RAPD analysisfor mutatedseedsof Tanghui-28 generations, which were returned by No.17 returning satellite,plus its field inspection and isozyme, has been carried out. The results were as follows: (1) abiggest mutatedindividualwhichwas characterizedby selectedgenerations’ richvarieties, stable genetictraits,highyieldingas wellas diseaseresistance.(2) Excellentselectedlines, whichcombinations’yield was much bigger than that of contrast, could be used to make high yielding and disease resistance cross a parental lines. (3) There were 2~4 generations earlier ordinary ,that was also to say ,high space mutation could make the breeding programme develop in advance. (4) Their were signifficant genetic differerces among the mutated generations and its selected generations in some areas, such as esterase isozyme,cytochrome exidase isozyme as well as isozyme vigor. (5) RAPD analysis showed that selected lines by space mutation method varied signifficantly in DNA level.
GONG Zhen-ping, LIU Zi-hua, LIU Gen-qi, MENG Qing-xiang, LIU Zhen-xing
. Studies on Mutagenic Effect of High Space on Sorghum[J]. Chinese Agricultural Science Bulletin, 2003
, 19(6)
: 16
-16
.
DOI: 10.11924/j.issn.1000-6850.030616
[1]蒋兴村,陈芳远. 空间条件对水稻的诱变及其在育种上的应用[M]. 中国的遗传学研究,北京;中国科技出版社,1991.
[2]邓立年. 蔬菜航天诱变育种的初步探讨[J]. 北方园艺,1995,1:9~11.
[3]李源祥,李金国等. 水稻空间技术育种的研究[J]. 遗传,2002,24(4):434~438.
[4]李金国,王培生,张健等. 中国农作物航天诱变育种的进展及前景[J]. 航天医学与医学工程,1999,12(6):465~468.
[5] 李源祥,蒋兴村. 空间条件对水稻恢复系诱变作用的研究[J]. 杂交水稻,1995,5:6~9.
[6] 徐建龙,王俊敏,骆荣廷等. 空间诱变水稻大粒型突变体的遗传育种研究[J]. 遗传,2002,24(4):431~433.
[7] 李金国,潘光堂等. 卫星搭载玉米雄性不育突变体的遗传稳定性研究[J]. 航天医学与医学工程,2002,75(1):51~54.
[8] 顾瑞奇,沈惠明. 空间飞行对小麦种子的生长和细胞学特性的影响[J]. 植物生理学报,1989,15(4):403~407.
[9] 蒋兴村等.“8855”返地卫星搭载对水稻种子遗传特性的影响[J]. 科学通报,1991,36(23):1820~1824.
[10] 刘永宪. 微重力细胞生理学的研究现状及其应用前景[J]. 中国航天,1992,8:3~6.
[11] 陈芳远等. 高空环境对水稻遗传性的影响[J]. 中国水稻科学,1994,8(1):3~6.
[12] 李金国,刘敏等. 蕃茄种子宇宙飞行后的过氧化物同工酶及其RAPD分析[J]. 园艺学报,1999,26(1):33~36.
[13] 李社荣,刘雅男,刘敏等. 空间条件对玉米叶片超微细结构的影响[J]. 核农学报,1998,12(5):274~280.
[14] 李金国,蒋兴村,王长城. 空间条件对几种粮食作物的同工酶和细胞学特性的影响[J]. 遗传学报,1996,23(1):48~55.
[15] Hagen V. Radiationbiologyinspace[J].AdvSpaceRes,1989, 9(10):3~8.
[16] Dutoher F R , Hess E L , Halstead R W. Progressinplantresearchinspace[J].AdvSpaceRes ,1994, 14(8): 159~171.
[17] Porterfied D M,MatthewS W,et al.Spaceflightexposureeffectsontranscription,activity,andlocalization ofalcoholdehydrogenaseinthe rootofArabidopsisthaliana[J].PlantPhysiol,1997, 113(3): 685~693.
[18] Vantwest J S, Paulsen A, et al. in vitro chick pre-Cardiacexplant tissue differentiation during space flight on spacelab-oz[J].Acta Anatomica, 1995, 154(3): 169~180.
[19] 刘自华,刘根齐,龚振平. 高粱空间诱变研究初报[J]. 北京农学院学报,1999,14(1):15~18.
[20] 刘自华,周国顺,龚振平. 高粱空间诱变后代的灰色理论评价[J]. 北京农学院学报,2002,17(3):6~9.
[21] 刘根齐,龚振平,刘春光等. 高粱空间诱变选育的同工酶及RAPD分析[J]. Developmental andreproductive biology. 2002,11(4):270~275.