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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Analysis of the mutants of Fengjie navel orange(Citrus sinensis Osbeck)by AFLP

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Online published: 2003-12-24

Abstract

Genetic variabilities of the low-acid mutants and late-mature mutant of the Fengjie navel orange were examined by AFLP technology .By using 64 pairs of selective primers ,5336 bands ,among them , 7 specific bands in low-acid mutant ,2 specific bands in late-mature mutantwere detected. The sequencing of these specific bands and their Blastp analysis showed :the trait of low acid in fruit in the low-acid mutant of Fengjie navel orange could result from the deficiency or mutation of the gene encoding Na+/H+ antiporter-like protein, while the deficiency or mutation of the genes encoding the flower development protein cycloidea (cyc3) and cytoplasmic aconitate hydratase could be responsible for the late mature of late-mature mutant .

Cite this article

,Luo An-cai,Li Chun-fan ,Huang ren-hu,Li Dao-gao . Analysis of the mutants of Fengjie navel orange(Citrus sinensis Osbeck)by AFLP[J]. Chinese Agricultural Science Bulletin, 2003 , 19(6) : 20 -20 . DOI: 10.11924/j.issn.1000-6850.030620

References

[1] 罗安才,李道高,杨晓红. 柑橘果实有机酸代谢研究进展. 园艺学报,2001, 28 (增刊):597--602(in Chinese)
Luo A C, Li D G, Yang X H. Advances of the study on the metabolism of organic acids in Citrus fruits.Acta HorticulturaeSinica.2001,28 (Supplement):597~602
[2 ] 沈德绪,王元裕,陈力耕. 柑桔遗传育种学. 北京:科学出版社. 1998, P204-205
Shen D X,Wang Y Y,Chen L G. The genetics and breeding of citrus. Beijing;Scientifical press.1997,P204-205
[3] 王关林,方宏筠. 植物基因工程原理与技术 北京:科学出版社,1998,P598-602
Wang G L,Fang H J. The principle and technics of plant gene engineering. Beijing; Scientifical press.1998,P598-602
[4] Sadka A, Dahan E, Cohen L, Marsh K B.Aconitase activity and expression during the development of lemon fruit. Physiology Plant, 2000,108: 255-262.
[5] Müller M.L., Irkenskiesecker U., L Taiz . On the mechanism of hyperacidification in lemon –Comparison of the vacuolar H+-ATPase activities of fruits and epicotyls. J. Biological Chemics, 1996, 271: 1916~1924
[6] BruneA,Gonzalez P C, Echeverria E.Citrate uptake into tonoplast vesicles from acid lime (Citrus aurantifolia) juice cells. Journal of Membrane Biology, 1998,166: 197~203
[7].Marsh K B, Emer Y, Gonzalez P G, Echeverria E. Role of the proton-pumping vacuolar pyrophosphalase in acid metabolism of citrus. ISC Congress,2000,3-7 December:160
[8] Fang D Q ,Federici C T,Roose M L. Development of molecular makers linked to a gene controlling fruit acidicity in citrus. Genome, 1997,40:841-849
[9]Canel, C, Bailey-Serres J N, Roose M L. In Vitro [14C] Citrate Uptake by Tonoplast Vesicles of Acidless Citrus Juice Cells. Journal of America Horticultural Science Society, 1995,120 (3) : 510-514
[10] 罗安才,杨晓红,李道高等 柑桔果实发育过程中有机酸含量及相关代谢酶活性的变化 中国农业科学(已收用)
Luo A C, Yang X H, Deng Y Y, Li C F, Xiang K S, Li D G. Organic Acid Concentrations and the Relative Enzymatic Changes During the Development of the Citrus Fruits. Scientia Agricultura Sinica(submitted)
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