Isoelectric focusing was used to identify the seed purity of hybird rice.The study shown that at least one pair of protein bands with parants characteristics were found in hybird seeds of Boyou 998.The testing results, which was made in lab by distinguishing protein bands with parants characteristics, are much similar to those from field testings.Consequently, isoelectric focusing could be potential technique applied to identify the seed purity of hybird rice in the future.
HU Qun-bao, XIA Qing-hua, CHEN Sen, CAI Hui-jiao，He De-ying
. Identification of Seed Purity from Hybird Rice (Oryza spp.) of Variety Boyou 998[J]. Chinese Agricultural Science Bulletin, 2004
, 20(4)
: 158
-158
.
DOI: 10.11924/j.issn.1000-6850.0404158
陈洪，钱前，朱立煌，闵绍楷，陈美玲，杂交水稻汕优63杂种纯度RAPD鉴定。科学通报，1996，41(9)：833-836.
钱前，陈洪，孙宗修，颜红岚.真假杂交水稻II优63的RAPD鉴定.中国水稻科学，1996, 10(4): 241-242.
曾大力, 钱前, 何平, 颜红岚, 孙宗修, 朱立煌. 用分子生物学技术鉴别真、假杂交稻的研究. 中国农业科学,1999,32(2):93-97.
彭锁堂，庄杰云，颜启传，郑康乐.我国主要杂交水稻组合及其亲本SSR标记和纯度鉴定.中国水稻科学, 2003, 17(1):1～5.
詹庆才.利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究.杂交水稻，2002，17(5):46-50.
Radola B J， Ultrathin layer isoelectric focusing in 50-100um polyacrylamide gels on silanized glass plates or polyester files.Electrophoresis, 1980, 1:43-56.
严敏，王晓峰.杂交玉米、水稻和辣椒种子品种真实性和纯度的室内快速鉴定.华南农业大学学报(自然科学版)，2003, 24(2): 6-8.
王晓峰、黄惠玲、R. Knoblauch、N. Leist.超薄等电聚焦电泳技术在水稻品种鉴定上的运用. 种子，2000，(4):6-9.
王晓峰、赵婷，超薄等电聚焦电泳技术及其在种子纯度鉴定中的运用. 种子，2000，(6):55-57。
蒋昌华、王和勇、陈润政、刘永栋，利用等电聚焦技术鉴定杂交水稻种子纯度，种子世界，2001，Vol. 224（8）：23-24。
Reiner Westermeier, Electrophoresis in Practice, Third Edition, 2001, 171-182, WILEY-VCH.
