The rhizosphere nutrition in two kinds of soil (pH4.6 or 6.8) was studied in two inbred lines of maize (Z01: Al-tolerant, Z02: Al-sensitive) by the means of 3-compartment root box. The available Al、P、Ca content and distribution in root region showed difference between two kinds of soil with different pH, but the content and distribution of available K was basically same. The Al content in the acid soil near the root (0-1cm) was significantly higher than that in far-from-root region. The Al content in soil of root region of Al-tolerant inbred lines Z01 was significant higher than that of Al-sensitive ones, and Al-tolerant inbred lines Z01 had strong excluding capability to Aluminum. There was a P gradient in 0-4 cm region that declined from far-from-root region to root surface in two inbred lines in neutral soil, but the P declined gradient only exist in 0-2.5 cm region near root surface in acid soil. In acid soil, the P content of Al-tolerant inbred lines Z01 in 1-2.5 cm region near root surface was relative higher and mobilizing ability to P was relative stronger. The Ca content of root region in the acid soil was significant lower than that of neutral soil. In acid soil, the Ca、Mg absorbing capability of the roots in Al-tolerant inbred lines Z01 was relatively stronger, and an obvious Ca declined gradient in 0-2.5 cm region from far-root region to root surface was found in tolerant line, but in sensitive lines Z02 it had a small change.