Adventitious buds were inducted almost without the development of calli with the hypocotyl segments and cotyledons of aseptic seedlings of Brassica oleracea L. var. capitata as explants. Cultured on the MS medium supplemented with 6-BA, its propagation rate was highest. The optimum medium for rooting was 1/2MS supplemented with 0.5mg/L IBA and 0.1mg/L NAA. High proportion of sucrose and agar in the medium and intense lightening could control the occurrence of glassy shoot effectively.