Objective: observe how differently processed honeysuckle preparations decocted with water and deposited with alcohol effect the movement performance of In- Vitro small Intestine of domestic rabbits; and probe into the scheme of pharmacological action. Method: adopt leaf shell organ experiment method, record the tension data before and after the effect of differently processed honeysuckle preparations on leaf shell small intestine by the BL-New century biologic signal collecting and processing system. Result: At alcohol concentration of 50%, 70%, 90% and medicament concentration of 0.1g/ml, 0.5g/ml, 1.0g/ml respectively, the honeysuckle preparation appears obvious restrain effect on leaf shell small intestine movement of domestic rabbit with a large difference（P<0.11）; At alcohol concentration of 70% and medicament concentration of 1.0%, the honeysuckle preparation appears the most intensive effect(P<0.01); At the same alcohol concentration its restrain effect on small intestine movement increases with the increase of the honeysuckle concentration. Conclusion: Honeysuckle preparation decocted with water and deposited with alcohol has obvious restrain effect on small intestine movement of domestic rabbit; at the same alcohol concentration, its effect becomes more obvious with the increase of honeysuckle preparation concentration.