The polymorphisms of rDNA, mitochondrial DNA (mtDNA) and total genomic DNA were examined in 12 cultivated strains of V. volvacea. By PCR-RFLP technique, two regions of rDNA5.8s+ITS and small mtDNA were successfully amplified, respectively. The amplified products were subsequently digested with special restriction endonucleases. The results showed that genetic diversity was small within the two regions for 12 strains. According to the restriction fragment patterns of rDNA 5.8s+ITS by HinfⅠand Sau3A, 12 strains could be divided into two groups. For small mtDNA, however, all restriction fragment patterns confirmed that there was no variation in 12 strains. The total genomic DNA was then examined by sensitive RAPD technique. The results showed that genetic similarity coefficients between the strains were within a range from 0.554 to 0.898, while average similarity coefficient between them was 0.707. Furthermore, 12 strains could be divided into 4 groups based on the level of similarity coefficient 0.710 by cluster dendrogram. These results provided the genetic foundation for the breeding of V. volvacea.
Yu Zhisheng, Lv Zuozhou, Chen Mingjie, Pan Yingjie
. Studies on DNA Polymorphism of the Cultivated Strains of Volvariella volvacea
by PCR-RFLP and RAPD Techniques[J]. Chinese Agricultural Science Bulletin, 2005
, 21(6)
: 58
-58
.
DOI: 10.11924/j.issn.1000-6850.050658
1刘祖同,罗信昌.食用蕈菌生物技术及运用[M].北京:清华大学出版社,2002.20~64
2陈春涛,张引芳,姚占芳,等.香菇线粒体DNA和核糖体DNA多态性研究初探[J].微生物学杂志,1997,17(2):23~29
3罗信昌,李英波,施庆利.分子生物学技术在菇类研究中的运用[J].食用菌学报,1995,2(4):1~10
4Nicholson M S, Bunyard B A, Royse D J. Phylogeny of the genus Lentinula based on ribosomal DNA restriction fragment length polymorphism analysis[J]. Mycologia,1997,89:400~407
5张引芳，Molina F I. Studies on the differentiation of Lentinula edodes strains by RAPD assay[J]. 食用菌学报,1994,1(1):22~27
6董昌金,江娟,姚占芳.RAPD和酯酶同工酶技术在香菇杂交育种中的应用[J].食用菌学报,2000,7(1):1~7
