophyll from Maize

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Online published: 2005-06-04

Abstract

The absorption spectrums and extraction efficiencies of chlorophyll from maize seedling after frozen treatment were compared on six determined methods using different organic solvents. The results showed that the absorption spectrums of five solutions were identical with that of acetone solution, so Arnon’s formula of chlorophyll was suitable for other methods. In house temperature, soaking methods were better than Arnon method. The extraction efficiencies of chlorophyll using mixture solution better than using acetone or ethanol alone, methods of acetone mixed ethanol were preferable for fast extraction and stability. Soaking methods in 50℃ after frozen treatment could accelerate extraction of chlorophyll, but also speed up degradation of chlorophyll, so light screening was necessary.

Cite this article

Li Dexiao, Guo Yuexia, Yun Haiyan, Zhang Min, Gong Xiaoyan, Mu Fang . Determined Methods of Chlorophyll from Maize[J]. Chinese Agricultural Science Bulletin, 2005 , 21(6) : 153 -153 . DOI: 10.11924/j.issn.1000-6850.0506153

References

1苏正淑,张宪政.几种测定植物叶绿素含量的方法比较[J].植物生理学通讯,1989,5:77~78
2陈福明,陈顺伟.混合液法测定叶绿素含量的研究[J].林业科技通讯,1984,(2):4~8
3张宪政.植物叶绿素含量测定-丙酮乙醇混合液法[J].辽宁农业科学,1986,(3):26~28
4洪法水,魏正贵,赵贵文.菠菜叶绿素的浸提和协同萃取反应[J].应用化学,2001,18(7): 532~535
5彭运生,刘恩.关于叶绿素方法的比较研究[J].北京农业大学学报,1992,18(3):247~249
6刘绚霞,董振生,刘创社,等.油菜叶绿素提取方法研究[J].中国农学通报,2004,20(4):62~63
7吴志旭,张雅燕.叶绿素a测定方法的改进及最优提取时间探讨[J].甘肃环境研究与检测,2003,16(2):150~152
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