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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Study on Transgenic Bt Cotton Using Kanamycin as an Indirect Identification Technique Indoors

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Online published: 2003-12-24

Abstract

A simple,cheap,and efficient technique for transgenic Bt cotton using kanamycin as an indirect identification marker in the laborotary is tested.The result showed that kanamycin could be used in the laborotary to test whether or not a plant is resistant.Seed of transgenic Bt cotton were cultured on sand bed containing 4000mg/L kanamycin solution for six days.The cotyledons of resistant varieties remained normal green color,whilst these of the commercial varieties all turned yellow.This technique is especially usful for the purity test of transgenic Bt cotton before the seed is sold. Meanwhile,the hypocotyls and fresh weigh of the cotton seedling were affected by the kanamycin in some degree.

Cite this article

Zhu Jiabao,Yang Kesheng,Chan Yankun,Liu Fangzhi,Jiang Benli,Xu Daoqing . Study on Transgenic Bt Cotton Using Kanamycin as an Indirect Identification Technique Indoors[J]. Chinese Agricultural Science Bulletin, 2003 , 19(6) : 69 -69 . DOI: 10.11924/j.issn.1000-6850.030669

References

1 李燕娥,焦改丽.转基因棉花纯度快速测定法[J].中国棉花,1998,25(8):14-18.
2 王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998.
3 孙敬,唐灿明,袁小玲等.利用卡那霉素间接鉴定法进行大规模的棉花转基因育种技术[J].棉花学报,2000,12(5):270-276.
4 马丽华,许红霞,胡育昌.转基因棉卡那霉素田间快速鉴定法[J].中国棉花,2000,27(12):11-12.
5 杨可胜,产焰坤,陈军等.棉花抗虫性鉴定技术研究[J].安徽农业科学,2002,30(2):193-194.
6 王坤波,张香娣,刘方等.子叶期卡那霉素快速鉴定转基因棉花[J].中国棉花,2001,28(2):20-21
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