The extenders and the cryoprotectant contents of frozen semen pellets were studied using the plate in boar. The results showed that the number 7 was the best extender (p<0.05)in seven groups, and the best cryoprotectant was glycerol (p<0.01).When the glycerol content was 2 percent (p<0.05),the sperm motility of frozen semen pellets was best in all treatment groups.The method of dry thawn of frozen semen pellets was better than that of wet thawn(p<0.05); the sperm motility was 0.46-0.49 after the frozen semen pellet was thawed at 40to 45 ℃. supplyment in the extender could increase the thawn sperm survival time and motility effectively(p<0.01).
JIANG Zhong-liang ,LI Qing-wang ,HU Jian-hong , WANG Li-qiang , YU Yong-sheng , XIA Fan
. Study on the Technique of Boar Semen Freezing[J]. Chinese Agricultural Science Bulletin, 2004
, 20(4)
: 12
-12
.
DOI: 10.11924/j.issn.1000-6850.040412
[1] Polge C. Artificial insemination in pigs [J]. Vet Pec,1956, 68:62-76。
[2] Pursel V G, Johnson L A. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure [J]. Joural of Animal Science,1975,40:99—102。
[3] 李震中,杨学时,冯健超,等.家畜繁殖技术进展 [M].重庆:技术文献出版社重庆分社,1976。
[4] 曹礼,邢喜生,牛子成,等.猪浓缩冻精的研究[J].养猪,1991,(3):22-23。
[5] 黄全成,刘丑生,康文彪,等.猪冷冻精液新技术研究试验报告 [J].甘肃畜牧兽医,1993,23(6):4-6。
[6] 龙翔,邰秀林.冷冻保护剂在猪精液冷冻中的作用分析 [J].畜禽业,1999,(9):19-22。
[7] 周佳勃,岳奎忠,孙兴参,等.猪精液冷冻技术的研究 [J].中国兽医学报,2002,22 (5):295-299。
[8] Johnson L A, Weitze K F , Fiser P. Storage of boar semen. Animal Reproduction Science,[J]. 2000,62:143-172。
[9] Paulenz H,Taugbol O. Effect of dietary supplementation with cod liver oil on cold shock and freezability of boar semen. [J]. 1999,34 (5):431-435。
[10] Pursel . Animal Reproduction-BARC Symposia Vernon G.,[J].1979,148-157.
[11] Lake P E,Stewart J M. Further studies on rapid diluting and warming of boar semen[J].British Poultry Science, 1978,19:187-194。
[12] Fahy G M. Effect of rapid warming of boar semen on sperm morphology and physiology [J]. Cryobiology,1986, 23:1-13。
[13] 徐章龙,蒋伟华,蔡加乐等. 猪精液冷冻技术研究 [J].中国畜牧杂志,1999,(4):28-32。
[14] 郭年潘.猪精液常温保存 [J].国外畜牧科技,1990,(6):21-22。