Techniques for in vitro production (IVP) of buffalo embryos adopting the procedures developed in cattle have received increasing interest in the recent times. A high oocyte maturation, fertilization and cleavage rate and a low rate of blastocyst yield and calving following transfer of in vitro produced buffalo embryos have been obtained. The efficiency of IVP in buffalo is much lower than that in cattle. Several problems need to be resolved before IVP technology can be used regularly in buffalo breeding. This review attempts to present an overview of the different techniques used in buffalo to produce transferable embryos in vitro, namely in vitro maturation and fertilization of immature oocytes and in vitro development of the resulting cleaved embryos to the blastocyst stage before transfer. The problems associated with IVP and the possible solutions discussed.
Sun Yan, Zhang Jia-hua
. in vitro production of buffalo embryos[J]. Chinese Agricultural Science Bulletin, 2004
, 20(4)
: 17
-17
.
DOI: 10.11924/j.issn.1000-6850.040417
[1]. Madan ML, Das SK, Palta P.Application of reproductive technology to buffaloes. Anim Reprod Sci 1996, 42, 299~306.
[2]. Nandi S, Chauhan MS, Palta P.Influence of cumulus cells and sperm concentration on cleavage rate and subsequent embryonic development of buffalo (Bubalus bubalis)oocytes matured and fertilized in vitro. Theriogenology. 1998, 50,1251~1262.
[3]. Nandi S, Ravindranatha BM, Gupta SP, et al. Timing of sequential changes in cumulus cells and first polar body extrusion during in vitro maturation of buffalo oocytes. Theriogenology. 2002, 57,1151~1159.
[4]. Galli C, Crotti G, Notari C, et al. Embryo production by ovum-pick up from live donors. Theriogenology. 2001, 55, 1341~1357.
[5]. Madan ML, Singla SK, Jailkhani S, et al. In vitro fertilization and birth of first ever IVF buffalo calf. In: Proc Third World Buffalo Congress, Varna, Bulgaria. 1991, l7, 11~17.
[6]. Farin PW, Crosier AE, Farin CE.In.uence of in vitro systems on embryo survival and fetal development in cattle. Theriogenology.2001, 55, 151~170.
[7]. Das GK, Jain GC, Solanki VS, et al. Efficacy of various collection methods for oocyte retrieval in buffalo. Theriogenology. 1996, 46, 1403~1411.
[8]. Totey SM, Singh G, Taneja M, et al. In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis).J Reprod Fertil. 1992, 95, 597~607.
[9]. Gordon I, Lu KH.Production of embryos in vitro and its impact on livestock production. Theriogenology 1990, 33, 77~87.
[10]. Aboul-Ela MBE.Superovulation in the buffaloes: constraints and manipulation. Buffalo J. 2002, 16, 1–20.
[11]. Gupta PSP, Nandi S, Ravindranatha BM.In vitro culture of buffalo (Bubalus bubalis) preantral follicles. Theriogenology. 2000, 57:87~99
[12]. Chauhan MS, Singla SK, Palta P, et al. In vitro maturation and fertilization, and subsequent development of buffalo (Bubalus bubalis) embryos: effects of oocyte quality and type of serum. Reprod Fertil Dev. 1998, 10,173~177.
[13]. Das SK, Chauhan MS, Palta P, et al. Influence of cumulus cells on in vitro maturation of denuded buffalo oocytes. Vet Rec. 1997, 141, 522~523.
[14]. Raghu HM, Nandi S, Reddy SM.Development of buffalo oocyte to the blastocyst stage in vitro in serum free, semi-defined media: effect of insulin-transferrin-selenium and epidermal growth factor. Vet Rec. 2002, 146, 147~151
[15]. Totey SM, Pawshe CH, Singh GP.Effects of bull and heparin and sperm concentration on in vitro fertilization of buffalo (Bubalus bubalis) oocyte matured in vitro. Theriogenology. 1993, 39, 887–898.
[16]. Tervit HR, Whittingham DG, Rowson LEA.Successful culture in vitro of sheep and cattle ova. J Reprod Fertil. 1972, 30, 493~497.
[17]. Jian TS, Sheng YN, Shun SD, et al.Application of bovine in vitro fertilization procedures to buffalo. J Guangxi Agril University.1998, 17, 312~317.
[18]. Gardner DK, Lane M.Culture of viable human blastocysts in defined sequential serum-free media. HumReprod 1998, 13, 101~102.
[19]. Lane M.Mechanism for managing cellular and homeostatic stress in vitro. Theriogenology 2001, 55, 225~236.
