In order to shorten the detection time of spectrophotometry for determination of organophosphate and carbamate pesticide residues in vegetables, the paper studied the optimum conditions when 10 minutes was chosen as the incubation time. The new criterion for the optimum conditions was put forward , that the optimum conditions was the incubation temperature and the pH when the enzyme inhibition rate was maximum, that the precondition was enzyme was on certain activation level（0.1333 min-1 ≤k1 ≤0.2667min-1 [ 1 ]）.The optimum incubation temperature was 47℃and the optimum pH of phosphate buffer was 7.7 when 10 minutes was chosen as the incubation time. The new criterion of the detection result in the optimum conditions was put forward ,that vegetable contained certain organophosphate or carbamate pesticide residues, if enzyme inhibition rate≥58%.
Yang Dongpeng，Zhang Chunrong，Dong Min， Du Xiangge
. The Research and Improvement of Spectrophotometry for Determination of
Organophosphate and Carbamate Pesticide Residues in Vegetables*[J]. Chinese Agricultural Science Bulletin, 2004
, 20(4)
: 58
-58
.
DOI: 10.11924/j.issn.1000-6850.040458
1高晓辉，等．蔬菜上农药残毒快速检测技术——酶抑制法检测有机磷和氨基甲酸酯类农药．农药科学与管理，2000，21（3）：29～31
2 GB/T5009．199-2002《蔬菜中有机磷和氨基甲酸酯类农药残留量快速检测方法》（卫生部标准）；
3 NY/T448-2001《蔬菜上有机磷和氨基甲酸酯类农药残留量快速检测方法》（农业部标准）；
4 GB8321.1-87《农药合理使用标准（一）》；GB8321.2-87《农药合理使用标准（二）》；GB8321.5-87《农药合理使用标准（五）》
