Using the stems, leafstalks, leaves of Dioscorea Zingbernisis as the explants in tissue culture, the result shows that stems and leafstalks are the best. The best culture medium of callus induction is MS+2,4-D 2.0mg/L+BA 0.5mg/L; The differentiation on culture medium is MS+BA 3.0mg/L+NAA 0.2mg/L；The subculture medium is MS+BA 1.0mg/L+NAA 0.2mg/L;Root medium is MS+NAA 1.0mg/L.Tube seedings of Diosocrea Zingibernisis were treated with colchinine. The result shows that the best induction rate could reach 50% after treatment with 0.15% colchcine in 24 hours. Compared with the polyploid plants were thicker and larger. A few leaves appeared malformed .The stoma become larger and less in number. Cytological study show that the chromosome numbers of variation is 40,that of normal diploids is 20.