Rose (Rosa rugosa Thunb.) is an important ornamental, but the fast propagation system hasn’t been set up yet. In this paper, we studied the effect of different light intensity on browning of rose initially cultured in vitro. Our results showed that the browning index of rose decreased and the percentage of germination increased after field treatment, especially in the light intensity of 5,333±1,751 Lx. Compared with the light intensity of 5,333±1,751 Lx and 63,078±1,651 Lx, the activity of PPO of rose under natural light intensity showed no significant difference whereas the content of phenolic substances was much higher than those treated with shaded illumination. The co-efficiency analysis demonstrated that there was no obvious relation between the activity of phenol oxidase and content of phenolic substances.
Yang Bo, Han Zhenhai , Zhang Yong, Li Tianhong
. Effect of Different Light Intensity on Browning of Rose (Rosa rugosa) Initially Cultured in vitro[J]. Chinese Agricultural Science Bulletin, 2003
, 19(6)
: 194
-194
.
DOI: 10.11924/j.issn.1000-6850.0306194
1 陈秀芳, 王坤范. 桃果实发育中褐变因子变化规律的研究. 园艺学报, 1995, 22: 230-234
2 Bonga J M, Durzan D J. 树木组织培养. 阙国宁, 郭达初, 李金田译. 北京: 中国林业出版社, 1988. 25-26, 141-143, 175
3 Das S, Jha T B, Jha S. In vitro micropropagation of cashewnut. Plant Cell Reports, 1996, 15: 615-619
4 高国训. 植物组织培养中的褐变问题. 植物生理学通讯, 1999, 35: 501-506
5 Marks T R, Simpson S E. Reduced phenolic oxidation at culture initiation in vitro following the exposure of field-grown stock-plants to darkness or low level of irradiance. J. Hort. Sci., 1990, 65: 103-111
6 Creasy L L. The increase in phenylalanine ammonia-lyase activity in strawberry leaf disks and it’s correlation with flavonoid synthesis. Phytochemistry, 1968, 7: 441-446
7朱广廉，钟海文，张爱琴主编. 植物生理学实验. 北京农业大学出版社，1990, 37-39
8 Kubota N. Phenolic content and L-phenylalanine ammonia-lyse activity. 1995, In Linskens H.F. and J.F. (Eds). Fruit Analysis, Spinger-Verlag, Heidelberg, 1995, 82-83
9 兰海燕. 向日葵离体培养植株再生的影响因素研究. 新疆农业科学, 2000, 31: 163-165
10李焕秀，王乔春，李春秀等. 梨芽和茎尖多酚氧化酶活性和总酚含量的初步研究. 四川农业大学学报, 1994, 2: 218-222
